Hybrid QuEChERS–Micro-Solid Phase Extraction for the Determination of Chloramphenicol in Honey by HPLC-MS/MS: Method Validation and Matrix-Effect Assessment
- 1Department of Chemical Research of Food and Feed, National Veterinary Research Institute, 24-100 Pulawy, Poland
Abstract
An HPLC-MS/MS method combining modified QuEChERS-based extraction with micro-solid-phase extraction (MSPE) using C18P-PVDF microspin filters was developed and validated for chloramphenicol (CAP) determination in honey. At validation levels of 0.10, 0.15, and 0.30 µg kg−1, apparent recoveries ranged from 102.1% to 105.1%, repeatability (RSDr) from 4.5% to 9.5%, and within-laboratory reproducibility (RSDwR) from 6.0% to 12.5%. The decision limit (CCα) was 0.123 µg kg−1, below the reference point for action of 0.15 µg kg−1, while the estimated limit of detection (LOD; not experimentally verified) was 0.01 µg kg−1.. Matrix-effect assessment across 20 honey samples showed responses within the 80–120% range for the proposed procedure and the SPE and QuEChERS comparators. No CAP was detected in the authentic honey samples. The developed workflow provides a practical, validated alternative for routine CAP determination in honey.
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BibTeX
@article{sniegocki2026hybrid,
title = {{Hybrid QuEChERS–Micro-Solid Phase Extraction for the Determination of Chloramphenicol in Honey by HPLC-MS/MS: Method Validation and Matrix-Effect Assessment}},
author = {Tomasz Śniegocki and Elżbieta Samorek and Weronika Krajewska},
journal = {Molecules},
year = {2026},
month = nov,
doi = {10.3390/molecules31213971},
url = {https://www.mdpi.com/1420-3049/31/21/3971},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Hybrid QuEChERS–Micro-Solid Phase Extraction for the Determination of Chloramphenicol in Honey by HPLC-MS/MS: Method Validation and Matrix-Effect Assessment
AU - Tomasz Śniegocki
AU - Elżbieta Samorek
AU - Weronika Krajewska
PY - 2026
DA - 2026/11/04
JO - Molecules
DO - 10.3390/molecules31213971
UR - https://www.mdpi.com/1420-3049/31/21/3971
AB - An HPLC-MS/MS method combining modified QuEChERS-based extraction with micro-solid-phase extraction (MSPE) using C18P-PVDF microspin filters was developed and validated for chloramphenicol (CAP) determination in honey. At validation levels of 0.10, 0.15, and 0.30 µg kg−1, apparent recoveries ranged from 102.1% to 105.1%, repeatability (RSDr) from 4.5% to 9.5%, and within-laboratory reproducibility (RSDwR) from 6.0% to 12.5%. The decision limit (CCα) was 0.123 µg kg−1, below the reference point for action of 0.15 µg kg−1, while the estimated limit of detection (LOD; not experimentally verified) was 0.01 µg kg−1.. Matrix-effect assessment across 20 honey samples showed responses within the 80–120% range for the proposed procedure and the SPE and QuEChERS comparators. No CAP was detected in the authentic honey samples. The developed workflow provides a practical, validated alternative for routine CAP determination in honey.
SN - 1420-3049
ER -