Skip to content
DraconiticResearch
Search Sources API
203,234 studies31 sourcesUpdated 36 min ago
Peer-reviewedCC BY 4.0Acta crystallographica Section F : Structural biology communications [2014-...]

Serendipitous crystal structure of mJuniper, a rationally designed cyan fluorescent protein

  • Alpay Aydin
  • Nathan Fraikin
  • Priscillia Lagoutte
  • Christian Lesterlin
  • Laurent Terradot
Open at sourcePDFDOICite

Abstract

mJuniper is a recently developed monomeric cyan fluorescent protein (CFP) optimized for bacterial imaging, distinguished by the fastest maturation kinetics recorded for a CFP. mJuniper shows significant promise for time-lapse microscopy, but was used here to improve the solubility of a target protein. Here, we report the high-resolution X-ray structure of mJuniper, obtained serendipitously during an attempted structural characterization of CagG, an essential component of the Helicobacter pylori Cag type IV secretion system. The structure reveals a classical β-barrel scaffold, as well as a chromophore with two rotameric states, resulting from specific radiation damage occurring in its vicinity.

Related

  1. Rationally Designed Phenyl- and Phenanthryl-Based Imidazole Hybrids for the Treatment of Breast Cancer—A Structure–Activity Relationship Study
    Bharathi Hassan Ganesh, Preethi G. Anantharaju, Amritha Sukumaran +5
    MoleculesPeer-reviewedCC BY 4.0Full textOrganic chemistryCrossrefEurope PMC
  2. Synthesis and crystal structure of a one-dimensional Hg(II) coordination polymer with two distinct metal centers and its selective fluorescent sensing of Ni²⁺
    Zohreh Derikvand, Ameneh Jahani Chegeni
    Scientific ReportsPeer-reviewedCC BY-NC-ND 4.0Full textInorganic & organometallic chemistryCrossref
  3. Structure–Performance Relationships in Newly Designed Linear Polyamines for Advanced CO2 Sorption
    Elise Lengaigne, Marie Kobylarski, Nicolas Pinochet
    ChemRxivPreprintCC BYFull textGeneral chemistryChemRxiv
  4. MicroED Guided Structure Property Relationships in Mechanochemically Synthesized Fluorescent Cocrystals
    Nabadeep Kalita, Takanori Nakane, Akihiro Kawamoto +5
    ChemRxivPreprintCC BYFull textPhysical chemistryChemRxiv
  5. The X‐ray crystallographic structure of a designed, self‐assembling, and heme‐binding protein
    Iago de Assis Modenez, Véronique Roig‐Zamboni, Philippe Leone +2
    Protein SciencePeer-reviewedCC BY 4.0Full textBiophysicsGeneral biologyHALPMC

Cite

BibTeXRIS
BibTeX
@article{aydin2026serendipitous,
  title = {{Serendipitous crystal structure of mJuniper, a rationally designed cyan fluorescent protein}},
  author = {Alpay Aydin and Nathan Fraikin and Priscillia Lagoutte and Christian Lesterlin and Laurent Terradot},
  journal = {Acta crystallographica Section F : Structural biology communications [2014-...]},
  year = {2026},
  month = sep,
  doi = {10.1107/s2053230x26009052},
  url = {https://hal.science/hal-05767043v1},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Serendipitous crystal structure of mJuniper, a rationally designed cyan fluorescent protein
AU  - Alpay Aydin
AU  - Nathan Fraikin
AU  - Priscillia Lagoutte
AU  - Christian Lesterlin
AU  - Laurent Terradot
PY  - 2026
DA  - 2026/09/17
JO  - Acta crystallographica Section F : Structural biology communications [2014-...]
DO  - 10.1107/s2053230x26009052
UR  - https://hal.science/hal-05767043v1
AB  - mJuniper is a recently developed monomeric cyan fluorescent protein (CFP) optimized for bacterial imaging, distinguished by the fastest maturation kinetics recorded for a CFP. mJuniper shows significant promise for time-lapse microscopy, but was used here to improve the solubility of a target protein. Here, we report the high-resolution X-ray structure of mJuniper, obtained serendipitously during an attempted structural characterization of CagG, an essential component of the Helicobacter pylori Cag type IV secretion system. The structure reveals a classical β-barrel scaffold, as well as a chromophore with two rotameric states, resulting from specific radiation damage occurring in its vicinity.
SN  - 2053-230X
ER  -