Integrated Multi-Omics Analysis of Human Cerebrospinal Fluid in Traumatic Spinal Cord Injury: An Exploratory Pilot Study.
5 affiliations
- 1School of Medicine, Southeast University, Nanjing, China.
- 2Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.
- 3Department of Neurosurgery, Zhongda Hospital, Southeast University, Nanjing, China.
- 4Department of Neurosurgery, Taikang Xianlin Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China.
- 5College of Future Technology, The Hong Kong University of Science and Technology (Guangzhou), Guangzhou, China.
Abstract
Objective
Acute traumatic spinal cord injury (TSCI) is a devastating neurological disorder with limited therapeutic options. We aimed to characterize the human cerebrospinal fluid (CSF) microenvironment to identify novel multi-omics signatures and potential mechanistic hypotheses.Methods
CSF from 13 acute cervical TSCI patients and 13 controls was prospectively collected. A discovery cohort (n = 10) underwent untargeted proteomics and metabolomics. Following differential and functional enrichment analyses, sequential multi-omics integration was applied to extract cross-omics features. Prioritized candidates were subsequently validated via ELISA and targeted metabolomics in an independent validation cohort (n = 16).Results
We identified 216 differentially expressed proteins and 26 metabolites, highlighting extensive extracellular matrix remodeling alongside profound purine and amino acid metabolism dysregulation. Integrated analyses converged on downregulated mitochondrial aspartate aminotransferase (GOT2) as a candidate marker. Targeted correlation analysis revealed that GOT2 selectively associated with specific co-mapped metabolites that were concurrently downregulated, notably methionine and 4-acetamidobutanoic acid. The GOT2-associated metabolic signature was experimentally validated in the independent cohort.Conclusions
This exploratory multi-omics study unveils the acute human TSCI molecular landscape, nominating a novel GOT2-associated metabolic signature that warrants further mechanistic investigation regarding its pathogenic implications and potential therapeutic relevance.
Related
- Biochemical Biomarker Combinations in Human Traumatic Spinal Cord Injury: An Exploratory Study.
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- Personalized Management Strategies in Traumatic Spinal Cord Injury: From Resuscitation to Definitive Surgical Care
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BibTeX
@article{jiang2026integrated,
title = {{Integrated Multi-Omics Analysis of Human Cerebrospinal Fluid in Traumatic Spinal Cord Injury: An Exploratory Pilot Study.}},
author = {Wenkang Jiang and Yao Wang and Wei Miao and Huandong Liu and Qiujiang Qiao and Ting Jiang and Chenyan Shen and Zetian Zhou and Huilin Cheng and Ning Li},
journal = {CNS neuroscience \& therapeutics},
year = {2026},
month = nov,
doi = {10.1002/cns.71242},
url = {https://europepmc.org/article/MED/42969583},
note = {CC BY},
}
RIS
TY - JOUR
TI - Integrated Multi-Omics Analysis of Human Cerebrospinal Fluid in Traumatic Spinal Cord Injury: An Exploratory Pilot Study.
AU - Wenkang Jiang
AU - Yao Wang
AU - Wei Miao
AU - Huandong Liu
AU - Qiujiang Qiao
AU - Ting Jiang
AU - Chenyan Shen
AU - Zetian Zhou
AU - Huilin Cheng
AU - Ning Li
PY - 2026
DA - 2026/11/01
JO - CNS neuroscience & therapeutics
DO - 10.1002/cns.71242
UR - https://europepmc.org/article/MED/42969583
AB - Objective Acute traumatic spinal cord injury (TSCI) is a devastating neurological disorder with limited therapeutic options. We aimed to characterize the human cerebrospinal fluid (CSF) microenvironment to identify novel multi-omics signatures and potential mechanistic hypotheses.Methods CSF from 13 acute cervical TSCI patients and 13 controls was prospectively collected. A discovery cohort (n = 10) underwent untargeted proteomics and metabolomics. Following differential and functional enrichment analyses, sequential multi-omics integration was applied to extract cross-omics features. Prioritized candidates were subsequently validated via ELISA and targeted metabolomics in an independent validation cohort (n = 16).Results We identified 216 differentially expressed proteins and 26 metabolites, highlighting extensive extracellular matrix remodeling alongside profound purine and amino acid metabolism dysregulation. Integrated analyses converged on downregulated mitochondrial aspartate aminotransferase (GOT2) as a candidate marker. Targeted correlation analysis revealed that GOT2 selectively associated with specific co-mapped metabolites that were concurrently downregulated, notably methionine and 4-acetamidobutanoic acid. The GOT2-associated metabolic signature was experimentally validated in the independent cohort.Conclusions This exploratory multi-omics study unveils the acute human TSCI molecular landscape, nominating a novel GOT2-associated metabolic signature that warrants further mechanistic investigation regarding its pathogenic implications and potential therapeutic relevance.
SN - 1755-5930
SN - 1755-5949
ER -