Multimodal Hyperspectral Imaging Unveils Pigment Rearrangement in the Diatom Cyclotella meneghiniana under Changing Physicochemical Conditions.
6 affiliations
- 1Institute of Chemical Physics, Faculty of Physics, Vilnius University, Sauletekio Av. 9, III bld., 10222Vilnius, Lithuania.
- 2Department of Molecular Compound Physics, State Research Institute Center for Physical Sciences and Technology, Sauletekio Av. 3, 10257Vilnius, Lithuania.
- 3Neuroscience Research Center, College of Medicine, Jiaxing University, Jiaxing, Zhejiang314001, China.
- 4Institute of Photonics and Nanotechnology, Faculty of Physics, Vilnius University, Sauletekio Av. 3, 10257Vilnius, Lithuania.
- 5Department of Optoelectronics, State Research Institute Center for Physical Sciences and Technology, Sauletekio Av. 3, 10257Vilnius, Lithuania.
- 6Institute of Molecular Biosciences, Department of Biosciences, Goethe University Frankfurt, Max-von-Laue-Str. 9, 60438Frankfurt, Germany.
Abstract
Revealing the spatial organization and stress-induced rearrangements of the photosynthetic machinery in thylakoids of diatoms is crucial for understanding their metabolic responses and/or adaptation. Using a multimodal framework that combines coherent anti-Stokes Raman scattering (CARS), two-photon excited fluorescence, and confocal fluorescence microscopy with machine learning, we investigated the spatial pigment distribution in thylakoids of Cyclotella meneghiniana. Under physiological conditions, we observed dense central packing of fucoxanthins (Fx's). In contrast, the peripheral girdle lamella had a lower packing of mostly red-absorbing Fx accompanied by enhanced 710-750 nm chlorophyll (Chl) emission. Glycerol treatment induced thylakoid disintegration, which was reflected in decreased resonance-to-nonresonance CARS ratios (Fx disordering), emergence of a 690-700 nm fluorescence component, and 680 nm band narrowing (possibly fucoxanthin-chlorophyll protein (FCP) aggregation), as well as 640 nm emission (Chl c release). Our findings show that this multimodal framework provides a powerful, label-free tool for mapping the photosynthetic machinery and subcellular metabolic responses.
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BibTeX
@article{golubewa2026multimodal,
title = {{Multimodal Hyperspectral Imaging Unveils Pigment Rearrangement in the Diatom Cyclotella meneghiniana under Changing Physicochemical Conditions.}},
author = {Lena Golubewa and Yaraslau Padrez and Patricija Zemaityte and Paulius Rindzevicius and Andrej Dementjev and Kipras Mažeika and Augustas Vaitkevičius and Claudia Büchel and Andrius Gelzinis},
journal = {The journal of physical chemistry letters},
year = {2026},
month = oct,
doi = {10.1021/acs.jpclett.6c02711},
url = {https://europepmc.org/article/MED/42918216},
note = {CC BY},
}
RIS
TY - JOUR
TI - Multimodal Hyperspectral Imaging Unveils Pigment Rearrangement in the Diatom Cyclotella meneghiniana under Changing Physicochemical Conditions.
AU - Lena Golubewa
AU - Yaraslau Padrez
AU - Patricija Zemaityte
AU - Paulius Rindzevicius
AU - Andrej Dementjev
AU - Kipras Mažeika
AU - Augustas Vaitkevičius
AU - Claudia Büchel
AU - Andrius Gelzinis
PY - 2026
DA - 2026/10/01
JO - The journal of physical chemistry letters
DO - 10.1021/acs.jpclett.6c02711
UR - https://europepmc.org/article/MED/42918216
AB - Revealing the spatial organization and stress-induced rearrangements of the photosynthetic machinery in thylakoids of diatoms is crucial for understanding their metabolic responses and/or adaptation. Using a multimodal framework that combines coherent anti-Stokes Raman scattering (CARS), two-photon excited fluorescence, and confocal fluorescence microscopy with machine learning, we investigated the spatial pigment distribution in thylakoids of Cyclotella meneghiniana. Under physiological conditions, we observed dense central packing of fucoxanthins (Fx's). In contrast, the peripheral girdle lamella had a lower packing of mostly red-absorbing Fx accompanied by enhanced 710-750 nm chlorophyll (Chl) emission. Glycerol treatment induced thylakoid disintegration, which was reflected in decreased resonance-to-nonresonance CARS ratios (Fx disordering), emergence of a 690-700 nm fluorescence component, and 680 nm band narrowing (possibly fucoxanthin-chlorophyll protein (FCP) aggregation), as well as 640 nm emission (Chl c release). Our findings show that this multimodal framework provides a powerful, label-free tool for mapping the photosynthetic machinery and subcellular metabolic responses.
SN - 1948-7185
ER -