Synthesis, Characterization and In Vitro Cytotoxic Assay of La(III) Complex with Chlorambucil
- 1Chemistry Institute, Federal University of Mato Grosso do Sul, Campo Grande 79079-900, MS, Brazil
- 2Faculty of Pharmacy, Food and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande 79079-900, MS, Brazil
Abstract
This work studies the synthesis of complexes using chlorambucil (La-CLA) as a ligand and lanthanum as a metallic center, in order to enhance their biological activities. The compounds produced were characterized by infrared spectroscopy and 13C and 1H nuclear magnetic resonance; the results obtained indicate that coordination bonds formed between the ligands and that La3+ was present in a bidentate way through carboxylic groups in the complexes. Elemental and thermogravimetric analyses indicate that the stoichiometry obtained for chlorambucil complexes was a 2:1 ligand:metal ratio. The anticancer activity assays indicated that the La-CLA complex showed better activity than the ligands against the cell lines K562 (Chronic Myeloid Leukemia), HT-29 (Colorectal Cancer), Caco 2 (Human Colorectal Adenocarcinoma) and B16-F10 (Murine Melanoma), with GI50 values in the range of 0.31–31.86 μM. The La-CLA complex showed a selectivity index (SI) equal to 15.64 for the K562 cell line with respect to normal J774 cells (murine macrophages).
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BibTeX
@article{timoteo2026synthesis,
title = {{Synthesis, Characterization and In Vitro Cytotoxic Assay of La(III) Complex with Chlorambucil}},
author = {Fernanda Timoteo and Kristiane Fanti Del Pino and Danielle Bogo and Vasilii Khripun and Franciele Garcia Baveloni and Sabrina Epifânio Soares de Souza and Brunna Mercedes Barboza Borges and Guilherme Pereira Biazoto and Diego Boldo and Adriana Pereira Duarte and Adriana da Silva Posso and Marco Antonio Utrera Martines},
journal = {Molecules},
year = {2026},
month = oct,
doi = {10.3390/molecules31203584},
url = {https://www.mdpi.com/1420-3049/31/20/3584},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Synthesis, Characterization and In Vitro Cytotoxic Assay of La(III) Complex with Chlorambucil
AU - Fernanda Timoteo
AU - Kristiane Fanti Del Pino
AU - Danielle Bogo
AU - Vasilii Khripun
AU - Franciele Garcia Baveloni
AU - Sabrina Epifânio Soares de Souza
AU - Brunna Mercedes Barboza Borges
AU - Guilherme Pereira Biazoto
AU - Diego Boldo
AU - Adriana Pereira Duarte
AU - Adriana da Silva Posso
AU - Marco Antonio Utrera Martines
PY - 2026
DA - 2026/10/01
JO - Molecules
DO - 10.3390/molecules31203584
UR - https://www.mdpi.com/1420-3049/31/20/3584
AB - This work studies the synthesis of complexes using chlorambucil (La-CLA) as a ligand and lanthanum as a metallic center, in order to enhance their biological activities. The compounds produced were characterized by infrared spectroscopy and 13C and 1H nuclear magnetic resonance; the results obtained indicate that coordination bonds formed between the ligands and that La3+ was present in a bidentate way through carboxylic groups in the complexes. Elemental and thermogravimetric analyses indicate that the stoichiometry obtained for chlorambucil complexes was a 2:1 ligand:metal ratio. The anticancer activity assays indicated that the La-CLA complex showed better activity than the ligands against the cell lines K562 (Chronic Myeloid Leukemia), HT-29 (Colorectal Cancer), Caco 2 (Human Colorectal Adenocarcinoma) and B16-F10 (Murine Melanoma), with GI50 values in the range of 0.31–31.86 μM. The La-CLA complex showed a selectivity index (SI) equal to 15.64 for the K562 cell line with respect to normal J774 cells (murine macrophages).
SN - 1420-3049
ER -