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Peer-reviewedCC BYSensors and Actuators Reports

Preamplification-free detection of

  • Wei Wang1
  • Li Zhao2
  • Peng Shu3
  • Yao Wu1
  • Hangtian Zhou1
  • Zhaohui Qiao4
  • Weisheng Cheng5
5 affiliations
  1. 1Department of Pathology, the First Affiliated Hospital of Anhui Medical University, Hefei, 230001, China
  2. 2High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Anhui Province Key Laboratory of Environmental Toxicology and Pollution Control Technology, Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Chinese Academy of Sciences, Hefei, Anhui, 230031, China
  3. 3Precision Medicine Research Centre, Beilun People’s Hospital, Ningbo, 315800, China
  4. 4Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, College of Food Science and Engineering, Ningbo University, Ningbo, 315800, China; Corresponding authors.
  5. 5Prenatal Diagnosis Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei, Anhui, 230001, China; Corresponding authors.
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Abstract

Rapid and sensitive colorimetric detection of Salmonella in food samples remains challenging. Herein, we developed a preamplification-free colorimetric platform for sensitive detection of Salmonella by integrating whole-cell bio-barcode immunoassay with a Cas12a-gated HCR/G-quadruplex DNAzyme cascade. In this system, target bacteria were immunomagnetically separated and labeled with barcode DNA-modified AuNPs, which converted bacterial cells into abundant Cas12a-activating DNA signals. The activated Cas12a subsequently regulated the HCR/G-quadruplex DNAzyme system, leading to a target-dependent colorimetric response. Benefiting from efficient whole-cell enrichment, bio-barcode signal transduction, and enzyme-free DNAzyme amplification, the proposed method enabled sensitive colorimetric detection of Salmonella with a detection limit of 13 CFU mL −1 and a practical linear range of 10 2 –10 6 CFU mL −1 . Moreover, the platform exhibited good specificity and was successfully applied to Salmonella detection in artificially spiked milk and chicken samples, supporting its feasibility in complex food matrices. Owing to the programmability of barcode DNA and CRISPR/Cas12a, this strategy provides a preamplification-free route for colorimetric detection of foodborne pathogens.

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Cite

BibTeXRIS
BibTeX
@article{wang2026preamplificationfree,
  title = {{Preamplification-free detection of}},
  author = {Wei Wang and Li Zhao and Peng Shu and Yao Wu and Hangtian Zhou and Zhaohui Qiao and Weisheng Cheng},
  journal = {Sensors and Actuators Reports},
  year = {2026},
  month = oct,
  doi = {10.1016/j.snr.2026.100520},
  url = {https://doi.org/10.1016/j.snr.2026.100520},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Preamplification-free detection of
AU  - Wei Wang
AU  - Li Zhao
AU  - Peng Shu
AU  - Yao Wu
AU  - Hangtian Zhou
AU  - Zhaohui Qiao
AU  - Weisheng Cheng
PY  - 2026
DA  - 2026/10/30
JO  - Sensors and Actuators Reports
DO  - 10.1016/j.snr.2026.100520
UR  - https://doi.org/10.1016/j.snr.2026.100520
AB  - Rapid and sensitive colorimetric detection of Salmonella in food samples remains challenging. Herein, we developed a preamplification-free colorimetric platform for sensitive detection of Salmonella by integrating whole-cell bio-barcode immunoassay with a Cas12a-gated HCR/G-quadruplex DNAzyme cascade. In this system, target bacteria were immunomagnetically separated and labeled with barcode DNA-modified AuNPs, which converted bacterial cells into abundant Cas12a-activating DNA signals. The activated Cas12a subsequently regulated the HCR/G-quadruplex DNAzyme system, leading to a target-dependent colorimetric response. Benefiting from efficient whole-cell enrichment, bio-barcode signal transduction, and enzyme-free DNAzyme amplification, the proposed method enabled sensitive colorimetric detection of Salmonella with a detection limit of 13 CFU mL −1 and a practical linear range of 10 2 –10 6 CFU mL −1 . Moreover, the platform exhibited good specificity and was successfully applied to Salmonella detection in artificially spiked milk and chicken samples, supporting its feasibility in complex food matrices. Owing to the programmability of barcode DNA and CRISPR/Cas12a, this strategy provides a preamplification-free route for colorimetric detection of foodborne pathogens.
SN  - 2666-0539
ER  -