Genome-Wide Identification and Analysis of Respiratory Burst Oxidase Homolog (RBOH) Gene Family in Nelumbo nucifera
- 1School of Horticulture and Landscape Architecture, Yangzhou University, Wenhui East Road No.48, Yangzhou 225009, China
- 2Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China
Abstract
Respiratory burst oxidase homologs (RBOHs) are core enzymes for reactive oxygen species (ROS) production in plants and play critical roles in stress signal transduction. In this study, we performed a genome-wide identification of the RBOH gene family in Nelumbo nucifera, comprehensively employed phylogenetic analysis, gene structure analysis, cis-acting element prediction, and other bioinformatics approaches, and examined their expression patterns under high-temperature stress using qRT-PCR. A total of ten NnRBOHs were identified, unevenly distributed across eight chromosomes. Phylogenetic analysis classified them into five subfamilies, with members within the same subfamily sharing highly similar gene structures and conserved motifs. The promoter regions contained abundant cis-acting elements associated with hormone, light, and stress responses. Under heat treatment, NnRBOHA and NnRBOHB were significantly upregulated, whereas the remaining NnRBOH family genes showed no significant changes in expression. These results indicate that different members of the NnRBOH family may play distinct roles during the heat-stress response. Collectively, this study clarifies the evolutionary characteristics of the NnRBOH family and identifies NnRBOHA as a potential key candidate gene for heat-tolerance genetic improvement, providing clear targets for subsequent functional validation and molecular breeding.
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BibTeX
@article{zou2026genomewide,
title = {{Genome-Wide Identification and Analysis of Respiratory Burst Oxidase Homolog (RBOH) Gene Family in Nelumbo nucifera}},
author = {Jinping Zou and Guo Ru and Yushi Zhang and Jie Zhang and Donghao Li and Kai Feng and Peng Wu and Shuping Zhao and Lei Shen and Liangjun Li},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = sep,
doi = {10.3390/ijms27188366},
url = {https://www.mdpi.com/1422-0067/27/18/8366},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Genome-Wide Identification and Analysis of Respiratory Burst Oxidase Homolog (RBOH) Gene Family in Nelumbo nucifera
AU - Jinping Zou
AU - Guo Ru
AU - Yushi Zhang
AU - Jie Zhang
AU - Donghao Li
AU - Kai Feng
AU - Peng Wu
AU - Shuping Zhao
AU - Lei Shen
AU - Liangjun Li
PY - 2026
DA - 2026/09/19
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27188366
UR - https://www.mdpi.com/1422-0067/27/18/8366
AB - Respiratory burst oxidase homologs (RBOHs) are core enzymes for reactive oxygen species (ROS) production in plants and play critical roles in stress signal transduction. In this study, we performed a genome-wide identification of the RBOH gene family in Nelumbo nucifera, comprehensively employed phylogenetic analysis, gene structure analysis, cis-acting element prediction, and other bioinformatics approaches, and examined their expression patterns under high-temperature stress using qRT-PCR. A total of ten NnRBOHs were identified, unevenly distributed across eight chromosomes. Phylogenetic analysis classified them into five subfamilies, with members within the same subfamily sharing highly similar gene structures and conserved motifs. The promoter regions contained abundant cis-acting elements associated with hormone, light, and stress responses. Under heat treatment, NnRBOHA and NnRBOHB were significantly upregulated, whereas the remaining NnRBOH family genes showed no significant changes in expression. These results indicate that different members of the NnRBOH family may play distinct roles during the heat-stress response. Collectively, this study clarifies the evolutionary characteristics of the NnRBOH family and identifies NnRBOHA as a potential key candidate gene for heat-tolerance genetic improvement, providing clear targets for subsequent functional validation and molecular breeding.
SN - 1422-0067
ER -