DIRECTION-AWARE INTEGRATED BIOINFORMATICS ANALYSIS REVEALS CONCORDANT AND DISCORDANT MOLECULAR SIGNATURES LINKING TYPE 2 DIABETES MELLITUS AND POLYCYSTIC OVARY SYNDROME
- 1University of Ibadan
Abstract
Polycystic ovarian syndrome and Type 2 diabetes mellitus are complex multivariate diseases that share well-established clinical pathophysiology, yet the molecular basis of their overlap is unknown. Prior bioinformatics studies have identified common differentially expressed genes between these two conditions. However, they have not accounted for the directionality of the gene expression changes observed, potentially obscuring any biological distinctions they may have. This study applied a direction-aware approach to classify molecular signatures between PCOS and T2DM as concordant or discordant. Transcriptomic analysis of GEO datasets GSE138518 (ovarian granulosa tissue, PCOS) and GSE25724 (pancreatic islet tissue, T2DM) identified 225 and 1,302 DEGs, respectively. Venn diagram analysis showed that only three genes (SLC6A8, RGS4, and SORL1) were shared between PCOS and T2DM from the total of 1,527 genes, and all 3 genes were regulated in opposing directions. Disease gene retrieval from the Comparative Toxicogenomics Database, Online Mendelian Inheritance in Man database, and GeneCards showed 214 shared disease-associated genes, with 311 genes unique to PCOS and 496 genes unique to T2DM. Protein-protein interaction construction using STRING (version 12.0) identified 70 interacting nodes. CytoHubba analysis across 6 scoring methods identified 18 high-confidence hub genes, including INS, BCL2, MTOR, LEP, MFN2, and PIK3CD. Functional enrichment analysis identified biological processes including immune activation, apoptosis, and insulin signaling, which were confirmed as the main pathways in KEGG pathway analysis. These findings show that while PCOS and T2DM share limited DEGs, they share common pathogenic pathways.
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BibTeX
@misc{abdulsalam2026directionaware,
title = {{DIRECTION-AWARE INTEGRATED BIOINFORMATICS ANALYSIS REVEALS CONCORDANT AND DISCORDANT MOLECULAR SIGNATURES LINKING TYPE 2 DIABETES MELLITUS AND POLYCYSTIC OVARY SYNDROME}},
author = {s. O. Abdulsalam and D. A. Akanbi and T. E. Aloba and M. W. Ogunlola and D. I. Owopetu},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.20.753005},
url = {https://www.biorxiv.org/content/10.64898/2026.09.20.753005v1},
note = {CC BY-NC},
}
RIS
TY - UNPB
TI - DIRECTION-AWARE INTEGRATED BIOINFORMATICS ANALYSIS REVEALS CONCORDANT AND DISCORDANT MOLECULAR SIGNATURES LINKING TYPE 2 DIABETES MELLITUS AND POLYCYSTIC OVARY SYNDROME
AU - s. O. Abdulsalam
AU - D. A. Akanbi
AU - T. E. Aloba
AU - M. W. Ogunlola
AU - D. I. Owopetu
PY - 2026
DA - 2026/09/20
PB - bioRxiv
DO - 10.64898/2026.09.20.753005
UR - https://www.biorxiv.org/content/10.64898/2026.09.20.753005v1
AB - Polycystic ovarian syndrome and Type 2 diabetes mellitus are complex multivariate diseases that share well-established clinical pathophysiology, yet the molecular basis of their overlap is unknown. Prior bioinformatics studies have identified common differentially expressed genes between these two conditions. However, they have not accounted for the directionality of the gene expression changes observed, potentially obscuring any biological distinctions they may have. This study applied a direction-aware approach to classify molecular signatures between PCOS and T2DM as concordant or discordant. Transcriptomic analysis of GEO datasets GSE138518 (ovarian granulosa tissue, PCOS) and GSE25724 (pancreatic islet tissue, T2DM) identified 225 and 1,302 DEGs, respectively. Venn diagram analysis showed that only three genes (SLC6A8, RGS4, and SORL1) were shared between PCOS and T2DM from the total of 1,527 genes, and all 3 genes were regulated in opposing directions. Disease gene retrieval from the Comparative Toxicogenomics Database, Online Mendelian Inheritance in Man database, and GeneCards showed 214 shared disease-associated genes, with 311 genes unique to PCOS and 496 genes unique to T2DM. Protein-protein interaction construction using STRING (version 12.0) identified 70 interacting nodes. CytoHubba analysis across 6 scoring methods identified 18 high-confidence hub genes, including INS, BCL2, MTOR, LEP, MFN2, and PIK3CD. Functional enrichment analysis identified biological processes including immune activation, apoptosis, and insulin signaling, which were confirmed as the main pathways in KEGG pathway analysis. These findings show that while PCOS and T2DM share limited DEGs, they share common pathogenic pathways.
ER -