Preoperative Serum Transient Receptor Potential Ankyrin 1 (TRPA1) Levels in Class III Obesity: A Prospective Controlled Observational Study
5 affiliations
- 1Department of General Surgery, University of Health Sciences, Elazığ Fethi Sekin City Hospital, Elazığ 23300, Türkiye
- 2Department of General Surgery, Elazığ Fethi Sekin City Hospital, Elazığ 23300, Türkiye
- 3Department of General Surgery, Malatya Training and Research Hospital, Malatya 44000, Türkiye
- 4Department of Biochemistry, Elazığ Fethi Sekin City Hospital, Elazığ 23300, Türkiye
- 5Department of General Surgery, Special Kayseri Dünyam Hospital, Kayseri 38010, Türkiye
Abstract
Transient Receptor Potential Ankyrin 1 (TRPA1) is a membrane cation channel activated by oxidative stress products that are chronically elevated in class III obesity. Although tissue-level TRPA1 biology is increasingly well described, the biological meaning of circulating immunoreactive TRPA1 remains poorly established. This single-centre, prospective, controlled observational study compared preoperative serum TRPA1 between adults with class III obesity scheduled for laparoscopic sleeve gastrectomy and contemporaneous controls, and explored metabolic and inflammatory correlates of the circulating signal. Serum TRPA1 was quantified by enzyme-linked immunosorbent assay (ELISA) in 42 consecutive surgical candidates and 36 controls. The primary analysis used continuous (log-transformed) TRPA1 with age- and sex-adjusted logistic regression; ROC-derived dichotomisation was retained only as an exploratory analysis. Serum TRPA1 did not differ between groups (median 309.8 vs. 340.5 pg/mL; Mann–Whitney U, p = 0.323; Hodges–Lehmann median difference [obesity − control] −30.8 pg/mL, bootstrap 95% CI −227.43 to 43.02). After adjustment for age and sex, continuous log-TRPA1 was not independently associated with obesity status (OR per log-unit 0.88, 95% CI 0.46–1.69; p = 0.699), and discrimination was poor (AUC 0.435; 95% CI 0.305–0.562). Established inflammatory and metabolic markers differed between groups, but serum TRPA1 did not track them. In this cohort, preoperative serum TRPA1 did not distinguish class III obesity from controls. The negative circulating finding should be interpreted as lack of evidence for a large between-group difference in ELISA-measured serum TRPA1—and as a potential translational disconnect from tissue TRPA1 biology—rather than as the definitive exclusion of any tissue-level role for the channel.
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BibTeX
@article{kutluer2026preoperative,
title = {{Preoperative Serum Transient Receptor Potential Ankyrin 1 (TRPA1) Levels in Class III Obesity: A Prospective Controlled Observational Study}},
author = {Nizamettin Kutluer and Onur Ağ and Tamer Gündoğdu and Mehmet Zeki Öğüt and Denizhan Kılıç and Hakan Ayyıldız and Serhat Doğan and Mehmet Buğra Bozan},
journal = {International journal of molecular sciences},
year = {2026},
month = oct,
doi = {10.3390/ijms27198919},
url = {https://europepmc.org/article/PMC/PMC13665438},
note = {CC BY},
}
RIS
TY - JOUR
TI - Preoperative Serum Transient Receptor Potential Ankyrin 1 (TRPA1) Levels in Class III Obesity: A Prospective Controlled Observational Study
AU - Nizamettin Kutluer
AU - Onur Ağ
AU - Tamer Gündoğdu
AU - Mehmet Zeki Öğüt
AU - Denizhan Kılıç
AU - Hakan Ayyıldız
AU - Serhat Doğan
AU - Mehmet Buğra Bozan
PY - 2026
DA - 2026/10/01
JO - International journal of molecular sciences
DO - 10.3390/ijms27198919
UR - https://europepmc.org/article/PMC/PMC13665438
AB - Transient Receptor Potential Ankyrin 1 (TRPA1) is a membrane cation channel activated by oxidative stress products that are chronically elevated in class III obesity. Although tissue-level TRPA1 biology is increasingly well described, the biological meaning of circulating immunoreactive TRPA1 remains poorly established. This single-centre, prospective, controlled observational study compared preoperative serum TRPA1 between adults with class III obesity scheduled for laparoscopic sleeve gastrectomy and contemporaneous controls, and explored metabolic and inflammatory correlates of the circulating signal. Serum TRPA1 was quantified by enzyme-linked immunosorbent assay (ELISA) in 42 consecutive surgical candidates and 36 controls. The primary analysis used continuous (log-transformed) TRPA1 with age- and sex-adjusted logistic regression; ROC-derived dichotomisation was retained only as an exploratory analysis. Serum TRPA1 did not differ between groups (median 309.8 vs. 340.5 pg/mL; Mann–Whitney U, p = 0.323; Hodges–Lehmann median difference [obesity − control] −30.8 pg/mL, bootstrap 95% CI −227.43 to 43.02). After adjustment for age and sex, continuous log-TRPA1 was not independently associated with obesity status (OR per log-unit 0.88, 95% CI 0.46–1.69; p = 0.699), and discrimination was poor (AUC 0.435; 95% CI 0.305–0.562). Established inflammatory and metabolic markers differed between groups, but serum TRPA1 did not track them. In this cohort, preoperative serum TRPA1 did not distinguish class III obesity from controls. The negative circulating finding should be interpreted as lack of evidence for a large between-group difference in ELISA-measured serum TRPA1—and as a potential translational disconnect from tissue TRPA1 biology—rather than as the definitive exclusion of any tissue-level role for the channel.
SN - 1422-0067
ER -