Cotinus coggygria Scop. extract-loaded polycaprolactone/hyaluronic acid nanofibers as candidate wound dressings: in vitro characterization, cytocompatibility and antibacterial evaluation
5 affiliations
- 1Department of Pharmaceutical Technology, Faculty of Pharmacy, Gazi University
- 2Department of Pharmaceutical Technology, Faculty of Pharmacy, Başkent University
- 3Department of Bioengineering, Faculty of Engineering, Kırıkkale University
- 4Department of Pharmacognosy, Faculty of Pharmacy, Gazi University
- 5Department of Pharmacognosy, Faculty of Pharmacy, Lokman Hekim University
Abstract
Cotinus coggygria Scop. (Anacardiaceae) possesses antibacterial, antioxidant, anticancer, and wound-healing properties. Hyaluronic acid (HA), a key extracellular matrix component, supports tissue regeneration. In this study, electrospun polycaprolactone (PCL) nanofibers containing C. coggygria extract and HA were developed as wound dressings. Optimized polymer solutions and electrospinning parameters were used according to material composition. Nanofibers were characterized by contact angle measurements, texture analysis, scanning electron microscopy, and in vitro cell culture assays. The fiber diameters ranged from 300 to 700 nm, and contact angle decreased to 65.3°, indicating improved hydrophilicity due to HA and C . coggygria extract incorporation. The addition of extract and HA yielded a maximum tensile strength of 2.40 MPa. After 24 h of exposure, the F5 maintained L929 fibroblast viability above 80% across the entire tested range (0.062–1 mg/mL). At 48 h, F5 produced the lowest apoptotic (6% ± 1%) and necrotic (10% ± 1%) indices among the extract-containing fibers at 1 mg/mL, indicating cytocompatibility towards L929 fibroblasts under the conditions tested. Antibacterial tests revealed inhibition zones of 21 ± 0 mm against Staphylococcus aureus and 6 ± 1 mm against Escherichia coli . Overall, PCL nanofibers incorporating C. coggygria extract and HA demonstrate promising potential for wound healing applications owing to their favorable mechanical properties, high biocompatibility, and notable antibacterial activity.
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BibTeX
@article{saralacarca2026cotinus,
title = {{Cotinus coggygria Scop. extract-loaded polycaprolactone/hyaluronic acid nanofibers as candidate wound dressings: in vitro characterization, cytocompatibility and antibacterial evaluation}},
author = {Eylul Su Saral-Acarca and Serdar Tort and Mustafa Türk and Fatma Sezer Senol Deniz and Ilkay Erdogan Orhan and Sibel Ilbasmis-Tamer},
journal = {Frontiers in Pharmacology},
year = {2026},
month = oct,
doi = {10.3389/fphar.2026.1948812},
url = {https://www.frontiersin.org/articles/10.3389/fphar.2026.1948812/full},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Cotinus coggygria Scop. extract-loaded polycaprolactone/hyaluronic acid nanofibers as candidate wound dressings: in vitro characterization, cytocompatibility and antibacterial evaluation
AU - Eylul Su Saral-Acarca
AU - Serdar Tort
AU - Mustafa Türk
AU - Fatma Sezer Senol Deniz
AU - Ilkay Erdogan Orhan
AU - Sibel Ilbasmis-Tamer
PY - 2026
DA - 2026/10/23
JO - Frontiers in Pharmacology
DO - 10.3389/fphar.2026.1948812
UR - https://www.frontiersin.org/articles/10.3389/fphar.2026.1948812/full
AB - Cotinus coggygria Scop. (Anacardiaceae) possesses antibacterial, antioxidant, anticancer, and wound-healing properties. Hyaluronic acid (HA), a key extracellular matrix component, supports tissue regeneration. In this study, electrospun polycaprolactone (PCL) nanofibers containing C. coggygria extract and HA were developed as wound dressings. Optimized polymer solutions and electrospinning parameters were used according to material composition. Nanofibers were characterized by contact angle measurements, texture analysis, scanning electron microscopy, and in vitro cell culture assays. The fiber diameters ranged from 300 to 700 nm, and contact angle decreased to 65.3°, indicating improved hydrophilicity due to HA and C . coggygria extract incorporation. The addition of extract and HA yielded a maximum tensile strength of 2.40 MPa. After 24 h of exposure, the F5 maintained L929 fibroblast viability above 80% across the entire tested range (0.062–1 mg/mL). At 48 h, F5 produced the lowest apoptotic (6% ± 1%) and necrotic (10% ± 1%) indices among the extract-containing fibers at 1 mg/mL, indicating cytocompatibility towards L929 fibroblasts under the conditions tested. Antibacterial tests revealed inhibition zones of 21 ± 0 mm against Staphylococcus aureus and 6 ± 1 mm against Escherichia coli . Overall, PCL nanofibers incorporating C. coggygria extract and HA demonstrate promising potential for wound healing applications owing to their favorable mechanical properties, high biocompatibility, and notable antibacterial activity.
SN - 1663-9812
ER -