Peer-reviewedCC BY 4.0Journal of Integrative Plant Biology23 Jun 2026

Smart double‐screening system of propagating male‐sterile lines for maize hybrid seed production

Ruidan Liu¹
Shengxi Hou¹
Hui Guo¹
Yang Cui¹
Weibin Song^1,2,3
Jie Yang⁴
Hainan Zhao^1,2,3
Qiujie Liu^1,2,3
Jian Chen^1,2,3
Jinsheng Lai^1,2,3
Haiming Zhao^1,2,3

4 affiliations

¹State Key Laboratory of Maize Bio‐breeding, College of Agronomy and Biotechnology China Agricultural University Beijing 100193 China
²Sanya Institute of China Agricultural University Sanya 572025 China
³Frontiers Science Center for Molecular Design Breeding China Agricultural University Beijing 100193 China
⁴Institute of Crop Research Academy of Agricultural Sciences of Xinjiang Uyghur Autonomous Region Urumqi 830091 China

Abstract

The Seed and Seedling Marker Method double‐screening system for maize hybrid seed production integrates Ms45‐mediated fertility restoration, Mn1/Sh1‐RNAi‐based seed selection, and Lc‐based seedling selection. It thus enables efficient, high‐purity propagation of male‐sterile lines by combining fertility maintenance with sequential visual selection at the seed and seedling stages.

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BibTeX RIS

BibTeX

@article{liu2026smart,
  title = {{Smart double‐screening system of propagating male‐sterile lines for maize hybrid seed production}},
  author = {Ruidan Liu and Shengxi Hou and Hui Guo and Yang Cui and Weibin Song and Jie Yang and Hainan Zhao and Qiujie Liu and Jian Chen and Jinsheng Lai and Haiming Zhao},
  journal = {Journal of Integrative Plant Biology},
  year = {2026},
  month = jun,
  doi = {10.1111/jipb.70331},
  url = {https://doi.org/10.1111/jipb.70331},
  note = {CC BY 4.0},
}

RIS

TY  - JOUR
TI  - Smart double‐screening system of propagating male‐sterile lines for maize hybrid seed production
AU  - Ruidan Liu
AU  - Shengxi Hou
AU  - Hui Guo
AU  - Yang Cui
AU  - Weibin Song
AU  - Jie Yang
AU  - Hainan Zhao
AU  - Qiujie Liu
AU  - Jian Chen
AU  - Jinsheng Lai
AU  - Haiming Zhao
PY  - 2026
DA  - 2026/06/23
JO  - Journal of Integrative Plant Biology
DO  - 10.1111/jipb.70331
UR  - https://doi.org/10.1111/jipb.70331
AB  - The Seed and Seedling Marker Method double‐screening system for maize hybrid seed production integrates Ms45‐mediated fertility restoration, Mn1/Sh1‐RNAi‐based seed selection, and Lc‐based seedling selection. It thus enables efficient, high‐purity propagation of male‐sterile lines by combining fertility maintenance with sequential visual selection at the seed and seedling stages.
SN  - 1672-9072
SN  - 1744-7909
ER  -

Abstract

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