Synergistic multifunctional biomedical activity of microwave-synthesized Au/Pt/Cu trimetallic nanofluids: experimental and DFT insights
8 affiliations
- 1Department of Physics, University of Allahabad Prayagraj-211002 Uttar Pradesh India
- 2Department of Chemistry, Swansea University Singleton Park Campus Swansea SA2 8PP UK
- 3Center of Agriculture, Food, Environment, University of Trento San Michele all’Adige 38010 Italy
- 4Department of Chemistry, Guru Jambheshwar University of Science and Technology Hisar-125001 India
- 5Department of Chemistry, Baba Raghav Das Post Graduate College Deoria-274001 Uttar Pradesh India
- 6Institute of Pharmaceutical Science, King's College London Franklin-Wilkins Building, Stamford Street SE1 9NH London UK
- 7Department of Chemical Engineering, Swansea University Bay Campus Swansea SA1 8EN UK
- 8Department of Chemistry, University of Lucknow Lucknow-226007 Uttar Pradesh India
Abstract
In the present study, a trimetallic Au/Pt/Cu nanofluid was synthesized via a rapid one-pot microwave-assisted strategy and systematically evaluated for multifunctional biomedical activity. Structural and spectroscopic characterization confirmed the formation of an integrated multimetallic nanostructure, likely to contain alloyed or compositionally mixed domains that generate synergistically active interfaces of Au, Pt and Cu. The nanofluid exhibited broad-spectrum antimicrobial activity, inhibiting Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis) and Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa) with a minimum inhibitory concentration (MIC) of 0.0137 µmol mL−1. Enhanced antifungal efficacy was observed against Rhizopus oryzae and Candida albicans (MIC = 0.0068 µmol mL−1), with activity against C. albicans surpassing that of fluconazole under identical conditions. The anti-inflammatory potential evaluated through bovine serum albumin denaturation revealed an IC50 of 14.88 ± 0.09 µM, while antioxidant capacity measured using the DPPH assay showed an IC50 of 4.34 ± 0.08 µM, indicating effective free-radical scavenging in the low-micromolar range. These findings highlight a synergy-driven mechanism integrating catalytic redox modulation, biomolecular interactions, and enhanced physicochemical compatibility.
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BibTeX
@article{yadav2026synergistic,
title = {{Synergistic multifunctional biomedical activity of microwave-synthesized Au/Pt/Cu trimetallic nanofluids: experimental and DFT insights}},
author = {Navneet Yadav and Amara Rafi and Lalan Kumar and Binesh Kumar and Sikandar Paswan and Ajit Kumar Maddheshiya and Jai Devi and Nikunj Patel and Afsanehsadat Larimi and Bharat Kumar},
journal = {RSC Advances},
year = {2026},
month = oct,
doi = {10.1039/d6ra06054d},
url = {https://doi.org/10.1039/d6ra06054d},
note = {CC BY 3.0},
}
RIS
TY - JOUR
TI - Synergistic multifunctional biomedical activity of microwave-synthesized Au/Pt/Cu trimetallic nanofluids: experimental and DFT insights
AU - Navneet Yadav
AU - Amara Rafi
AU - Lalan Kumar
AU - Binesh Kumar
AU - Sikandar Paswan
AU - Ajit Kumar Maddheshiya
AU - Jai Devi
AU - Nikunj Patel
AU - Afsanehsadat Larimi
AU - Bharat Kumar
PY - 2026
DA - 2026/10/09
JO - RSC Advances
DO - 10.1039/d6ra06054d
UR - https://doi.org/10.1039/d6ra06054d
AB - In the present study, a trimetallic Au/Pt/Cu nanofluid was synthesized via a rapid one-pot microwave-assisted strategy and systematically evaluated for multifunctional biomedical activity. Structural and spectroscopic characterization confirmed the formation of an integrated multimetallic nanostructure, likely to contain alloyed or compositionally mixed domains that generate synergistically active interfaces of Au, Pt and Cu. The nanofluid exhibited broad-spectrum antimicrobial activity, inhibiting Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis) and Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa) with a minimum inhibitory concentration (MIC) of 0.0137 µmol mL−1. Enhanced antifungal efficacy was observed against Rhizopus oryzae and Candida albicans (MIC = 0.0068 µmol mL−1), with activity against C. albicans surpassing that of fluconazole under identical conditions. The anti-inflammatory potential evaluated through bovine serum albumin denaturation revealed an IC50 of 14.88 ± 0.09 µM, while antioxidant capacity measured using the DPPH assay showed an IC50 of 4.34 ± 0.08 µM, indicating effective free-radical scavenging in the low-micromolar range. These findings highlight a synergy-driven mechanism integrating catalytic redox modulation, biomolecular interactions, and enhanced physicochemical compatibility.
SN - 2046-2069
ER -