Graphene Layers as Functional Biomaterials. From Fabrication and Characterization to Biological Applications
- 1National Institute of Material Physics Magurele Romania
- 2Department of Science and Engineering of Oxide Materials and Nanomaterials Faculty of Chemical Engineering and Biotechnologies University Politehnica of Bucharest Bucharest Romania
Abstract
The present study explores the influence of graphene layer number on the adhesion, proliferation, and differentiation of L‐929 fibroblast cells. Double‐ and multi‐layer graphene was fabricated using CVD on Cu and then transferred to other types of substrates by the electrochemical delamination method. Scanning electron microscopy and Raman spectroscopy confirmed the formation of a uniform and continuous graphene film. Four‐point probe measurement of double‐layer graphene revealed a higher sheet resistance. SEM and fluorescence microscopy results revealed a higher density of L‐929 cells on multi‐layer graphene, suggesting that multilayer graphene supported a significantly greater number of cells compared with double‐layer graphene. Immunofluorescence assays, focusing on vinculin and α‐SMA detection, reveal a robust cellular adhesion and extensive proliferation on the multi‐layer substrate and suggest that this has the ability to stimulate the transformation of fibroblasts into myofibroblasts, highlighting its beneficial impact on cellular function. This analysis suggests an innovative approach that demonstrates that layer‐number‐dependent structural features of pristine graphene directly modulate cell adhesion and cytoskeletal behavior, highlighting multilayer graphene as a more effective biointerface for early fibroblast activation.
Related
- Design and fabrication of silicon photonic crystals for mid-infrared sensing applications
- Fabrication and characterization of multi-shell nested fully annular quasi-ellipsoidal focusing mirrors with m=3 supermirrors for small-angle neutron scattering
- Design, Fabrication, and Free-Space Characterization of a Two-Photon-Polymerized Nano-printed Photonic Lantern
- Fabrication and characterization of lithium-diffused inverted coaxial point-contact HPGe detectors
- Fabrication and characterization of a micro-boiling meter for heat transfer measurements on a single nucleation site
BibTeX
@article{apostol2026graphene,
title = {{Graphene Layers as Functional Biomaterials. From Fabrication and Characterization to Biological Applications}},
author = {Mariana Mihaela Apostol and Issam Boukhoubza and Daniela Oprea and Iulia Corina Ciobotaru and Catalin Negrila and Ionut Enculescu and Ecaterina Andronescu and Teodor Adrian Enache and Elena Matei},
journal = {Advanced Materials Interfaces},
year = {2026},
month = nov,
doi = {10.1002/admi.70585},
url = {https://doi.org/10.1002/admi.70585},
note = {CC BY},
}
RIS
TY - JOUR
TI - Graphene Layers as Functional Biomaterials. From Fabrication and Characterization to Biological Applications
AU - Mariana Mihaela Apostol
AU - Issam Boukhoubza
AU - Daniela Oprea
AU - Iulia Corina Ciobotaru
AU - Catalin Negrila
AU - Ionut Enculescu
AU - Ecaterina Andronescu
AU - Teodor Adrian Enache
AU - Elena Matei
PY - 2026
DA - 2026/11/06
JO - Advanced Materials Interfaces
DO - 10.1002/admi.70585
UR - https://doi.org/10.1002/admi.70585
AB - The present study explores the influence of graphene layer number on the adhesion, proliferation, and differentiation of L‐929 fibroblast cells. Double‐ and multi‐layer graphene was fabricated using CVD on Cu and then transferred to other types of substrates by the electrochemical delamination method. Scanning electron microscopy and Raman spectroscopy confirmed the formation of a uniform and continuous graphene film. Four‐point probe measurement of double‐layer graphene revealed a higher sheet resistance. SEM and fluorescence microscopy results revealed a higher density of L‐929 cells on multi‐layer graphene, suggesting that multilayer graphene supported a significantly greater number of cells compared with double‐layer graphene. Immunofluorescence assays, focusing on vinculin and α‐SMA detection, reveal a robust cellular adhesion and extensive proliferation on the multi‐layer substrate and suggest that this has the ability to stimulate the transformation of fibroblasts into myofibroblasts, highlighting its beneficial impact on cellular function. This analysis suggests an innovative approach that demonstrates that layer‐number‐dependent structural features of pristine graphene directly modulate cell adhesion and cytoskeletal behavior, highlighting multilayer graphene as a more effective biointerface for early fibroblast activation.
SN - 2196-7350
ER -