Preparation and In Vitro Evaluation of the DOX@Mem-HMnO2 Bioinspired Nanosystem: A Proof-of-Concept Study
- 1Postgraduate Training Base at Shanghai Gongli Hospital, Ningxia Medical University, Shanghai, China
- 2School of Medical Technology, Gongli Hospital, Shanghai University of Technology, Shanghai, China
- 3Department of Orthopedics, Gongli Hospital Pudong New Area, Shanghai, China
Abstract
Introduction: Cell membrane–coated nanoparticles are emerging as promising biomimetic platforms for targeted cancer drug delivery, owing to their homotypic tumor-targeting ability and favorable biocompatibility. In this study, we fabricated a biomimetic DOX@Mem-HMnO2 nanosystem based on hollow mesoporous manganese dioxide (HMnO2) coated with A549 lung adenocarcinoma cell membrane (Mem) and loaded with doxorubicin (DOX), and evaluated its in vitro biological performance using the A549 cell model.
Methods: Monodisperse silica nanoparticles were synthesized via a modified Stöber method as sacrificial templates to prepare hollow mesoporous HMnO2. DOX was loaded into HMnO2, and native A549 cell membrane was isolated by differential centrifugation and wrapped onto DOX-loaded HMnO2. SEM and TEM characterized morphology and membrane coating; UV-Vis spectrophotometry confirmed DOX loading; particle size and zeta potential were measured, and colloidal stability was monitored over 72 h in deionized water and DMEM. CCK-8 and Transwell assays evaluated anti-proliferative and anti-migratory effects (≥3 independent replicates).
Results: DOX@Mem-HMnO2 was successfully prepared as spherical, relatively monodisperse nanoparticles with an observable membrane-like outer layer and retained DOX characteristic absorbance. Zeta potential shifted from −42.1 ± 1.8 mV (bare HMnO2) to −23.8 ± 1.5 mV after membrane coating and −30.2 ± 1.6 mV after DOX loading. The nanosystem remained stable without aggregation over 72 h. All DOX-containing groups inhibited A549 proliferation in a concentration-dependent manner; DOX@Mem-HMnO2 produced significantly stronger anti-proliferative and anti-migratory effects than free DOX and DOX@HMnO2 (P < 0.05), while empty carriers showed low intrinsic cytotoxicity. This study is limited to in vitro A549 experiments; direct evidence for homotypic targeting and in vivo performance is absent.
Conclusion: DOX@Mem-HMnO2 can be readily synthesized with uniform morphology, favorable colloidal stability, and enhanced in vitro anti-proliferative and anti-migratory activity against A549 cells, providing a proof-of-concept basis for further biological validation.
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BibTeX
@article{chen2026preparation,
title = {{Preparation and In Vitro Evaluation of the DOX@Mem-HMnO2 Bioinspired Nanosystem: A Proof-of-Concept Study}},
author = {Jiangang Chen and Ao Xu and Yusheng Tang and Zhen Yang and Shibo Gao and Yan Zhang},
journal = {Technology in Cancer Research \& Treatment},
year = {2026},
month = sep,
doi = {10.1177/15330338261494055},
url = {https://doi.org/10.1177/15330338261494055},
note = {CC BY-NC 4.0},
}
RIS
TY - JOUR
TI - Preparation and In Vitro Evaluation of the DOX@Mem-HMnO2 Bioinspired Nanosystem: A Proof-of-Concept Study
AU - Jiangang Chen
AU - Ao Xu
AU - Yusheng Tang
AU - Zhen Yang
AU - Shibo Gao
AU - Yan Zhang
PY - 2026
DA - 2026/09/29
JO - Technology in Cancer Research & Treatment
DO - 10.1177/15330338261494055
UR - https://doi.org/10.1177/15330338261494055
AB - Introduction: Cell membrane–coated nanoparticles are emerging as promising biomimetic platforms for targeted cancer drug delivery, owing to their homotypic tumor-targeting ability and favorable biocompatibility. In this study, we fabricated a biomimetic DOX@Mem-HMnO2 nanosystem based on hollow mesoporous manganese dioxide (HMnO2) coated with A549 lung adenocarcinoma cell membrane (Mem) and loaded with doxorubicin (DOX), and evaluated its in vitro biological performance using the A549 cell model. Methods: Monodisperse silica nanoparticles were synthesized via a modified Stöber method as sacrificial templates to prepare hollow mesoporous HMnO2. DOX was loaded into HMnO2, and native A549 cell membrane was isolated by differential centrifugation and wrapped onto DOX-loaded HMnO2. SEM and TEM characterized morphology and membrane coating; UV-Vis spectrophotometry confirmed DOX loading; particle size and zeta potential were measured, and colloidal stability was monitored over 72 h in deionized water and DMEM. CCK-8 and Transwell assays evaluated anti-proliferative and anti-migratory effects (≥3 independent replicates). Results: DOX@Mem-HMnO2 was successfully prepared as spherical, relatively monodisperse nanoparticles with an observable membrane-like outer layer and retained DOX characteristic absorbance. Zeta potential shifted from −42.1 ± 1.8 mV (bare HMnO2) to −23.8 ± 1.5 mV after membrane coating and −30.2 ± 1.6 mV after DOX loading. The nanosystem remained stable without aggregation over 72 h. All DOX-containing groups inhibited A549 proliferation in a concentration-dependent manner; DOX@Mem-HMnO2 produced significantly stronger anti-proliferative and anti-migratory effects than free DOX and DOX@HMnO2 (P < 0.05), while empty carriers showed low intrinsic cytotoxicity. This study is limited to in vitro A549 experiments; direct evidence for homotypic targeting and in vivo performance is absent. Conclusion: DOX@Mem-HMnO2 can be readily synthesized with uniform morphology, favorable colloidal stability, and enhanced in vitro anti-proliferative and anti-migratory activity against A549 cells, providing a proof-of-concept basis for further biological validation.
SN - 1533-0346
SN - 1533-0338
ER -