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Peer-reviewedCC BY 4.0Frontiers in Plant Science

A rapid and simple vacuum infiltration system of germinated seeds for plant genetic transformation

  • Heru Yang1
  • Jiaxue Yu1
  • Weiyan Wang1
  • Ye Chai1
  • Taicheng Jin1,2
  • Liping Yang1,2
  1. 1The School of Life Sciences, Jilin Normal University
  2. 2The Key Laboratory of Jilin Province for Plant Resources Science and Green Production
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Abstract

Plant transformation methods play a crucial role in molecular breeding that enhances crop yield and quality. The genetic transformation of soybean is mainly achieved through Agrobacterium -mediated methods. To solve the problem of long cycles in plant transformation, this study established and optimized a rapid and simple transformation system, namely the vacuum infiltration system of germinated seeds, in soybean ( Glycine max (Linn.) Merr.). The expression of β-glucuronidase (GUS) in soybean was achieved using this system, which did not need sterile conditions. The results of reverse transcription quantitative PCR (RT-qPCR) analysis indicated that the GUS gene was stably expressed in T1-generation transgenic plants. Compared with traditional transformation methods, this system is rapid and simple and does not require tissue culture procedures. Moreover, to explore the CRISPR/Cas9 gene editing system in combination with vacuum infiltration of germinated seeds, we used the gene editing vector pHEE- pds to knock out the PDS gene in tobacco ( Nicotiana tabacum L.). In addition, the visual RUBY reporter gene combined with the vacuum infiltration of the cotyledons was applied to the soybean transformation, which effectively improves the screening of candidate transformants.

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Cite

BibTeXRIS
BibTeX
@article{yang2026rapid,
  title = {{A rapid and simple vacuum infiltration system of germinated seeds for plant genetic transformation}},
  author = {Heru Yang and Jiaxue Yu and Weiyan Wang and Ye Chai and Taicheng Jin and Liping Yang},
  journal = {Frontiers in Plant Science},
  year = {2026},
  month = oct,
  doi = {10.3389/fpls.2026.1993488},
  url = {https://www.frontiersin.org/articles/10.3389/fpls.2026.1993488/full},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - A rapid and simple vacuum infiltration system of germinated seeds for plant genetic transformation
AU  - Heru Yang
AU  - Jiaxue Yu
AU  - Weiyan Wang
AU  - Ye Chai
AU  - Taicheng Jin
AU  - Liping Yang
PY  - 2026
DA  - 2026/10/26
JO  - Frontiers in Plant Science
DO  - 10.3389/fpls.2026.1993488
UR  - https://www.frontiersin.org/articles/10.3389/fpls.2026.1993488/full
AB  - Plant transformation methods play a crucial role in molecular breeding that enhances crop yield and quality. The genetic transformation of soybean is mainly achieved through Agrobacterium -mediated methods. To solve the problem of long cycles in plant transformation, this study established and optimized a rapid and simple transformation system, namely the vacuum infiltration system of germinated seeds, in soybean ( Glycine max (Linn.) Merr.). The expression of β-glucuronidase (GUS) in soybean was achieved using this system, which did not need sterile conditions. The results of reverse transcription quantitative PCR (RT-qPCR) analysis indicated that the GUS gene was stably expressed in T1-generation transgenic plants. Compared with traditional transformation methods, this system is rapid and simple and does not require tissue culture procedures. Moreover, to explore the CRISPR/Cas9 gene editing system in combination with vacuum infiltration of germinated seeds, we used the gene editing vector pHEE- pds to knock out the PDS gene in tobacco ( Nicotiana tabacum L.). In addition, the visual RUBY reporter gene combined with the vacuum infiltration of the cotyledons was applied to the soybean transformation, which effectively improves the screening of candidate transformants.
SN  - 1664-462X
ER  -