Thylakoid Nanoarchitecture and Photosystem II Energy Partitioning in Response to Treatment with Huminatrin Concentrate “Boron”
5 affiliations
- 1Kirensky Institute of Physics FRC «KSC SB RAS», Academgorodok Str. 50/12, Krasnoyarsk 660036, Russia
- 2Federal Research Center «KSC of SB RAS», Academgorodok Str. 50, Krasnoyarsk 660036, Russia
- 3School of Fundamental Biology and Biotechnology, Siberian Federal University, 79, Svobodny Str., Krasnoyarsk 660041, Russia
- 4Krasnoyarsk Agricultural Research Institute, FRC «KSC SB RAS», Prosp. Svobodnyi, 66, Krasnoyarsk 660041, Russia
- 5Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk 660036, Russia
Abstract
Photosynthetic acclimation involves coordinated changes in the structural organization and functional activity of the photosynthetic apparatus. However, the structural basis underlying enhanced photosynthetic activity following biostimulant treatment under non-stress conditions remains insufficiently understood. In the present study, we investigated the effects of exogenous application of the humic-boron fertilizer Huminatrin Concentrate “Boron” on the structural and functional organization of the photosynthetic apparatus in spring rapeseed (Brassica napus L.) cv. Siberian. Thylakoid and granum architecture was characterized using transmission electron microscopy and quantitative morphometric analysis, whereas photosynthetic function was assessed using pulse-amplitude-modulated (PAM) chlorophyll fluorescence. Huminatrin treatment was associated with changes in granum organization, including a reduction in the stacking repeat distance (SRD), as well as increases in the apparent electron transport rate (ETR) and the effective quantum yield of photosystem II (PSII) photochemistry (Y(II)). Redistribution of PSII excitation energy was accompanied by a decrease in the regulatory quantum yield in PSII (Y(NPQ)) and an increase in the non-regulated energy loss in PSII (Y(NO)). These changes occurred without evidence of pronounced PSII photoinhibition, indicating that the observed changes in PSII photochemistry were not a response to experimentally induced stress. Structural and functional changes were accompanied by increases in plant and leaf biomass. Taken together, these findings indicate that leaf biostimulation is associated with coordinated remodeling of thylakoid architecture and PSII excitation-energy partitioning under the experimental conditions studied.
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BibTeX
@article{bukhanov2026thylakoid,
title = {{Thylakoid Nanoarchitecture and Photosystem II Energy Partitioning in Response to Treatment with Huminatrin Concentrate “Boron”}},
author = {Eugene Romanovich Bukhanov and Nikolay Alexandrovich Gaevsky and Olga Alexandrovna Poznakhareva and Alexander Apollinaryevich Tikhomirov and Sofya Avrumovna Ushakova and Vasily Filippovich Shabanov},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = oct,
doi = {10.3390/ijms27219664},
url = {https://www.mdpi.com/1422-0067/27/21/9664},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Thylakoid Nanoarchitecture and Photosystem II Energy Partitioning in Response to Treatment with Huminatrin Concentrate “Boron”
AU - Eugene Romanovich Bukhanov
AU - Nikolay Alexandrovich Gaevsky
AU - Olga Alexandrovna Poznakhareva
AU - Alexander Apollinaryevich Tikhomirov
AU - Sofya Avrumovna Ushakova
AU - Vasily Filippovich Shabanov
PY - 2026
DA - 2026/10/28
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27219664
UR - https://www.mdpi.com/1422-0067/27/21/9664
AB - Photosynthetic acclimation involves coordinated changes in the structural organization and functional activity of the photosynthetic apparatus. However, the structural basis underlying enhanced photosynthetic activity following biostimulant treatment under non-stress conditions remains insufficiently understood. In the present study, we investigated the effects of exogenous application of the humic-boron fertilizer Huminatrin Concentrate “Boron” on the structural and functional organization of the photosynthetic apparatus in spring rapeseed (Brassica napus L.) cv. Siberian. Thylakoid and granum architecture was characterized using transmission electron microscopy and quantitative morphometric analysis, whereas photosynthetic function was assessed using pulse-amplitude-modulated (PAM) chlorophyll fluorescence. Huminatrin treatment was associated with changes in granum organization, including a reduction in the stacking repeat distance (SRD), as well as increases in the apparent electron transport rate (ETR) and the effective quantum yield of photosystem II (PSII) photochemistry (Y(II)). Redistribution of PSII excitation energy was accompanied by a decrease in the regulatory quantum yield in PSII (Y(NPQ)) and an increase in the non-regulated energy loss in PSII (Y(NO)). These changes occurred without evidence of pronounced PSII photoinhibition, indicating that the observed changes in PSII photochemistry were not a response to experimentally induced stress. Structural and functional changes were accompanied by increases in plant and leaf biomass. Taken together, these findings indicate that leaf biostimulation is associated with coordinated remodeling of thylakoid architecture and PSII excitation-energy partitioning under the experimental conditions studied.
SN - 1422-0067
ER -