Differences in RNA Binding Between Segmented and Non-Segmented Negative-Strand Virus Nucleocapsids
Abstract
Segmented and non-segmented negative-strand RNA viruses share the same general pathway for genome transcription, which generates messenger RNA, and genome replication which duplicates the viral RNA. These processes are performed by the viral polymerase and necessitate the viral RNA to be coated by a non-covalent polymer of nucleoproteins known as nucleocapsid. The non-segmented negative-strand RNA viruses (nsNSVs) have rigid nucleocapsids covering the entire tightly bound genome and require a phosphoprotein cofactor for proper replication and transcription by the polymerase, while the segmented negative-strand RNA viruses (sNSVs) have very flexible nucleocapsids with only few nucleotides tightly bound to each nucleoprotein, and their viral RNA genome ends are directly bound to the polymerase. We discuss here how the differences in RNA binding are likely to be crucial for proper replication and transcription in both nsNSVs and sNSVs.
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BibTeX
@article{ruigrok2026differences,
title = {{Differences in RNA Binding Between Segmented and Non-Segmented Negative-Strand Virus Nucleocapsids}},
author = {Rob Ruigrok and Allison Ballandras-Colas and Thibaut Crépin and Hélène Malet and Dan Kolakofsky},
journal = {Microorganisms},
year = {2026},
month = may,
doi = {10.3390/microorganisms14061194},
url = {https://cnrs.hal.science/hal-05808165v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Differences in RNA Binding Between Segmented and Non-Segmented Negative-Strand Virus Nucleocapsids
AU - Rob Ruigrok
AU - Allison Ballandras-Colas
AU - Thibaut Crépin
AU - Hélène Malet
AU - Dan Kolakofsky
PY - 2026
DA - 2026/05/26
JO - Microorganisms
DO - 10.3390/microorganisms14061194
UR - https://cnrs.hal.science/hal-05808165v1
AB - Segmented and non-segmented negative-strand RNA viruses share the same general pathway for genome transcription, which generates messenger RNA, and genome replication which duplicates the viral RNA. These processes are performed by the viral polymerase and necessitate the viral RNA to be coated by a non-covalent polymer of nucleoproteins known as nucleocapsid. The non-segmented negative-strand RNA viruses (nsNSVs) have rigid nucleocapsids covering the entire tightly bound genome and require a phosphoprotein cofactor for proper replication and transcription by the polymerase, while the segmented negative-strand RNA viruses (sNSVs) have very flexible nucleocapsids with only few nucleotides tightly bound to each nucleoprotein, and their viral RNA genome ends are directly bound to the polymerase. We discuss here how the differences in RNA binding are likely to be crucial for proper replication and transcription in both nsNSVs and sNSVs.
SN - 2076-2607
ER -