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Peer-reviewedCC BY 4.0ACS Omega

Valorization of Cannabis sativa Residual Biomass from Industrial CBD Extraction as a Renewable Support for Candida antarctica Lipase B Immobilization and Fatty Acid Esterification

  • Giorgia Albonetti1
  • Gabrielle A. R. da Silva2
  • Matheus C. da Silva2
  • Caio M. Pacheco2
  • Raquel A. C. Leão2
  • Alessandro Massi1
  • Alessandro Pellis3
  • Omar G. Pandoli4,5
  • Ivaldo Itabaiana6
  • Rodrigo O. M. A. de Souza2
6 affiliations
  1. 1University of Ferrara , , Via L. Borsari, 46 , ,
  2. 2Federal University of Rio de Janeiro , , , ,
  3. 3University of Genoa , , Via Dodecaneso, 31 , ,
  4. 4Università degli Studi di Genova , , Viale Cembrano 4 , ,
  5. 5Istituto Italiano di Tecnologia (IIT) , Via Morego 30 , ,
  6. 6Federal University of Rio de Janeiro , , ,
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Abstract

Residual Cannabis sativa biomass from industrial CBD extraction in ethanol was evaluated as a renewable support for the immobilization of Candida antarctica lipase B (CALB) by physical adsorption, and the resulting biocatalysts were applied to the esterification of long-chain fatty acids with ethanol. Because extractives released by the untreated biomass interfered with protein quantification, three simple pretreatments were compared. Milling alone, with or without buffer washing, gave reproducible immobilization and active, reusable biocatalysts, whereas SDS washing increased protein retention but led to nearly inactive preparations, indicating that protein loading alone does not predict catalytic performance. The milling-only biocatalyst converted two industrial palm fatty acid distillate fractions with conversions comparable to those of Novozym 435, retained more than 80% of its initial conversion after five cycles, and was operated in a continuous-flow packed-bed reactor with conversions of 95% or higher at a residence time of 15 min. These results indicate that this residue is a low-cost, minimally processed support for lipase-catalyzed esterification.

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Cite

BibTeXRIS
BibTeX
@article{albonetti2026valorization,
  title = {{Valorization of Cannabis sativa Residual Biomass from Industrial CBD Extraction as a Renewable Support for Candida antarctica Lipase B Immobilization and Fatty Acid Esterification}},
  author = {Giorgia Albonetti and Gabrielle A. R. da Silva and Matheus C. da Silva and Caio M. Pacheco and Raquel A. C. Leão and Alessandro Massi and Alessandro Pellis and Omar G. Pandoli and Ivaldo Itabaiana and Rodrigo O. M. A. de Souza},
  journal = {ACS Omega},
  year = {2026},
  month = nov,
  doi = {10.1021/acsomega.6c10952},
  url = {https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c10952/5463399/Valorization-of-Cannabis-sativa-Residual-Biomass},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Valorization of Cannabis sativa Residual Biomass from Industrial CBD Extraction as a Renewable Support for Candida antarctica Lipase B Immobilization and Fatty Acid Esterification
AU  - Giorgia Albonetti
AU  - Gabrielle A. R. da Silva
AU  - Matheus C. da Silva
AU  - Caio M. Pacheco
AU  - Raquel A. C. Leão
AU  - Alessandro Massi
AU  - Alessandro Pellis
AU  - Omar G. Pandoli
AU  - Ivaldo Itabaiana
AU  - Rodrigo O. M. A. de Souza
PY  - 2026
DA  - 2026/11/04
JO  - ACS Omega
DO  - 10.1021/acsomega.6c10952
UR  - https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c10952/5463399/Valorization-of-Cannabis-sativa-Residual-Biomass
AB  - Residual Cannabis sativa biomass from industrial CBD extraction in ethanol was evaluated as a renewable support for the immobilization of Candida antarctica lipase B (CALB) by physical adsorption, and the resulting biocatalysts were applied to the esterification of long-chain fatty acids with ethanol. Because extractives released by the untreated biomass interfered with protein quantification, three simple pretreatments were compared. Milling alone, with or without buffer washing, gave reproducible immobilization and active, reusable biocatalysts, whereas SDS washing increased protein retention but led to nearly inactive preparations, indicating that protein loading alone does not predict catalytic performance. The milling-only biocatalyst converted two industrial palm fatty acid distillate fractions with conversions comparable to those of Novozym 435, retained more than 80% of its initial conversion after five cycles, and was operated in a continuous-flow packed-bed reactor with conversions of 95% or higher at a residence time of 15 min. These results indicate that this residue is a low-cost, minimally processed support for lipase-catalyzed esterification.
SN  - 2470-1343
ER  -