Prime-Editing in Marchantia paleacea : Expanding the Genome-Editing Toolbox in Bryophytes

Abstract

Since the development of CRISPR-based genome editing tools, a number of novel technologies have emerged. This includes Prime-Editing that acts as a search and replace genome editing tool. Prime-Editing has been deployed across multiple clades, including in a few flowering plants. Here, we report on the development of an efficient Prime Editor (PE) for the model bryophyte Marchantia. Initial tests were conducted on Acetolactate Synthase as a target and revealed an average efficiency above 40%. The system has been developed in the GoldenGate cloning system, facilitating construct design. The development of PE in Marchantia expands the Genome-Editing tools available for this emerging model in plant biology.

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BibTeX RIS

BibTeX

@misc{danilo2026primeediting,
  title = {{Prime-Editing in Marchantia paleacea : Expanding the Genome-Editing Toolbox in Bryophytes}},
  author = {Benoit Danilo and Aurélie Quillien and Cristina Rojas-Latorre and Zaïd Nibani and Clara Mestre and Pierre-Marc Delaux and Dominique Lauressergues and Julie Neveu},
  howpublished = {HAL},
  year = {2026},
  month = aug,
  doi = {10.64898/2026.08.07.743462},
  url = {https://hal.science/hal-05782767v1},
  note = {CC BY-NC},
}

RIS

TY  - UNPB
TI  - Prime-Editing in Marchantia paleacea : Expanding the Genome-Editing Toolbox in Bryophytes
AU  - Benoit Danilo
AU  - Aurélie Quillien
AU  - Cristina Rojas-Latorre
AU  - Zaïd Nibani
AU  - Clara Mestre
AU  - Pierre-Marc Delaux
AU  - Dominique Lauressergues
AU  - Julie Neveu
PY  - 2026
DA  - 2026/08/11
PB  - HAL
DO  - 10.64898/2026.08.07.743462
UR  - https://hal.science/hal-05782767v1
AB  - Since the development of CRISPR-based genome editing tools, a number of novel technologies have emerged. This includes Prime-Editing that acts as a search and replace genome editing tool. Prime-Editing has been deployed across multiple clades, including in a few flowering plants. Here, we report on the development of an efficient Prime Editor (PE) for the model bryophyte Marchantia. Initial tests were conducted on Acetolactate Synthase as a target and revealed an average efficiency above 40%. The system has been developed in the GoldenGate cloning system, facilitating construct design. The development of PE in Marchantia expands the Genome-Editing tools available for this emerging model in plant biology.
ER  -

Abstract

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