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Peer-reviewedCC BYMLife

Clarification and reprogramming of glucose and xylose metabolism in Sphingomonas sanxanigenens for efficient sanxan biosynthesis.

  • Yue Ming1
  • Zhuangzhuang Shi1
  • Yiting Lin1
  • Jue Li1
  • Weilong Wang1
  • Wei Sha2
  • Guoqiang Li1
  • Mengmeng Wu2
  • Ting Ma1
  1. 1Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences Nankai University Tianjin China.
  2. 2School of Biotechnology and Food Science Tianjin University of Commerce Tianjin China.
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Abstract

Currently, the production of high-value chemicals from natural lignocellulose is of great significance for a circular economy. In this study, a robust platform was developed based on Sphingomonas sanxanigenens NXdP, which co-utilizes glucose and xylose to synthesize polysaccharide sanxan, for efficient bioconversion of lignocellulose-derived sugars. Sanxan is a promising high-value product due to its high viscosity at low concentrations, excellent acid-gelling capabilities, and unique rheological characteristics. Through a cell growth-based screening strategy and gene manipulation, a key transporter (0233) required for the uptake of both glucose and xylose was identified, clarifying the co-utilization mechanism. Additionally, the metabolic pathways of glucose and xylose were individually blocked to assess their contributions and activities. Furthermore, the metabolic network of strain NXdP was reprogrammed via a "flux-redirection" strategy to develop a potential microbial host with improved sanxan production on mixed sugars. The final engineered strain S09 achieved a sanxan titer of 20.25 ± 0.78 g/kg under mixed-sugar fermentation, representing a 13.51% ± 4.38% increase compared with the original strain NXdP. The mechanism underlying this enhanced production was revealed by central carbon metabolism (CCM) analysis. Overall, this study provides insights into the efficient conversion of lignocellulose-derived sugars and offers genetic components for the green production of other high-value-added products.

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Cite

BibTeXRIS
BibTeX
@article{ming2026clarification,
  title = {{Clarification and reprogramming of glucose and xylose metabolism in Sphingomonas sanxanigenens for efficient sanxan biosynthesis.}},
  author = {Yue Ming and Zhuangzhuang Shi and Yiting Lin and Jue Li and Weilong Wang and Wei Sha and Guoqiang Li and Mengmeng Wu and Ting Ma},
  journal = {MLife},
  year = {2026},
  month = oct,
  doi = {10.1002/mlf2.70110},
  url = {https://europepmc.org/article/MED/42909390},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Clarification and reprogramming of glucose and xylose metabolism in Sphingomonas sanxanigenens for efficient sanxan biosynthesis.
AU  - Yue Ming
AU  - Zhuangzhuang Shi
AU  - Yiting Lin
AU  - Jue Li
AU  - Weilong Wang
AU  - Wei Sha
AU  - Guoqiang Li
AU  - Mengmeng Wu
AU  - Ting Ma
PY  - 2026
DA  - 2026/10/21
JO  - MLife
DO  - 10.1002/mlf2.70110
UR  - https://europepmc.org/article/MED/42909390
AB  - Currently, the production of high-value chemicals from natural lignocellulose is of great significance for a circular economy. In this study, a robust platform was developed based on Sphingomonas sanxanigenens NXdP, which co-utilizes glucose and xylose to synthesize polysaccharide sanxan, for efficient bioconversion of lignocellulose-derived sugars. Sanxan is a promising high-value product due to its high viscosity at low concentrations, excellent acid-gelling capabilities, and unique rheological characteristics. Through a cell growth-based screening strategy and gene manipulation, a key transporter (0233) required for the uptake of both glucose and xylose was identified, clarifying the co-utilization mechanism. Additionally, the metabolic pathways of glucose and xylose were individually blocked to assess their contributions and activities. Furthermore, the metabolic network of strain NXdP was reprogrammed via a "flux-redirection" strategy to develop a potential microbial host with improved sanxan production on mixed sugars. The final engineered strain S09 achieved a sanxan titer of 20.25 ± 0.78 g/kg under mixed-sugar fermentation, representing a 13.51% ± 4.38% increase compared with the original strain NXdP. The mechanism underlying this enhanced production was revealed by central carbon metabolism (CCM) analysis. Overall, this study provides insights into the efficient conversion of lignocellulose-derived sugars and offers genetic components for the green production of other high-value-added products.
SN  - 2097-1699
SN  - 2770-100X
ER  -