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Peer-reviewedCC BY-NC-NDPhotosynthetica

Quantification of spatial heterogeneity of different chlorophyll fluorescence parameters over tomato leaf areas affected by Cd²⁺ stress.

  • D Giordano1,2
  • A Saadati3,2
  • A M Ashrafi4
  • L Richtera5
  • M Barták1
5 affiliations
  1. 1Laboratory of Photosynthetic Processes, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
  2. 2These authors contributed equally to this work.
  3. 3Central European Institute of Technology, Brno University of Technology, Purkyňova 123, 612 00 Brno, Czech Republic.
  4. 4Institute of Photonics and Electronics of the Czech Academy of Sciences, Chaberská 57, 182 00 Prague 8, Czech Republic.
  5. 5Department of Chemistry and Biochemistry, Mendel University in Brno, Zemědělská 1665/1, 613 00 Brno, Czech Republic.
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Abstract

Cadmium impairs plant growth by inducing oxidative damage through excessive ROS production. Sulfur and nitrogen supplementation can affect Cd mobility, uptake, and toxicity. This study quantifies the spatial heterogeneity of Cd-induced photochemical limitation in Solanum lycopersicum leaves using chlorophyll fluorescence imaging, and examines Cd²⁺ distribution in roots, stems, and leaves of hydroponic plants treated with ammonium sulfate. We assessed four treatments: control, ammonium sulfate (5 mM), cadmium (100 µM CdCl₂), and their combination, using fluorescence imaging, the JIP-test, and polarography. Cd stress was intrinsically heterogeneous within single leaves, with the strongest PSII inhibition along vascular tissue and leaf tips. Contrary to expectations, ammonium sulfate failed to mitigate Cd toxicity, suggesting an adverse ammonium-Cd interaction. OJIP analysis revealed severe oxygen-evolving complex damage under combined treatment, marked by prominent K- and L-bands. These findings highlight the localised nature of Cd stress and reveal responses missed by whole-leaf measurements.

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Cite

BibTeXRIS
BibTeX
@article{giordano2026quantification,
  title = {{Quantification of spatial heterogeneity of different chlorophyll fluorescence parameters over tomato leaf areas affected by Cd²⁺ stress.}},
  author = {D Giordano and A Saadati and A M Ashrafi and L Richtera and M Barták},
  journal = {Photosynthetica},
  year = {2026},
  month = oct,
  doi = {10.32615/ps.2026.035},
  url = {https://europepmc.org/article/MED/42973980},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - Quantification of spatial heterogeneity of different chlorophyll fluorescence parameters over tomato leaf areas affected by Cd²⁺ stress.
AU  - D Giordano
AU  - A Saadati
AU  - A M Ashrafi
AU  - L Richtera
AU  - M Barták
PY  - 2026
DA  - 2026/10/15
JO  - Photosynthetica
DO  - 10.32615/ps.2026.035
UR  - https://europepmc.org/article/MED/42973980
AB  - Cadmium impairs plant growth by inducing oxidative damage through excessive ROS production. Sulfur and nitrogen supplementation can affect Cd mobility, uptake, and toxicity. This study quantifies the spatial heterogeneity of Cd-induced photochemical limitation in Solanum lycopersicum leaves using chlorophyll fluorescence imaging, and examines Cd²⁺ distribution in roots, stems, and leaves of hydroponic plants treated with ammonium sulfate. We assessed four treatments: control, ammonium sulfate (5 mM), cadmium (100 µM CdCl₂), and their combination, using fluorescence imaging, the JIP-test, and polarography. Cd stress was intrinsically heterogeneous within single leaves, with the strongest PSII inhibition along vascular tissue and leaf tips. Contrary to expectations, ammonium sulfate failed to mitigate Cd toxicity, suggesting an adverse ammonium-Cd interaction. OJIP analysis revealed severe oxygen-evolving complex damage under combined treatment, marked by prominent K- and L-bands. These findings highlight the localised nature of Cd stress and reveal responses missed by whole-leaf measurements.
SN  - 0300-3604
SN  - 1573-9058
ER  -