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Peer-reviewedCC BY 4.0International Journal of Molecular Sciences

A Time-Course Transcriptomic Resource for Early Embryogenesis in Lethenteron reissneri

  • Weiyin Xu1,2
  • Jun Li1,2
  • Guiyao Wang1,2
  • Qingkui Bai1,2
  • Huiyi Fu1,2
  • Ji Zhao1,2
  • Shuiqing Yu1
  • Ting Zhu1,2
  1. 1College of Life Sciences, Liaoning Normal University, Dalian 116081, China
  2. 2Lamprey Research Center, Liaoning Normal University, Dalian 116081, China
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Abstract

Continuous developmental transcriptomic data remain limited for jawless vertebrates. We generated an RNA-seq time series spanning 2 h post-fertilization (hpf; fertilization stage) to 10 days post-fertilization (dpf; hatching stage) in Lethenteron reissneri, with 14 developmental stages and three biological replicates per stage. We examined global expression patterns, differential expression between adjacent stages, functional enrichment, and stage-level co-expression, and assessed representative expression trends using reverse transcription quantitative PCR (RT-qPCR). Gene-expression profiles changed progressively across development. One notable interval was 2.5–3 dpf (late blastula to early gastrula), when upregulated genes were broadly enriched for germ-layer formation, axial patterning, neural development, and morphogenesis rather than the RNA-processing, ribosome-biogenesis, and translation terms prominent at earlier stages. Stage-level co-expression analysis identified a module enriched for development-related terms, and differential expression, functional annotation, and temporal profiles were used to compile a list of 154 candidate developmental genes. This study provides a continuous transcriptomic resource for early L. reissneri embryogenesis and a temporal framework for subsequent studies of lamprey development.

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Cite

BibTeXRIS
BibTeX
@article{xu2026timecourse,
  title = {{A Time-Course Transcriptomic Resource for Early Embryogenesis in Lethenteron reissneri}},
  author = {Weiyin Xu and Jun Li and Guiyao Wang and Qingkui Bai and Huiyi Fu and Ji Zhao and Shuiqing Yu and Ting Zhu},
  journal = {International Journal of Molecular Sciences},
  year = {2026},
  month = oct,
  doi = {10.3390/ijms27209001},
  url = {https://www.mdpi.com/1422-0067/27/20/9001},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - A Time-Course Transcriptomic Resource for Early Embryogenesis in Lethenteron reissneri
AU  - Weiyin Xu
AU  - Jun Li
AU  - Guiyao Wang
AU  - Qingkui Bai
AU  - Huiyi Fu
AU  - Ji Zhao
AU  - Shuiqing Yu
AU  - Ting Zhu
PY  - 2026
DA  - 2026/10/01
JO  - International Journal of Molecular Sciences
DO  - 10.3390/ijms27209001
UR  - https://www.mdpi.com/1422-0067/27/20/9001
AB  - Continuous developmental transcriptomic data remain limited for jawless vertebrates. We generated an RNA-seq time series spanning 2 h post-fertilization (hpf; fertilization stage) to 10 days post-fertilization (dpf; hatching stage) in Lethenteron reissneri, with 14 developmental stages and three biological replicates per stage. We examined global expression patterns, differential expression between adjacent stages, functional enrichment, and stage-level co-expression, and assessed representative expression trends using reverse transcription quantitative PCR (RT-qPCR). Gene-expression profiles changed progressively across development. One notable interval was 2.5–3 dpf (late blastula to early gastrula), when upregulated genes were broadly enriched for germ-layer formation, axial patterning, neural development, and morphogenesis rather than the RNA-processing, ribosome-biogenesis, and translation terms prominent at earlier stages. Stage-level co-expression analysis identified a module enriched for development-related terms, and differential expression, functional annotation, and temporal profiles were used to compile a list of 154 candidate developmental genes. This study provides a continuous transcriptomic resource for early L. reissneri embryogenesis and a temporal framework for subsequent studies of lamprey development.
SN  - 1422-0067
ER  -