Evaluation of the Adjuvant Activity of Herniaria glabra Saponin-Based Nanoadjuvants
- 1Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan
Abstract
The increasing use of subunit and recombinant vaccines has created a demand for safe and effective adjuvants capable of enhancing immune responses. Here, we investigated a saponin-rich fraction isolated from Herniaria glabra as a source of novel nanoparticulate vaccine adjuvants. The saponins spontaneously self-assembled with lipid components to form stable nanocomplexes with favorable physicochemical characteristics. In a murine immunization model using viral glycoprotein antigens, these nanocomplexes significantly enhanced antigen-specific immune responses, increasing antibody titers by 8- to 16-fold compared with antigen alone. Their adjuvant activity exceeded that of aluminum hydroxide and approached that of Quillaja saponaria-derived saponin formulations. The nanocomplexes also induced balanced cellular immune responses comparable to those reported for Quil A-based nanosystems. These findings identify H. glabra as a promising alternative source of bioactive saponins and demonstrate the potential of plant-derived nanocomplexes as versatile adjuvant platforms for next-generation vaccines.
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BibTeX
@article{bogoyavlenskiy2026evaluation,
title = {{Evaluation of the Adjuvant Activity of Herniaria glabra Saponin-Based Nanoadjuvants}},
author = {Andrey Bogoyavlenskiy and Madina Alexyuk and Pavel Alexyuk and Elmira Omirtayeva and Irina Zaitseva and Yergali Moldakhanov and Elmira Anarkulova and Kuralay Akanova and Timur Kerimov and Aziza Temirbayeva and Vladimir Berezin},
journal = {Molecules},
year = {2026},
month = oct,
doi = {10.3390/molecules31193523},
url = {https://www.mdpi.com/1420-3049/31/19/3523},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Evaluation of the Adjuvant Activity of Herniaria glabra Saponin-Based Nanoadjuvants
AU - Andrey Bogoyavlenskiy
AU - Madina Alexyuk
AU - Pavel Alexyuk
AU - Elmira Omirtayeva
AU - Irina Zaitseva
AU - Yergali Moldakhanov
AU - Elmira Anarkulova
AU - Kuralay Akanova
AU - Timur Kerimov
AU - Aziza Temirbayeva
AU - Vladimir Berezin
PY - 2026
DA - 2026/10/02
JO - Molecules
DO - 10.3390/molecules31193523
UR - https://www.mdpi.com/1420-3049/31/19/3523
AB - The increasing use of subunit and recombinant vaccines has created a demand for safe and effective adjuvants capable of enhancing immune responses. Here, we investigated a saponin-rich fraction isolated from Herniaria glabra as a source of novel nanoparticulate vaccine adjuvants. The saponins spontaneously self-assembled with lipid components to form stable nanocomplexes with favorable physicochemical characteristics. In a murine immunization model using viral glycoprotein antigens, these nanocomplexes significantly enhanced antigen-specific immune responses, increasing antibody titers by 8- to 16-fold compared with antigen alone. Their adjuvant activity exceeded that of aluminum hydroxide and approached that of Quillaja saponaria-derived saponin formulations. The nanocomplexes also induced balanced cellular immune responses comparable to those reported for Quil A-based nanosystems. These findings identify H. glabra as a promising alternative source of bioactive saponins and demonstrate the potential of plant-derived nanocomplexes as versatile adjuvant platforms for next-generation vaccines.
SN - 1420-3049
ER -