L(-)-Borneol-Containing Nanoparticles Ameliorate Oxidative Stress and Inflammatory Responses In Vitro and in a Mouse Model of COPD
4 affiliations
- 1Pharmacy College, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China
- 2College of Food and Health, Zhejiang A & F University, Hangzhou 311300, China
- 3Blumea Balsamifera Research Center, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China
- 4Guizhou Research Center of Conservation and Utilization of Wild Resources of Karst Traditional Chinese Medicine and Ethnic Medicine, Anshun 561000, China
Abstract
L(−)-borneol (LB), a natural monoterpene, possesses anti-inflammatory and antioxidant properties but suffers from poor aqueous solubility, high volatility, and low oral bioavailability. Here, an oral nanoparticle delivery system was developed via horseradish peroxidase-mediated oxidative crosslinking of polyethylene glycol, generating L(−)-borneol -containing PEG nanoparticles (LB NPs). The optimized LB NPs exhibited uniform spherical morphology with an average size of 70.4 ± 4.7 nm, a negative zeta potential of −19.3 ± 0.5 mV, high encapsulation efficiency (95.09 ± 0.44%), and drug loading of 6.47 ± 0.78%. In lipopolysaccharide (LPS)-stimulated macrophages, LB NPs reduced intracellular reactive oxygen species (ROS), malondialdehyde (MDA), and myeloperoxidase (MPO) levels while restoring glutathione (GSH), indicating enhanced antioxidant protection. Following oral administration, LB NPs produced detectable pulmonary-associated fluorescence. In a cigarette smoke/LPS-induced COPD mouse model, oral LB NPs attenuated pulmonary inflammation, collagen deposition, and histopathological injury. Improvements in hepatic and renal biochemical and histopathological abnormalities were also observed. A 21-day repeated oral administration study showed no apparent treatment-related systemic toxicity. These findings suggest that LB-containing PEG nanoparticles improve the pharmaceutical properties and biological activity of LB, supporting further investigation as a potential adjunctive strategy for COPD.
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BibTeX
@article{yang2026lborneolcontaining,
title = {{L(-)-Borneol-Containing Nanoparticles Ameliorate Oxidative Stress and Inflammatory Responses In Vitro and in a Mouse Model of COPD}},
author = {Lixia Yang and Gang Liu and Qiong Shen and Jingjing Huang and Xiong Han and Zhanchi Xu and Yuxin Pang and Yanfei Li and Yuchen Liu},
journal = {Biomolecules},
year = {2026},
month = nov,
doi = {10.3390/biom16111622},
url = {https://www.mdpi.com/2218-273X/16/11/1622},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - L(-)-Borneol-Containing Nanoparticles Ameliorate Oxidative Stress and Inflammatory Responses In Vitro and in a Mouse Model of COPD
AU - Lixia Yang
AU - Gang Liu
AU - Qiong Shen
AU - Jingjing Huang
AU - Xiong Han
AU - Zhanchi Xu
AU - Yuxin Pang
AU - Yanfei Li
AU - Yuchen Liu
PY - 2026
DA - 2026/11/07
JO - Biomolecules
DO - 10.3390/biom16111622
UR - https://www.mdpi.com/2218-273X/16/11/1622
AB - L(−)-borneol (LB), a natural monoterpene, possesses anti-inflammatory and antioxidant properties but suffers from poor aqueous solubility, high volatility, and low oral bioavailability. Here, an oral nanoparticle delivery system was developed via horseradish peroxidase-mediated oxidative crosslinking of polyethylene glycol, generating L(−)-borneol -containing PEG nanoparticles (LB NPs). The optimized LB NPs exhibited uniform spherical morphology with an average size of 70.4 ± 4.7 nm, a negative zeta potential of −19.3 ± 0.5 mV, high encapsulation efficiency (95.09 ± 0.44%), and drug loading of 6.47 ± 0.78%. In lipopolysaccharide (LPS)-stimulated macrophages, LB NPs reduced intracellular reactive oxygen species (ROS), malondialdehyde (MDA), and myeloperoxidase (MPO) levels while restoring glutathione (GSH), indicating enhanced antioxidant protection. Following oral administration, LB NPs produced detectable pulmonary-associated fluorescence. In a cigarette smoke/LPS-induced COPD mouse model, oral LB NPs attenuated pulmonary inflammation, collagen deposition, and histopathological injury. Improvements in hepatic and renal biochemical and histopathological abnormalities were also observed. A 21-day repeated oral administration study showed no apparent treatment-related systemic toxicity. These findings suggest that LB-containing PEG nanoparticles improve the pharmaceutical properties and biological activity of LB, supporting further investigation as a potential adjunctive strategy for COPD.
SN - 2218-273X
ER -