3D MERFISH reveals cell-type diversity generated by mRNA partitioning in spiralian embryogenesis
- 1Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA, USA
Abstract
Cell type diversification during development is often attributed to differential gene transcription. Here, we show that, during Crepidula atrasolea embryogenesis, transcriptomically distinct cell types arise through differential distribution of pre-existing mRNAs. Combining immunofluorescence, live imaging, and whole-mount 3D spatial transcriptomics (MERFISH) of over 40 embryos, we show that many mRNAs form subcellular aggregates during mitosis, which segregate asymmetrically between daughter cells through microtubule associations. Cell divisions thus simultaneously increase cell number and diversity, producing six transcriptomically distinct cell quartets in the 24-cell stage embryo. We modeled aggregate compositions across cell divisions using transcript abundance and quantified transcript-specific affinity. These results establish mRNA sorting and inheritance as a scalable cell type patterning mechanism and provide a framework for investigating how mRNA segregation shapes animal development.
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BibTeX
@misc{wang2026merfish,
title = {{3D MERFISH reveals cell-type diversity generated by mRNA partitioning in spiralian embryogenesis}},
author = {Y. Wang and T. Patil and S. Neal and I. K. S. Jenie and E. Ignatoff and B. Bintu and D. Lyons},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.05.756875},
url = {https://www.biorxiv.org/content/10.64898/2026.10.05.756875v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - 3D MERFISH reveals cell-type diversity generated by mRNA partitioning in spiralian embryogenesis
AU - Y. Wang
AU - T. Patil
AU - S. Neal
AU - I. K. S. Jenie
AU - E. Ignatoff
AU - B. Bintu
AU - D. Lyons
PY - 2026
DA - 2026/10/05
PB - bioRxiv
DO - 10.64898/2026.10.05.756875
UR - https://www.biorxiv.org/content/10.64898/2026.10.05.756875v1
AB - Cell type diversification during development is often attributed to differential gene transcription. Here, we show that, during Crepidula atrasolea embryogenesis, transcriptomically distinct cell types arise through differential distribution of pre-existing mRNAs. Combining immunofluorescence, live imaging, and whole-mount 3D spatial transcriptomics (MERFISH) of over 40 embryos, we show that many mRNAs form subcellular aggregates during mitosis, which segregate asymmetrically between daughter cells through microtubule associations. Cell divisions thus simultaneously increase cell number and diversity, producing six transcriptomically distinct cell quartets in the 24-cell stage embryo. We modeled aggregate compositions across cell divisions using transcript abundance and quantified transcript-specific affinity. These results establish mRNA sorting and inheritance as a scalable cell type patterning mechanism and provide a framework for investigating how mRNA segregation shapes animal development.
ER -