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Peer-reviewedCC BY 4.0International Journal of Molecular Sciences

Effects of a Two-Strain Lactobacillus Formulation (Lactobacillus rhamnosus L34 and Lactobacillus paracasei B13) on Inflammatory and Oxidative Markers, Epithelial Junction Proteins, and Fecal Microbiota in Rats with DNBS-Induced Colitis

  • Pakkapon Rattanachaisit1,2
  • Nathawadee Lerttanatum3
  • Somying Tumwasorn4
  • Natthaya Chuaypen5,6
  • Thasinas Dissayabutra5,6
  • Duangporn Werawatganon1,2
6 affiliations
  1. 1Center of Excellence in Alternative and Complementary Medicine for Gastrointestinal and Liver Diseases, Chulalongkorn University, Bangkok 10330, Thailand
  2. 2Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
  3. 3Department of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
  4. 4Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
  5. 5Center of Excellence in Hepatitis and Liver Cancer, Chulalongkorn University, Bangkok 10330, Thailand
  6. 6Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
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Abstract

Inflammatory bowel disease (IBD) involves dysregulated mucosal immunity, oxidative stress, impaired epithelial barrier integrity and gut microbial dysbiosis. We evaluated a two-strain formulation of Lactobacillus rhamnosus L34 and Lactobacillus paracasei B13 in DNBS-induced colitis. Twenty-four male Sprague Dawley rats were randomised into three groups (n = 8): control, acute colitis (AC; DNBS 35 mg/0.25 mL per rat rectally on days 0 and 1), and probiotic treated (RP; 3.6 × 109 CFU daily by gavage, days −3 to 6). DNBS raised Ameho histological scores, serum TNF-α, colonic MDA and MPO positivity and lowered IL-10, occludin and claudin-1 (all p < 0.05). Relative to AC, probiotic-treated rats showed lower TNF-α (p = 0.002) and MPO positivity (p < 0.001) and higher IL-10 (p = 0.001), occludin (p = 0.014) and claudin-1 (p = 0.001). Colonic MDA and Ameho scores were numerically lower in the RP group but these differences did not reach statistical significance (p = 0.085 and p = 0.310). Serum IL-17A was unchanged. Microbial community composition differed among groups (PERMANOVA pseudo-F = 5.798, R2 = 0.356, p = 0.0001), with the probiotic-associated community distinct from both control and colitis communities; distance-based analysis detected no convergence toward the control community. The formulation attenuated inflammatory and barrier outcomes and altered microbiota composition without restoring it.

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Cite

BibTeXRIS
BibTeX
@article{rattanachaisit2026effects,
  title = {{Effects of a Two-Strain Lactobacillus Formulation (Lactobacillus rhamnosus L34 and Lactobacillus paracasei B13) on Inflammatory and Oxidative Markers, Epithelial Junction Proteins, and Fecal Microbiota in Rats with DNBS-Induced Colitis}},
  author = {Pakkapon Rattanachaisit and Nathawadee Lerttanatum and Somying Tumwasorn and Natthaya Chuaypen and Thasinas Dissayabutra and Duangporn Werawatganon},
  journal = {International Journal of Molecular Sciences},
  year = {2026},
  month = nov,
  doi = {10.3390/ijms27219961},
  url = {https://www.mdpi.com/1422-0067/27/21/9961},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Effects of a Two-Strain Lactobacillus Formulation (Lactobacillus rhamnosus L34 and Lactobacillus paracasei B13) on Inflammatory and Oxidative Markers, Epithelial Junction Proteins, and Fecal Microbiota in Rats with DNBS-Induced Colitis
AU  - Pakkapon Rattanachaisit
AU  - Nathawadee Lerttanatum
AU  - Somying Tumwasorn
AU  - Natthaya Chuaypen
AU  - Thasinas Dissayabutra
AU  - Duangporn Werawatganon
PY  - 2026
DA  - 2026/11/06
JO  - International Journal of Molecular Sciences
DO  - 10.3390/ijms27219961
UR  - https://www.mdpi.com/1422-0067/27/21/9961
AB  - Inflammatory bowel disease (IBD) involves dysregulated mucosal immunity, oxidative stress, impaired epithelial barrier integrity and gut microbial dysbiosis. We evaluated a two-strain formulation of Lactobacillus rhamnosus L34 and Lactobacillus paracasei B13 in DNBS-induced colitis. Twenty-four male Sprague Dawley rats were randomised into three groups (n = 8): control, acute colitis (AC; DNBS 35 mg/0.25 mL per rat rectally on days 0 and 1), and probiotic treated (RP; 3.6 × 109 CFU daily by gavage, days −3 to 6). DNBS raised Ameho histological scores, serum TNF-α, colonic MDA and MPO positivity and lowered IL-10, occludin and claudin-1 (all p < 0.05). Relative to AC, probiotic-treated rats showed lower TNF-α (p = 0.002) and MPO positivity (p < 0.001) and higher IL-10 (p = 0.001), occludin (p = 0.014) and claudin-1 (p = 0.001). Colonic MDA and Ameho scores were numerically lower in the RP group but these differences did not reach statistical significance (p = 0.085 and p = 0.310). Serum IL-17A was unchanged. Microbial community composition differed among groups (PERMANOVA pseudo-F = 5.798, R2 = 0.356, p = 0.0001), with the probiotic-associated community distinct from both control and colitis communities; distance-based analysis detected no convergence toward the control community. The formulation attenuated inflammatory and barrier outcomes and altered microbiota composition without restoring it.
SN  - 1422-0067
ER  -