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Peer-reviewedCC BY 4.0Cells

Transient Receptor Potential Ankyrin 1 and Vanilloid 1 Receptor mRNAs Are Not Translated to Functional Ion Channels in Human Prostate Adenocarcinoma Cells

  • Anita Steib1,2
  • Katalin Rozmer1,2,3
  • Zoltán Sándor1,2
  • Éva Szőke1,2,4
  • Dávid Semjén5
  • Béla Kajtár5
  • Krisztina Pohóczky1,2,6
  • Zsuzsanna Helyes1,2,4,7
7 affiliations
  1. 1Department of Pharmacology and Pharmacotherapy, Medical School, University of Pécs, 7624 Pécs, Hungary
  2. 2National Laboratory for Drug Research and Development (PharmaLab), 1117 Budapest, Hungary
  3. 3Institute of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Pécs, 7624 Pécs, Hungary
  4. 4Chronic Pain Research Group (HUN-REN PTE), Hungarian Research Network, 7624 Pécs, Hungary
  5. 5Department of Pathology, Medical School, University of Pécs, 7624 Pécs, Hungary
  6. 6Department of Pharmacology, Faculty of Pharmacy, University of Pécs, 7624 Pécs, Hungary
  7. 7PharmInVivo Ltd., 7629 Pécs, Hungary
Open at sourceDOICite

Abstract

Transient receptor potential ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) have been implicated in cancer biology, but their functional relevance in prostate adenocarcinoma (PAC) remains unclear. Therefore, we characterized TRPA1/TRPV1 expression and function in benign prostatic hyperplasia (BPH), PAC tissues, and PAC cell lines with different characteristics and examined the effects of their agonists on cancer-cell viability. TRPA1 and TRPV1 transcripts were assessed by RNAscope in situ hybridization and RT-qPCR, channel activity by Fura-2-based Ca2+ imaging, and viability/proliferation by ATP-based luminescence and automated cell counting. TRPA1 and/or TRPV1 mRNAs were detected in PAC tissues and cell lines, but selective stimulation with allyl-isothiocyanate (AITC) or capsaicin did not evoke Ca2+ influx, indicating no detectable functional channel activity under our experimental conditions. Non-selective TRPA1 activators induced Ca2+ influx responses that were not inhibited by the receptor antagonist A-967079. AITC concentration dependently reduced viability and proliferation of the androgen-sensitive 22Rv1 and LNCaP cells, and these effects were not prevented by TRPA1 antagonists. Capsaicin had no effect at TRPV1-selective concentrations. Thus, transcript expression does not predict functional TRPA1/TRPV1 activity in PAC cells, and AITC reduces PAC-cell viability through TRPA1-independent mechanisms.

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Cite

BibTeXRIS
BibTeX
@article{steib2026transient,
  title = {{Transient Receptor Potential Ankyrin 1 and Vanilloid 1 Receptor mRNAs Are Not Translated to Functional Ion Channels in Human Prostate Adenocarcinoma Cells}},
  author = {Anita Steib and Katalin Rozmer and Zoltán Sándor and Éva Szőke and Dávid Semjén and Béla Kajtár and Krisztina Pohóczky and Zsuzsanna Helyes},
  journal = {Cells},
  year = {2026},
  month = oct,
  doi = {10.3390/cells15211986},
  url = {https://www.mdpi.com/2073-4409/15/21/1986},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Transient Receptor Potential Ankyrin 1 and Vanilloid 1 Receptor mRNAs Are Not Translated to Functional Ion Channels in Human Prostate Adenocarcinoma Cells
AU  - Anita Steib
AU  - Katalin Rozmer
AU  - Zoltán Sándor
AU  - Éva Szőke
AU  - Dávid Semjén
AU  - Béla Kajtár
AU  - Krisztina Pohóczky
AU  - Zsuzsanna Helyes
PY  - 2026
DA  - 2026/10/30
JO  - Cells
DO  - 10.3390/cells15211986
UR  - https://www.mdpi.com/2073-4409/15/21/1986
AB  - Transient receptor potential ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) have been implicated in cancer biology, but their functional relevance in prostate adenocarcinoma (PAC) remains unclear. Therefore, we characterized TRPA1/TRPV1 expression and function in benign prostatic hyperplasia (BPH), PAC tissues, and PAC cell lines with different characteristics and examined the effects of their agonists on cancer-cell viability. TRPA1 and TRPV1 transcripts were assessed by RNAscope in situ hybridization and RT-qPCR, channel activity by Fura-2-based Ca2+ imaging, and viability/proliferation by ATP-based luminescence and automated cell counting. TRPA1 and/or TRPV1 mRNAs were detected in PAC tissues and cell lines, but selective stimulation with allyl-isothiocyanate (AITC) or capsaicin did not evoke Ca2+ influx, indicating no detectable functional channel activity under our experimental conditions. Non-selective TRPA1 activators induced Ca2+ influx responses that were not inhibited by the receptor antagonist A-967079. AITC concentration dependently reduced viability and proliferation of the androgen-sensitive 22Rv1 and LNCaP cells, and these effects were not prevented by TRPA1 antagonists. Capsaicin had no effect at TRPV1-selective concentrations. Thus, transcript expression does not predict functional TRPA1/TRPV1 activity in PAC cells, and AITC reduces PAC-cell viability through TRPA1-independent mechanisms.
SN  - 2073-4409
ER  -