Cereal Vinegar Sediment Modulates Gut Microbiota and Microbiota-Associated Metabolic Profiles in Mice with Short-Term High-Fat Diet Exposure
5 affiliations
- 1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
- 2Affiliated Hospital of Jiangnan University, Wuxi 214122, China
- 3Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, China
- 4College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China
- 5State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Abstract
Background: Cereal vinegar sediment (CVS), a byproduct formed during traditional grain vinegar storage, possesses lipid-lowering, glucose-lowering, and antioxidant properties. However, its effects on gut microbial and metabolic responses to short-term high-fat diet (HFD) exposure remain unclear. This study aimed to characterize the compositional profiles of Dade CVS (DD-CVS) and Hengshun CVS (HS-CVS) and investigate their effects on host metabolism and gut microbiota in mice with short-term HFD exposure. Methodology: The physicochemical properties of CVSs were evaluated, and their chemical compositions were characterized via untargeted metabolomics. Mice were fed a short-term HFD and administered DD-CVS or HS-CVS. Fecal microbial communities were characterized by 16S rRNA gene sequencing, whereas cecal contents and serum were analyzed using untargeted metabolomics. Results: Both CVSs were rich in amino acids, their derivatives, and polyphenolic compounds. CVS treatments attenuated body-weight gain and reshaped the gut microbiota composition of mice, including changes in the relative abundance of Allobaculum, Akkermansia, and Lachnospiraceae_Clostridium. Differentially, DD-CVS enriched Blautia and Ruminococcus, whereas HS-CVS enriched Bacillus. The results of the gut microbial metabolic profiles showed that DD-CVS treatment regulated amino acid metabolism, while HS-CVS treatment influenced bile acid and arachidonic acid metabolism. Furthermore, serum metabolomics revealed that CVSs modulated systemic metabolic profiles and enriched CVS-associated compounds, such as α-linolenic acid. The candidate microbiota-sensitive metabolites, including lithocholic acid and 9(S)-HPODE, showed significant correlations with specific microbial taxa. Conclusions: DD-CVS and HS-CVS induced distinct gut microbial and metabolic responses during short-term HFD exposure, supporting further evaluation of CVS as a potential functional food.
Related
- Fecal microbiota profiles and targeted qPCR markers associated with the need for therapeutic ascites drainage in patients with terminal pancreatic cancer: an exploratory study
- Postelectroacupuncture Fecal Microbiota Transplantation (post‐EA FMT) Regulates Gastrointestinal Hormones in Functional Dyspepsia Rats Is Associated With Modulation of the Intestinal Microbiota‐Bile Acid Metabolic Pathway
- Structural Features of a Dancong Tea Polysaccharide and Its Amelioration of Glucolipid Metabolic Disorders Associated with Gut Microbiota Modulation.
- Small-Intestinal Motility Interrelates With Fecal Microbiota, Metabolites and Metabolic Tissue Gene Expression in Metabolic Dysfunction-Associated Steatotic Liver Disease
- Gut virome remodeling after fecal microbiota transplantation is associated with virus-bacteria co-variation and metabolic response in obesity and type 2 diabetes.
BibTeX
@article{duan2026cereal,
title = {{Cereal Vinegar Sediment Modulates Gut Microbiota and Microbiota-Associated Metabolic Profiles in Mice with Short-Term High-Fat Diet Exposure}},
author = {Wen-Hui Duan and Yi-Lin Ren and Qi-Jie Guan and Jin-Song Shi and Zheng-Hong Xu and Yu-Zheng Xue and Cheng-Cheng Zhang and Yi Guo and Yan Geng},
journal = {Metabolites},
year = {2026},
month = oct,
doi = {10.3390/metabo16110808},
url = {https://www.mdpi.com/2218-1989/16/11/808},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Cereal Vinegar Sediment Modulates Gut Microbiota and Microbiota-Associated Metabolic Profiles in Mice with Short-Term High-Fat Diet Exposure
AU - Wen-Hui Duan
AU - Yi-Lin Ren
AU - Qi-Jie Guan
AU - Jin-Song Shi
AU - Zheng-Hong Xu
AU - Yu-Zheng Xue
AU - Cheng-Cheng Zhang
AU - Yi Guo
AU - Yan Geng
PY - 2026
DA - 2026/10/22
JO - Metabolites
DO - 10.3390/metabo16110808
UR - https://www.mdpi.com/2218-1989/16/11/808
AB - Background: Cereal vinegar sediment (CVS), a byproduct formed during traditional grain vinegar storage, possesses lipid-lowering, glucose-lowering, and antioxidant properties. However, its effects on gut microbial and metabolic responses to short-term high-fat diet (HFD) exposure remain unclear. This study aimed to characterize the compositional profiles of Dade CVS (DD-CVS) and Hengshun CVS (HS-CVS) and investigate their effects on host metabolism and gut microbiota in mice with short-term HFD exposure. Methodology: The physicochemical properties of CVSs were evaluated, and their chemical compositions were characterized via untargeted metabolomics. Mice were fed a short-term HFD and administered DD-CVS or HS-CVS. Fecal microbial communities were characterized by 16S rRNA gene sequencing, whereas cecal contents and serum were analyzed using untargeted metabolomics. Results: Both CVSs were rich in amino acids, their derivatives, and polyphenolic compounds. CVS treatments attenuated body-weight gain and reshaped the gut microbiota composition of mice, including changes in the relative abundance of Allobaculum, Akkermansia, and Lachnospiraceae_Clostridium. Differentially, DD-CVS enriched Blautia and Ruminococcus, whereas HS-CVS enriched Bacillus. The results of the gut microbial metabolic profiles showed that DD-CVS treatment regulated amino acid metabolism, while HS-CVS treatment influenced bile acid and arachidonic acid metabolism. Furthermore, serum metabolomics revealed that CVSs modulated systemic metabolic profiles and enriched CVS-associated compounds, such as α-linolenic acid. The candidate microbiota-sensitive metabolites, including lithocholic acid and 9(S)-HPODE, showed significant correlations with specific microbial taxa. Conclusions: DD-CVS and HS-CVS induced distinct gut microbial and metabolic responses during short-term HFD exposure, supporting further evaluation of CVS as a potential functional food.
SN - 2218-1989
ER -