Rapid identification of bacteria from respiratory samples of patients hospitalized in intensive care units, with FilmArray Pneumonia Panel Plus
Abstract
Objectives This study aimed to evaluate the performance of FilmArray Pneumonia Panel Plus (FA-PP) for the detection of typical bacterial pathogens in respiratory samples from patients hospitalized in intensive care units (ICUs). Methods FA-PP was implemented for clinical use in the microbiology laboratory in March 2020. A retrospective analysis on a consecutive cohort of adult patients hospitalized in ICUs between March 2020 and May 2020 was undertaken. The respiratory samples included sputum, blind bronchoalveolar lavage (BBAL) and protected specimen brush (PSB). Conventional culture and FA-PP were performed in parallel. Results In total, 147 samples from 92 patients were analysed; 88% had coronavirus disease 2019 (COVID-19). At least one pathogen was detected in 46% (68/147) of samples by FA-PP and 39% (57/147) of samples by culture. The overall percentage agreement between FA-PP and culture results was 98% (93–100%). Bacteria with semi-quantitative FA-PP results ≥10 5 copies/mL for PSB samples, ≥10 6 copies/mL for BBAL samples and ≥10 7 copies/mL for sputum samples reached clinically significant thresholds for growth in 90%, 100% and 91% of cultures, respectively. FA-PP detected resistance markers, including mecA/ C, bla CTX-M and bla VIM . The median turnaround time was significantly shorter for FA-PP than for culture. Conclusions FA-PP may constitute a faster approach to the diagnosis of bacterial pneumonia in patients hospitalized in ICUs.
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BibTeX
@article{camelena2026rapid,
title = {{Rapid identification of bacteria from respiratory samples of patients hospitalized in intensive care units, with FilmArray Pneumonia Panel Plus}},
author = {François Caméléna and Thibaut Poncin and Emmanuel Dudoignon and Maud Salmona and Jérôme Le Goff and Jean-Luc Donay and Matthieu Lafaurie and Michael Darmon and Elie Azoulay and Benoît Plaud and Alexandre Mebazaa and François Dépret and Hervé Jacquier and Béatrice Berçot},
journal = {International Journal of Infectious Diseases},
year = {2026},
month = oct,
doi = {10.1016/j.ijid.2021.05.074},
url = {https://hal.science/hal-05783372v1},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Rapid identification of bacteria from respiratory samples of patients hospitalized in intensive care units, with FilmArray Pneumonia Panel Plus
AU - François Caméléna
AU - Thibaut Poncin
AU - Emmanuel Dudoignon
AU - Maud Salmona
AU - Jérôme Le Goff
AU - Jean-Luc Donay
AU - Matthieu Lafaurie
AU - Michael Darmon
AU - Elie Azoulay
AU - Benoît Plaud
AU - Alexandre Mebazaa
AU - François Dépret
AU - Hervé Jacquier
AU - Béatrice Berçot
PY - 2026
DA - 2026/10/07
JO - International Journal of Infectious Diseases
DO - 10.1016/j.ijid.2021.05.074
UR - https://hal.science/hal-05783372v1
AB - Objectives This study aimed to evaluate the performance of FilmArray Pneumonia Panel Plus (FA-PP) for the detection of typical bacterial pathogens in respiratory samples from patients hospitalized in intensive care units (ICUs). Methods FA-PP was implemented for clinical use in the microbiology laboratory in March 2020. A retrospective analysis on a consecutive cohort of adult patients hospitalized in ICUs between March 2020 and May 2020 was undertaken. The respiratory samples included sputum, blind bronchoalveolar lavage (BBAL) and protected specimen brush (PSB). Conventional culture and FA-PP were performed in parallel. Results In total, 147 samples from 92 patients were analysed; 88% had coronavirus disease 2019 (COVID-19). At least one pathogen was detected in 46% (68/147) of samples by FA-PP and 39% (57/147) of samples by culture. The overall percentage agreement between FA-PP and culture results was 98% (93–100%). Bacteria with semi-quantitative FA-PP results ≥10 5 copies/mL for PSB samples, ≥10 6 copies/mL for BBAL samples and ≥10 7 copies/mL for sputum samples reached clinically significant thresholds for growth in 90%, 100% and 91% of cultures, respectively. FA-PP detected resistance markers, including mecA/ C, bla CTX-M and bla VIM . The median turnaround time was significantly shorter for FA-PP than for culture. Conclusions FA-PP may constitute a faster approach to the diagnosis of bacterial pneumonia in patients hospitalized in ICUs.
SN - 1201-9712
SN - 1878-3511
ER -