Glyceraldehyde May Induce the Generation of High-Molecular-Weight Species of HSP90β and Autophagy-Related Proteins and Alter Protein Levels in Human Renal Epithelial Cells
7 affiliations
- 1Department of Pathology and Laboratory Medicine, Kanazawa Medical University, Uchinada 920-0293, Ishikawa, Japan
- 2Department of Pathology, Kanazawa Medical University Hospital, Uchinada 920-0293, Ishikawa, Japan
- 3Department of Pathophysiological and Experimental Pathology, Kanazawa Medical University, Uchinada 920-0293, Ishikawa, Japan
- 4Department of Pharmacy, Kanazawa Medical University Hospital, Uchinada 920-0293, Ishikawa, Japan
- 5Department of Urology, Kanazawa Medical University, Uchinada 920-0293, Ishikawa, Japan
- 6Department of Oncogenic Pathology, Kanazawa Medical University, Uchinada 920-0293, Ishikawa, Japan
- 7Department of Oral and Maxillofacial Surgery, Kanazawa Medical University, Uchinada 920-0293, Ishikawa, Japan
Abstract
Kidney disease (KD) is a major health problem, and metabolic disorders contribute to diabetic nephropathy and chronic kidney disease, in which renal epithelial cell dysfunction plays an important role. Intracellular advanced glycation end-products (AGEs) may contribute to KD progression by disrupting cellular homeostasis; however, the underlying mechanisms remain unclear. Therefore, we aimed to investigate the effects of glyceraldehyde (GA) on molecular chaperone- and autophagy-related proteins in human renal epithelial cells (HK-2), with particular attention to AGE-related processes. Administration of high-fructose corn syrup to healthy Wistar rats increased argpyrimidine immunoreactivity in the renal tubular epithelial cells, compared with that in controls, suggesting enhanced accumulation of this GA-related AGE in vivo. In HK-2 cells, GA treatment caused concentration-dependent reductions in NADPH and ATP levels, accompanied by morphological changes. High molecular weight (HMW) immunoreactive species of HSP90β, Beclin-1, and ATG7 appeared following GA treatment. GA at 1.5 mM increased LC3-I, LC3-II, and p62 levels, whereas treatment with 2 mM GA attenuated these increases. Aminoguanidine, an inhibitor of AGE formation, attenuated these GA-induced alterations. Overall, GA may induce the generation of HMW species of HSP90β and autophagy-related proteins and may contribute to altered cellular homeostasis, with AGE-related processes potentially contributing to these effects.
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BibTeX
@article{nguyen2026glyceraldehyde,
title = {{Glyceraldehyde May Induce the Generation of High-Molecular-Weight Species of HSP90β and Autophagy-Related Proteins and Alter Protein Levels in Human Renal Epithelial Cells}},
author = {Thao Thanh Nguyen and Takanobu Takata and Shinya Inoue and Dung Anh Vu and Akihiro Shioya and Miyako Shimasaki and Motona Kumagai and Jia Han and Yao Liu and Wenpeng Jiao and Mitsuaki Yoshida and Yasuhisa Sawai and Hiroyuki Nakano and Togen Masauji and Katsuhito Miyazawa and Sohsuke Yamada},
journal = {Biomolecules},
year = {2026},
month = oct,
doi = {10.3390/biom16101488},
url = {https://www.mdpi.com/2218-273X/16/10/1488},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Glyceraldehyde May Induce the Generation of High-Molecular-Weight Species of HSP90β and Autophagy-Related Proteins and Alter Protein Levels in Human Renal Epithelial Cells
AU - Thao Thanh Nguyen
AU - Takanobu Takata
AU - Shinya Inoue
AU - Dung Anh Vu
AU - Akihiro Shioya
AU - Miyako Shimasaki
AU - Motona Kumagai
AU - Jia Han
AU - Yao Liu
AU - Wenpeng Jiao
AU - Mitsuaki Yoshida
AU - Yasuhisa Sawai
AU - Hiroyuki Nakano
AU - Togen Masauji
AU - Katsuhito Miyazawa
AU - Sohsuke Yamada
PY - 2026
DA - 2026/10/01
JO - Biomolecules
DO - 10.3390/biom16101488
UR - https://www.mdpi.com/2218-273X/16/10/1488
AB - Kidney disease (KD) is a major health problem, and metabolic disorders contribute to diabetic nephropathy and chronic kidney disease, in which renal epithelial cell dysfunction plays an important role. Intracellular advanced glycation end-products (AGEs) may contribute to KD progression by disrupting cellular homeostasis; however, the underlying mechanisms remain unclear. Therefore, we aimed to investigate the effects of glyceraldehyde (GA) on molecular chaperone- and autophagy-related proteins in human renal epithelial cells (HK-2), with particular attention to AGE-related processes. Administration of high-fructose corn syrup to healthy Wistar rats increased argpyrimidine immunoreactivity in the renal tubular epithelial cells, compared with that in controls, suggesting enhanced accumulation of this GA-related AGE in vivo. In HK-2 cells, GA treatment caused concentration-dependent reductions in NADPH and ATP levels, accompanied by morphological changes. High molecular weight (HMW) immunoreactive species of HSP90β, Beclin-1, and ATG7 appeared following GA treatment. GA at 1.5 mM increased LC3-I, LC3-II, and p62 levels, whereas treatment with 2 mM GA attenuated these increases. Aminoguanidine, an inhibitor of AGE formation, attenuated these GA-induced alterations. Overall, GA may induce the generation of HMW species of HSP90β and autophagy-related proteins and may contribute to altered cellular homeostasis, with AGE-related processes potentially contributing to these effects.
SN - 2218-273X
ER -