The Molecular Bases of the Interaction between a Saponin from the Roots of Gypsophila paniculata L. and Model Lipid Membranes
Abstract
In view of the possible medical applications of saponins, the molecular structure of a GOTCAB saponin from the roots of Gypsophila paniculata L. was determined by NMR. The biological activity of saponins may depend on the interaction with cell membranes. To obtain more insight in the mechanism of membrane-related saponin function, an experimental and theoretical study was conducted. Ternary lipid systems composed of sphingomyelin, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, and cholesterol were used as models of mammalian cell membranes. The membrane–saponin interaction was studied experimentally by monitoring surface pressure in the monomolecular films formed at the air–aqueous subphase interface. The behavior of GOTCAB saponin in a water box and model monolayer systems was characterized by molecular dynamics simulations. The results obtained showed that, in the systems used, cholesterol had a decisive effect on the interaction between GOTCAB and phosphocholine or sphingomyelin as well as on its location within the lipid film.
Related
- α‐Glucosidase and Acetylcholinesterase (AChE) Inhibitory Activities, Larvicidal Potential and GC–MS Analysis of Endlicheria paniculata: A Combined Experimental and Molecular Docking Study
- Interaction of elastin-derived peptides with model lipid membranes
- Saponins-Containing Plants of the Genera Gypsophila L. and Acanthophyllum C. A. Mey. (Caryophyllaceae) from Southeastern Kazakhstan: Root Morphometry, Saponins Content and Antimicrobial Activity of Extracts
- Exploring the therapeutic potential of Erycibe paniculata (Roxb.): metabolite profiling and antimalarial, anticancer, and modulation of NF-κB signaling pathway for anti-inflammatory activities
- Genetic Relationships, Whole-Fruit Oil Variations, and Quantitative Trait Diversity in Elite Symplocos paniculata Accessions from Hunan Revealed by GBS.
BibTeX
@article{korchowiec2026molecular,
title = {{The Molecular Bases of the Interaction between a Saponin from the Roots of Gypsophila paniculata L. and Model Lipid Membranes}},
author = {Beata Korchowiec and Jacek Korchowiec and Klaudia Kwiecińska and Reneta Gevrenova and Sabine Bouguet-Bonnet and Cheng Deng and Max Henry and Ewa Rogalska},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = sep,
doi = {10.3390/ijms23063397},
url = {https://hal.science/hal-03887468v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - The Molecular Bases of the Interaction between a Saponin from the Roots of Gypsophila paniculata L. and Model Lipid Membranes
AU - Beata Korchowiec
AU - Jacek Korchowiec
AU - Klaudia Kwiecińska
AU - Reneta Gevrenova
AU - Sabine Bouguet-Bonnet
AU - Cheng Deng
AU - Max Henry
AU - Ewa Rogalska
PY - 2026
DA - 2026/09/03
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms23063397
UR - https://hal.science/hal-03887468v1
AB - In view of the possible medical applications of saponins, the molecular structure of a GOTCAB saponin from the roots of Gypsophila paniculata L. was determined by NMR. The biological activity of saponins may depend on the interaction with cell membranes. To obtain more insight in the mechanism of membrane-related saponin function, an experimental and theoretical study was conducted. Ternary lipid systems composed of sphingomyelin, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, and cholesterol were used as models of mammalian cell membranes. The membrane–saponin interaction was studied experimentally by monitoring surface pressure in the monomolecular films formed at the air–aqueous subphase interface. The behavior of GOTCAB saponin in a water box and model monolayer systems was characterized by molecular dynamics simulations. The results obtained showed that, in the systems used, cholesterol had a decisive effect on the interaction between GOTCAB and phosphocholine or sphingomyelin as well as on its location within the lipid film.
SN - 1661-6596
SN - 1422-0067
ER -