Two closely related RNA helicases, UAP56 and URH49, couple mRNA splicing and export pathways with their distinct complexes
- 1Faculty of Agriculture Kindai University
Abstract
Transcription and mRNA processing are integrated into appropriate mRNA export pathway(s) enabling fine-tuned gene expression. The closely related RNA helicases, UAP56 and URH49, form distinct complexes known as the apo-TREX and the apo-AREX complexes, respectively, which remodel into the ATP-TREX complexes, thus forming multiple mRNA export pathways. However, the mechanisms by which helicases and their complexes shape these pathways remain unclear. Here, we showed that UAP56 and URH49 play general roles in mRNA splicing ahead of export. Further analyses revealed three patterns of splicing regulation and their association with mRNA export: Type I, in which both helicases redundantly or synergistically regulate splicing within the same transcripts, associated with cooperative mRNA export; Type II, in which either helicase preferentially regulates splicing in distinct transcripts; and Type III, in which the helicases exert opposing splicing effects within the same transcripts. Type II and III regulation was associated with selective mRNA export. Moreover, we demonstrated that the distinct complex formation influenced the splicing patterns regulated by the two helicases. Our findings reveal an additional layer of transcriptome regulation mediated by UAP56, URH49, and their complexes, extending beyond their canonical roles in mRNA export.
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BibTeX
@misc{fujita2026closely,
title = {{Two closely related RNA helicases, UAP56 and URH49, couple mRNA splicing and export pathways with their distinct complexes}},
author = {K.-i. Fujita and K. Harada and Y. Ikeda and H. Yoshioka and M. Yokoshi and F. Hori and N. Fujiwara and S. Seno and H. Matsuda and Y. Kawahara and A. Mayeda and S. Masuda},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.27.762353},
url = {https://www.biorxiv.org/content/10.64898/2026.10.27.762353v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Two closely related RNA helicases, UAP56 and URH49, couple mRNA splicing and export pathways with their distinct complexes
AU - K.-i. Fujita
AU - K. Harada
AU - Y. Ikeda
AU - H. Yoshioka
AU - M. Yokoshi
AU - F. Hori
AU - N. Fujiwara
AU - S. Seno
AU - H. Matsuda
AU - Y. Kawahara
AU - A. Mayeda
AU - S. Masuda
PY - 2026
DA - 2026/10/27
PB - bioRxiv
DO - 10.64898/2026.10.27.762353
UR - https://www.biorxiv.org/content/10.64898/2026.10.27.762353v1
AB - Transcription and mRNA processing are integrated into appropriate mRNA export pathway(s) enabling fine-tuned gene expression. The closely related RNA helicases, UAP56 and URH49, form distinct complexes known as the apo-TREX and the apo-AREX complexes, respectively, which remodel into the ATP-TREX complexes, thus forming multiple mRNA export pathways. However, the mechanisms by which helicases and their complexes shape these pathways remain unclear. Here, we showed that UAP56 and URH49 play general roles in mRNA splicing ahead of export. Further analyses revealed three patterns of splicing regulation and their association with mRNA export: Type I, in which both helicases redundantly or synergistically regulate splicing within the same transcripts, associated with cooperative mRNA export; Type II, in which either helicase preferentially regulates splicing in distinct transcripts; and Type III, in which the helicases exert opposing splicing effects within the same transcripts. Type II and III regulation was associated with selective mRNA export. Moreover, we demonstrated that the distinct complex formation influenced the splicing patterns regulated by the two helicases. Our findings reveal an additional layer of transcriptome regulation mediated by UAP56, URH49, and their complexes, extending beyond their canonical roles in mRNA export.
ER -