Early injury responses to nutrient treatment in adult Drosophila limb
- 1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA
- 2Department of Computing and Mathematical Sciences, California Institute of Technology, Pasadena, CA 91125, USA
Abstract
It was previously observed that partial regrowth can be induced to begin in adult Drosophila limb by modulating nutrients. In this work, to further investigate this observation, we characterized the early cellular- and tissue-level processes that are promoted by nutrient treatment after injury. We find that response to nutrient treatment can be readily identified within hours, by alteration in wound healing. Subsequently, at day scale, dramatic reversal of large-scale tissue loss occurs in treated flies. Finally, at week scale, tissue remodeling begins, and a dome-shaped protrusion forms at the injury site. Using single-nucleus RNA sequencing at this stage, we find widespread transcriptional modulations across cell types. Distinct muscle and epithelial cell types emerge that are enriched in mitotic and morphogenetic genes, including those associated with regeneration in larval tissues. These tissue-, cell-, and gene-level processes characterized here provide a framework for the previously observed partial limb growth and for further investigating the suggestion of regeneration induction in adult Drosophila through nutrient treatment.
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BibTeX
@article{li2026early,
title = {{Early injury responses to nutrient treatment in adult Drosophila limb}},
author = {Yutian Li and Jesus del Rio Salgado and Gloria Bates and Zevin J. Condiotte and Maya Caskey and Christian M. Dimayuga and Brian Williams and Ingileif Hallgrimsdottir and Lior Pachter and Lea Goentoro},
journal = {Biology Open},
year = {2026},
month = oct,
doi = {10.1242/bio.062737},
url = {https://doi.org/10.1242/bio.062737},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Early injury responses to nutrient treatment in adult Drosophila limb
AU - Yutian Li
AU - Jesus del Rio Salgado
AU - Gloria Bates
AU - Zevin J. Condiotte
AU - Maya Caskey
AU - Christian M. Dimayuga
AU - Brian Williams
AU - Ingileif Hallgrimsdottir
AU - Lior Pachter
AU - Lea Goentoro
PY - 2026
DA - 2026/10/23
JO - Biology Open
DO - 10.1242/bio.062737
UR - https://doi.org/10.1242/bio.062737
AB - It was previously observed that partial regrowth can be induced to begin in adult Drosophila limb by modulating nutrients. In this work, to further investigate this observation, we characterized the early cellular- and tissue-level processes that are promoted by nutrient treatment after injury. We find that response to nutrient treatment can be readily identified within hours, by alteration in wound healing. Subsequently, at day scale, dramatic reversal of large-scale tissue loss occurs in treated flies. Finally, at week scale, tissue remodeling begins, and a dome-shaped protrusion forms at the injury site. Using single-nucleus RNA sequencing at this stage, we find widespread transcriptional modulations across cell types. Distinct muscle and epithelial cell types emerge that are enriched in mitotic and morphogenetic genes, including those associated with regeneration in larval tissues. These tissue-, cell-, and gene-level processes characterized here provide a framework for the previously observed partial limb growth and for further investigating the suggestion of regeneration induction in adult Drosophila through nutrient treatment.
SN - 2046-6390
ER -