Skip to content
DraconiticResearch
Search Sources API
203,234 studies31 sourcesUpdated 5 min ago
Peer-reviewedCC BY-NC-NDJournal of Isotopes

Synthesis of Stable Isotope Labeled Benzidine-D8

  • Zhenjie CHEN1
  • Zhongjie XU1
  • Ruoning FAN1
  1. 1Biomedical Testing Center, Shanghai Research Institute of Chemical Industry Co., Ltd, Shanghai 200062, China
Read full textDOICite

Abstract

Aiming at the shortage of benzidine isotope internal standard reagents in environmental monitoring and food safety detection，an efficient synthetic method for stable isotope deuterium-labeled benzidine-D8 was developed. Benzidine-D8 was synthesized via two-step reactions: reduction of nitrobenzene-D5 to hydrazobenzene-D10 under alkaline conditions with zinc powder，followed by acid-catalyzed intramolecular rearrangement. The total yield of benzidine-D8 was 50.3% based on the molar amount of starting material nitrobenzene-D5. The product was characterized by HPLC and ESI-MS，with a chemical purity of 99.7% and deuterium isotopic abundance of 99.8 atom%. The results indicate that the synthesized benzidine-D8 is suitable as an internal standard for the quantitative determination of benzidine residues in soil，water and textile samples.

Related

  1. Synthesis and Characterization of Stable Isotope Labeled 2,3,3’,4,4’,5-Hexachlorobiphenyl-2’,6,6’-D3
    Rong WANG, Hui GUO, Wulian CHEN
    Journal of IsotopesPeer-reviewedCC BY-NC-NDFull textPharmacology & toxicologyDOAJ
  2. Stable isotope‐derived measurements of protein synthesis detect amino acid‐induced anabolism in skeletal muscle microtissues not captured by puromycin labelling
    Yekaterina Tiper, Cassidy T. Tinline‐Goodfellow, Penney M. Gilbert +1
    Experimental physiologyPeer-reviewedCC BYFull textGeneral medicineEurope PMC
  3. Identification of stress from incremental dentine stable isotope trends using linear enamel hypoplasia
    Emma Smith, Angela Lamb, Linda Fibiger +3
    PLOS OnePeer-reviewedCC BY 4.0Full textNutritionPhysiologyPLOS
  4. Evaluation of peroxidase activity by alpha-naphthol/pyronine staining compared with benzidine staining in 101 acute leukemia cases.
    Veronique Latger-Cannard, Valérie Bardet, M Malet +3
    Laboratory hematology : official publication of the International Society for Laboratory HematologyPeer-reviewedCC BY3 citedGeneral biologyGeneral medicineHAL
  5. A Bench-Stable Reagent for Divergent Synthesis and Storage of SF5X
    Lujie Xu, Richard Kraus, David Rombach
    JACS AuPeer-reviewedCC BY 4.0Full textPharmacology & toxicologyCrossref

Cite

BibTeXRIS
BibTeX
@article{chen2026synthesis,
  title = {{Synthesis of Stable Isotope Labeled Benzidine-D8}},
  author = {Zhenjie CHEN and Zhongjie XU and Ruoning FAN},
  journal = {Journal of Isotopes},
  year = {2026},
  month = oct,
  doi = {10.7538/tws.2026.youxian.053},
  url = {https://doi.org/10.7538/tws.2026.youxian.053},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - Synthesis of Stable Isotope Labeled Benzidine-D8
AU  - Zhenjie CHEN
AU  - Zhongjie XU
AU  - Ruoning FAN
PY  - 2026
DA  - 2026/10/26
JO  - Journal of Isotopes
DO  - 10.7538/tws.2026.youxian.053
UR  - https://doi.org/10.7538/tws.2026.youxian.053
AB  - Aiming at the shortage of benzidine isotope internal standard reagents in environmental monitoring and food safety detection，an efficient synthetic method for stable isotope deuterium-labeled benzidine-D8 was developed. Benzidine-D8 was synthesized via two-step reactions: reduction of nitrobenzene-D5 to hydrazobenzene-D10 under alkaline conditions with zinc powder，followed by acid-catalyzed intramolecular rearrangement. The total yield of benzidine-D8 was 50.3% based on the molar amount of starting material nitrobenzene-D5. The product was characterized by HPLC and ESI-MS，with a chemical purity of 99.7% and deuterium isotopic abundance of 99.8 atom%. The results indicate that the synthesized benzidine-D8 is suitable as an internal standard for the quantitative determination of benzidine residues in soil，water and textile samples.
SN  - 1000-7512
ER  -