Recent Advances in Reversed-Phase Liquid Chromatography for Therapeutic Oligonucleotide and Conjugate Characterization: From Ion-Pairing to Green Analytical Approaches.
4 affiliations
- 1Department of Chemical Engineering Vrije Universiteit Brussel (VUB) Brussels Belgium.
- 2Faculty of Chemistry Department of Analytical Chemistry Gdańsk University of Technology Gdańsk Poland.
- 3Therapeutics Development & Supply Janssen Research & Development NV Johnson & Johnson Company Beerse Belgium.
- 4Waters Corporation Global Research Milford Massachusetts USA.
Abstract
Therapeutic oligonucleotides (ONs) have rapidly emerged as a novel class of treatments in the pharmaceutical industry, with over 25 medicines approved in the past decade. Ion-pair reversed-phase liquid chromatography coupled with mass spectrometry (IP-RPLC-MS) is widely used method for ON characterization. This review critically examines the state-of-the-art developments in IP-RPLC-MS for ON analysis, with particular focus on the selection of optimal chromatographic conditions for three key applications: impurity profiling for single-stranded ON, characterization of novel conjugates (e.g., lipid-and peptide-conjugated ONs) and double-stranded siRNA analysis in non-denaturing conditions. Finally, recent developments in ion-pair-free RPLC are discussed in the context of pharmaceutical and bioanalytical applications.
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BibTeX
@article{bui2026recent,
title = {{Recent Advances in Reversed-Phase Liquid Chromatography for Therapeutic Oligonucleotide and Conjugate Characterization: From Ion-Pairing to Green Analytical Approaches.}},
author = {Quang-Dong Bui and Weronika Hewelt-Belka and Tiny Deschrijver and Martin Gilar and Sebastiaan Eeltink},
journal = {Analytical science advances},
year = {2026},
month = oct,
doi = {10.1002/ansa.70113},
url = {https://europepmc.org/article/MED/42948273},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Recent Advances in Reversed-Phase Liquid Chromatography for Therapeutic Oligonucleotide and Conjugate Characterization: From Ion-Pairing to Green Analytical Approaches.
AU - Quang-Dong Bui
AU - Weronika Hewelt-Belka
AU - Tiny Deschrijver
AU - Martin Gilar
AU - Sebastiaan Eeltink
PY - 2026
DA - 2026/10/26
JO - Analytical science advances
DO - 10.1002/ansa.70113
UR - https://europepmc.org/article/MED/42948273
AB - Therapeutic oligonucleotides (ONs) have rapidly emerged as a novel class of treatments in the pharmaceutical industry, with over 25 medicines approved in the past decade. Ion-pair reversed-phase liquid chromatography coupled with mass spectrometry (IP-RPLC-MS) is widely used method for ON characterization. This review critically examines the state-of-the-art developments in IP-RPLC-MS for ON analysis, with particular focus on the selection of optimal chromatographic conditions for three key applications: impurity profiling for single-stranded ON, characterization of novel conjugates (e.g., lipid-and peptide-conjugated ONs) and double-stranded siRNA analysis in non-denaturing conditions. Finally, recent developments in ion-pair-free RPLC are discussed in the context of pharmaceutical and bioanalytical applications.
SN - 2628-5452
ER -