MR Imaging-based Evaluation of Cerebrospinal Fluid Dynamics in Cynomolgus Monkeys Using ¹⁷O-labeled Saline and Gd-DOTA
Abstract
PURPOSE: O-labeled saline and gadolinium (Gd)-based contrast agents have been applied to investigate CSF dynamics using MRI in preclinical settings, studies in non-human primates remain limited. This study evaluated the feasibility of visualizing CSF kinetics in cynomolgus monkeys using these 2 tracers. METHODS: O-labeled saline protocol, T2-weighted images were acquired every minute for 20 min. For the Gd-DOTA protocol, T1-weighted images were acquired at 10 min, 30 min, 1 hr, 4 hr, and 24 hr after administration. Signal intensities (SIs) within CSF and parenchymal regions were analyzed. RESULTS: O-labeled saline injection, the % change in SI in the cisterna magna peaked rapidly at 2-3 min and subsequently propagated caudally along the cervical spinal canal without detectable parenchymal uptake. In contrast, after Gd-DOTA administration, the % change in SI in the cisterna magna reached its maximum at 1-4 hr, with widespread distribution into ventricles, cisterns, and spinal canal. Gd-DOTA clearance via the cribriform plate was evident. Parenchymal enhancement was observed in the cerebellum, pons, and cingulate gyrus with greater signal change in superficial compared to sub-superficial layers. Marked enhancement was also observed in gray matter surrounding the spinal central canal at 4 hr. CONCLUSION: O-labeled saline enabled rapid visualization of bulk CSF flow, Gd-DOTA provided insights into slower CSF distribution and parenchymal transport. These findings provide a translational platform for future investigations into age- and disease-related alterations in CSF dynamics.
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BibTeX
@article{chatani2026imagingbased,
title = {{MR Imaging-based Evaluation of Cerebrospinal Fluid Dynamics in Cynomolgus Monkeys Using ¹⁷O-labeled Saline and Gd-DOTA}},
author = {Shohei Chatani and Tatsuya Oki and Sayaka Misaki and Neil Roberts and Yoshiyuki Watanabe},
journal = {Magnetic Resonance in Medical Sciences},
year = {2026},
month = oct,
doi = {10.2463/mrms.mp.2026-0048},
url = {https://www.jstage.jst.go.jp/article/mrms/25/4/25_mp.2026-0048/_article},
note = {CC BY},
}
RIS
TY - JOUR
TI - MR Imaging-based Evaluation of Cerebrospinal Fluid Dynamics in Cynomolgus Monkeys Using ¹⁷O-labeled Saline and Gd-DOTA
AU - Shohei Chatani
AU - Tatsuya Oki
AU - Sayaka Misaki
AU - Neil Roberts
AU - Yoshiyuki Watanabe
PY - 2026
DA - 2026/10/23
JO - Magnetic Resonance in Medical Sciences
DO - 10.2463/mrms.mp.2026-0048
UR - https://www.jstage.jst.go.jp/article/mrms/25/4/25_mp.2026-0048/_article
AB - PURPOSE: O-labeled saline and gadolinium (Gd)-based contrast agents have been applied to investigate CSF dynamics using MRI in preclinical settings, studies in non-human primates remain limited. This study evaluated the feasibility of visualizing CSF kinetics in cynomolgus monkeys using these 2 tracers. METHODS: O-labeled saline protocol, T2-weighted images were acquired every minute for 20 min. For the Gd-DOTA protocol, T1-weighted images were acquired at 10 min, 30 min, 1 hr, 4 hr, and 24 hr after administration. Signal intensities (SIs) within CSF and parenchymal regions were analyzed. RESULTS: O-labeled saline injection, the % change in SI in the cisterna magna peaked rapidly at 2-3 min and subsequently propagated caudally along the cervical spinal canal without detectable parenchymal uptake. In contrast, after Gd-DOTA administration, the % change in SI in the cisterna magna reached its maximum at 1-4 hr, with widespread distribution into ventricles, cisterns, and spinal canal. Gd-DOTA clearance via the cribriform plate was evident. Parenchymal enhancement was observed in the cerebellum, pons, and cingulate gyrus with greater signal change in superficial compared to sub-superficial layers. Marked enhancement was also observed in gray matter surrounding the spinal central canal at 4 hr. CONCLUSION: O-labeled saline enabled rapid visualization of bulk CSF flow, Gd-DOTA provided insights into slower CSF distribution and parenchymal transport. These findings provide a translational platform for future investigations into age- and disease-related alterations in CSF dynamics.
SN - 1880-2206
SN - 1347-3182
ER -