Tissue-specific variability in protein expression of cytochrome P450 and glutathione S-transferase isoenzymes in non-small cell lung carcinoma.
7 affiliations
- 1Ankara University Vocational School of Health Services, Department of Pharmacy Services, Ankara, Turkey.
- 2Kırıkkale University Faculty of Arts and Sciences, Department of Biology, Kırıkkale, Turkey.
- 3Yıldız Technical University Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, İstanbul, Turkey.
- 4University of Health Sciences, Ankara Atatürk Sanatorium Training and Research Hospital, Department of Pathology, Ankara, Turkey.
- 5University of Health Sciences, Ankara Atatürk Sanatorium Training and Research Hospital, Department of Thoracic Surgery, Ankara, Turkey.
- 6Cyprus International University Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Nicosia, Northern Cyprus.
- 7Ankara University Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Ankara, Turkey.
Abstract
Earlier tissue-based studies report heterogeneous CYP and GST protein expression in non-small cell lung cancer (NSCLC), but simultaneous assessment of phase I and II enzymes in paired tumour and peripheral normal tissues remains limited and their expression patterns poorly characterised across the two major NSCLC histological subtypes, namely adenocarcinoma (AC) and squamous cell carcinoma (SCC). To address these gaps, this retrospective study evaluated CYP1A1, CYP1B1, CYP2E1, GSTM1, GSTT1, and GSTP1 expression immunohistochemically in tumour and peripheral normal lung tissues of 50 patients with NSCLC, 27 of whom with AC and 23 with SCC. Cytoplasmic staining intensity was scored from 0 to 3. Tissue and subtype comparisons were performed using the Mann-Whitney U test, and associations with clinical variables were assessed using Spearman's rank correlation. CYP2E1 expression was significantly higher in tumour than in normal tissue in the combined NSCLC group and in both histological subtypes (p<0.05). CYP1A1 expression was higher in AC tumours than in normal tissue and SCC tumours (both p<0.05). GSTM1 expression was higher in tumour tissue in the combined NSCLC group and in the SCC subgroup and was also higher in SCC than in AC tumours (p<0.05). GSTT1 expression was higher in AC than SCC tumours (p<0.05). CYP1B1 and GSTP1 were highly expressed in both tissue compartments but showed no significant tumour-specific increase. CYP and GST isoenzymes therefore showed tissue- and subtype-dependent rather than uniform expression patterns in NSCLC. The differences observed in CYP2E1, CYP1A1, GSTM1, and GSTT1 suggest that AC and SCC may differ in xenobiotic metabolism and cellular defence, and that these isoenzymes may contribute to metabolic processes associated with NSCLC pathogenesis.
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BibTeX
@article{kılıc2026tissuespecific,
title = {{Tissue-specific variability in protein expression of cytochrome P450 and glutathione S-transferase isoenzymes in non-small cell lung carcinoma.}},
author = {Murat Kılıç and Serpil Oğuztüzün and Sezgin Çelik and Funda Demirağ and Pınar Bıçakçıoğlu and Mümtaz İşcan and Ahmet Oğuz Ada},
journal = {Arhiv za higijenu rada i toksikologiju},
year = {2026},
month = sep,
doi = {10.2478/aiht-2026-77-4112},
url = {https://europepmc.org/article/MED/42859408},
note = {CC BY},
}
RIS
TY - JOUR
TI - Tissue-specific variability in protein expression of cytochrome P450 and glutathione S-transferase isoenzymes in non-small cell lung carcinoma.
AU - Murat Kılıç
AU - Serpil Oğuztüzün
AU - Sezgin Çelik
AU - Funda Demirağ
AU - Pınar Bıçakçıoğlu
AU - Mümtaz İşcan
AU - Ahmet Oğuz Ada
PY - 2026
DA - 2026/09/25
JO - Arhiv za higijenu rada i toksikologiju
DO - 10.2478/aiht-2026-77-4112
UR - https://europepmc.org/article/MED/42859408
AB - Earlier tissue-based studies report heterogeneous CYP and GST protein expression in non-small cell lung cancer (NSCLC), but simultaneous assessment of phase I and II enzymes in paired tumour and peripheral normal tissues remains limited and their expression patterns poorly characterised across the two major NSCLC histological subtypes, namely adenocarcinoma (AC) and squamous cell carcinoma (SCC). To address these gaps, this retrospective study evaluated CYP1A1, CYP1B1, CYP2E1, GSTM1, GSTT1, and GSTP1 expression immunohistochemically in tumour and peripheral normal lung tissues of 50 patients with NSCLC, 27 of whom with AC and 23 with SCC. Cytoplasmic staining intensity was scored from 0 to 3. Tissue and subtype comparisons were performed using the Mann-Whitney U test, and associations with clinical variables were assessed using Spearman's rank correlation. CYP2E1 expression was significantly higher in tumour than in normal tissue in the combined NSCLC group and in both histological subtypes (p<0.05). CYP1A1 expression was higher in AC tumours than in normal tissue and SCC tumours (both p<0.05). GSTM1 expression was higher in tumour tissue in the combined NSCLC group and in the SCC subgroup and was also higher in SCC than in AC tumours (p<0.05). GSTT1 expression was higher in AC than SCC tumours (p<0.05). CYP1B1 and GSTP1 were highly expressed in both tissue compartments but showed no significant tumour-specific increase. CYP and GST isoenzymes therefore showed tissue- and subtype-dependent rather than uniform expression patterns in NSCLC. The differences observed in CYP2E1, CYP1A1, GSTM1, and GSTT1 suggest that AC and SCC may differ in xenobiotic metabolism and cellular defence, and that these isoenzymes may contribute to metabolic processes associated with NSCLC pathogenesis.
SN - 0004-1254
SN - 1848-6312
ER -