Membrane orientation regulates GAPR-1 oligomerization that is suitable for interaction with beclin 1
- 1Utrecht, The Netherlands
- 2Bristol, BS8 1TD UK
- 3Coventry, CV4 7AL UK
Abstract
Golgi-associated plant pathogenesis-related protein 1 (GAPR-1) is a beclin 1-interacting peripheral membrane protein that is anchored to the Golgi complex via an N-myristoyl-group and ionic interactions. Here we investigated the determinants of GAPR-1 membrane binding using molecular dynamics (MD) simulations and applied the results to investigate the subsequent oligomerization into biomolecular condensates and interaction with beclin 1. MD simulations revealed a specific orientation of GAPR-1 upon docking to a negatively charged lipid bilayer involving lysines at position 7 and 33 (Lys7 and Lys33). Substitution of these lysines with glutamine changed the preferred membrane orientation of GAPR-1. Upon deletion of the myristoyl-group, GAPR-1 remained docked to the lipid bilayer, but lost its preferred orientation, suggesting that myristoylation constrained the dynamics of the protein at the membrane surface. Upon mutation of Lys7 to glutamine and expression in Saccharomyces cerevisiae, [K7Q]GAPR-1 was prevented from myristoylation and condensate formation was severely impaired. Similar results were obtained when N-terminal myristoylation of GAPR-1 was prevented by mutating amino acid Gly2. However, in contrast to [G2A]GAPR-1, [K7Q]GAPR-1 could not interact with beclin 1 anymore upon co-expression in yeast cells, and failed to interfere with beclin 1-dependent autophagy in human cells. Our combined data show that GAPR-1 requires a defined orientation at the membrane to control protein homo-and hetero-oligomerization and to execute its function. We revealed a novel function of protein myristoylation in which it determines the motional freedom of specific protein orientations, which may have relevance for the entire myristoylated proteome.
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BibTeX
@article{shen2026membrane,
title = {{Membrane orientation regulates GAPR-1 oligomerization that is suitable for interaction with beclin 1}},
author = {Ziying Shen and Robin A. Corey and Hidde van Doorne and Iris Verhoek and Renée Koopman and Daniel S. Huitema and Samiksha Sardana and Marc P. Baggelaar and Phillip J. Stansfeld and Dora V. Kaloyanova and J. Bernd Helms},
journal = {Cellular and molecular life sciences : CMLS},
year = {2026},
month = jul,
doi = {10.1007/s00018-026-06329-4},
url = {https://europepmc.org/article/PMC/PMC13690689},
note = {CC BY},
}
RIS
TY - JOUR
TI - Membrane orientation regulates GAPR-1 oligomerization that is suitable for interaction with beclin 1
AU - Ziying Shen
AU - Robin A. Corey
AU - Hidde van Doorne
AU - Iris Verhoek
AU - Renée Koopman
AU - Daniel S. Huitema
AU - Samiksha Sardana
AU - Marc P. Baggelaar
AU - Phillip J. Stansfeld
AU - Dora V. Kaloyanova
AU - J. Bernd Helms
PY - 2026
DA - 2026/07/01
JO - Cellular and molecular life sciences : CMLS
DO - 10.1007/s00018-026-06329-4
UR - https://europepmc.org/article/PMC/PMC13690689
AB - Golgi-associated plant pathogenesis-related protein 1 (GAPR-1) is a beclin 1-interacting peripheral membrane protein that is anchored to the Golgi complex via an N-myristoyl-group and ionic interactions. Here we investigated the determinants of GAPR-1 membrane binding using molecular dynamics (MD) simulations and applied the results to investigate the subsequent oligomerization into biomolecular condensates and interaction with beclin 1. MD simulations revealed a specific orientation of GAPR-1 upon docking to a negatively charged lipid bilayer involving lysines at position 7 and 33 (Lys7 and Lys33). Substitution of these lysines with glutamine changed the preferred membrane orientation of GAPR-1. Upon deletion of the myristoyl-group, GAPR-1 remained docked to the lipid bilayer, but lost its preferred orientation, suggesting that myristoylation constrained the dynamics of the protein at the membrane surface. Upon mutation of Lys7 to glutamine and expression in Saccharomyces cerevisiae, [K7Q]GAPR-1 was prevented from myristoylation and condensate formation was severely impaired. Similar results were obtained when N-terminal myristoylation of GAPR-1 was prevented by mutating amino acid Gly2. However, in contrast to [G2A]GAPR-1, [K7Q]GAPR-1 could not interact with beclin 1 anymore upon co-expression in yeast cells, and failed to interfere with beclin 1-dependent autophagy in human cells. Our combined data show that GAPR-1 requires a defined orientation at the membrane to control protein homo-and hetero-oligomerization and to execute its function. We revealed a novel function of protein myristoylation in which it determines the motional freedom of specific protein orientations, which may have relevance for the entire myristoylated proteome.
SN - 1420-682X
SN - 1420-9071
ER -