Distinct lipid and hormonal signatures reveal divergent responses to Erysiphe necator in resistant and susceptible grapevine genotypes.
6 affiliations
- 1C3A - Center Agriculture Food Environment, University of Trento, Via Edmund Mach 1, San Michele all'Adige, 38098, Italy.
- 2Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, San Michele all'Adige, 38098, Italy.
- 3Department of Sustainable Crop Production, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, Piacenza, 29122, Italy.
- 4Research Group Plant Defense Physiology, Max-Planck-Institute for Chemical Ecology, Hans-Knöll-Str. 8, Jena, D-07745, Germany.
- 5Department of Physics, University of Trento, Via Sommarive 14, Povo, 38123, Italy.
- 6Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, San Michele all'Adige, 38098, Italy. [email protected].
Abstract
Background
Powdery mildew, caused by the obligate biotroph Erysiphe necator, represents a major threat to grapevine production worldwide. Host-mediated resistance offers a sustainable alternative to chemical fungicides. To elucidate the role of plant membrane lipid modifications and oxylipin accumulation in pathogen perception and response, a controlled infection experiment was conducted comparing a resistant hybrid (NY_39) from the Edmund Mach Foundation germplasm collection with a susceptible variety (cv. 'Teroldego').Results
A lipidomic approach was integrated with hormone profiling and lipoxygenase (LOX) gene expression analysis. Significant lipid modulation was observed following E. necator inoculation, highlighting the plasticity of membrane and storage lipids metabolism. A rapid decrease in triacylglycerol content was measured in NY_39 at 12 h post-inoculation (hpi), whereas the opposite trend occurred in 'Teroldego', suggesting divergent metabolic rearrangements. A fast galactolipids peroxidation occurred in NY_39, while a delayed accumulation of phosphatidic acid was observed in 'Teroldego' at 48 hpi. The resistant genotype exhibited higher constitutive levels of salicylic acid as well as the expression of the VviAMP1 defensin, a small cysteine-rich protein with broad-spectrum antifungal activity. VviPR10-s11 and VviWRKY51, related to the synthesis of lignin and stilbenoid phytoalexins, were induced at 12 hpi in both genotypes. Within the LOX family, significant up-regulation of the 9-LOX VviLOX1a and the 13-LOXs VviLOX9 and VviLOXO was specific to 'Teroldego' at 12 hpi, despite a higher constitutive level of VviLOX9 in NY_39.Conclusions
This work identifies key metabolic, signalling and gene expression features in the selected hybrid that may be relevant for resistance. These include rapid triacylglycerols degradation, galactolipid peroxidation, constitutive higher SA level and expression of the defensin VviAMP1.
Related
- Whole plant metabolomic analysis reveals contrasted responses in tolerant and susceptible grapevine cultivars to infection with different isolates of Neofusicoccum parvum, a major trunk disease fungus
- Histopathology of Rhizoctonia root and crown rot of sugar beet reveals differential responses in susceptible and resistant cultivars to invasion by Rhizoctonia solani AG 2-2
- From Willingness to Adoption: Information, Perception, and the Diffusion of Fungus-Resistant Grapevine Varieties in Southern France
- MtbAIM: AI/ML-guided endpoint-specific prediction of small-molecule activity across drug-susceptible and drug-resistant Mycobacterium tuberculosis
- Vector competence for dengue-2 virus of permethrin resistant/ susceptible Aedes aegypti sub-populations from selected sites in Lamu County, Kenya
BibTeX
@article{guche2026distinct,
title = {{Distinct lipid and hormonal signatures reveal divergent responses to Erysiphe necator in resistant and susceptible grapevine genotypes.}},
author = {Mikias D Guche and Lorenza Dalla Costa and Alessandra Lanubile and Axel Mithöfer and Domenico Masuero and Francesco Trenti and Mar Garcia-Aloy and Silvia Vezzulli and Urska Vrhovsek and Graziano Guella and Mickael Malnoy and Adriano Marocco and Stefania Pilati},
journal = {BMC plant biology},
year = {2026},
month = aug,
doi = {10.1186/s12870-026-09654-9},
url = {https://europepmc.org/article/MED/42958022},
note = {CC BY},
}
RIS
TY - JOUR
TI - Distinct lipid and hormonal signatures reveal divergent responses to Erysiphe necator in resistant and susceptible grapevine genotypes.
AU - Mikias D Guche
AU - Lorenza Dalla Costa
AU - Alessandra Lanubile
AU - Axel Mithöfer
AU - Domenico Masuero
AU - Francesco Trenti
AU - Mar Garcia-Aloy
AU - Silvia Vezzulli
AU - Urska Vrhovsek
AU - Graziano Guella
AU - Mickael Malnoy
AU - Adriano Marocco
AU - Stefania Pilati
PY - 2026
DA - 2026/08/01
JO - BMC plant biology
DO - 10.1186/s12870-026-09654-9
UR - https://europepmc.org/article/MED/42958022
AB - Background Powdery mildew, caused by the obligate biotroph Erysiphe necator, represents a major threat to grapevine production worldwide. Host-mediated resistance offers a sustainable alternative to chemical fungicides. To elucidate the role of plant membrane lipid modifications and oxylipin accumulation in pathogen perception and response, a controlled infection experiment was conducted comparing a resistant hybrid (NY_39) from the Edmund Mach Foundation germplasm collection with a susceptible variety (cv. 'Teroldego').Results A lipidomic approach was integrated with hormone profiling and lipoxygenase (LOX) gene expression analysis. Significant lipid modulation was observed following E. necator inoculation, highlighting the plasticity of membrane and storage lipids metabolism. A rapid decrease in triacylglycerol content was measured in NY_39 at 12 h post-inoculation (hpi), whereas the opposite trend occurred in 'Teroldego', suggesting divergent metabolic rearrangements. A fast galactolipids peroxidation occurred in NY_39, while a delayed accumulation of phosphatidic acid was observed in 'Teroldego' at 48 hpi. The resistant genotype exhibited higher constitutive levels of salicylic acid as well as the expression of the VviAMP1 defensin, a small cysteine-rich protein with broad-spectrum antifungal activity. VviPR10-s11 and VviWRKY51, related to the synthesis of lignin and stilbenoid phytoalexins, were induced at 12 hpi in both genotypes. Within the LOX family, significant up-regulation of the 9-LOX VviLOX1a and the 13-LOXs VviLOX9 and VviLOXO was specific to 'Teroldego' at 12 hpi, despite a higher constitutive level of VviLOX9 in NY_39.Conclusions This work identifies key metabolic, signalling and gene expression features in the selected hybrid that may be relevant for resistance. These include rapid triacylglycerols degradation, galactolipid peroxidation, constitutive higher SA level and expression of the defensin VviAMP1.
SN - 1471-2229
ER -