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Peer-reviewedCC BY-NC-ND 4.0Virologica Sinica

SwiVirScan, a high-throughput platform for profiling antibodies to swine-associated viruses, reveals extensive viral exposure histories in pigs

  • Qian Zhang1,2,3
  • Junhua Dong1,2,3
  • Pengju Guo1,2,3
  • Wenlong Wang1,2,3
  • Xueting Zhang1,2,3
  • Siyang Pei1,2,3
  • Zhaoxu Dong1,2,3
  • Ping Qian1,2,3
  • Pan Tao1,2,3
  1. 1State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China
  2. 2Hubei Hongshan Lab, Wuhan, 430070, China
  3. 3Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China
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Abstract

Pigs are crucial as food sources, biomedical models, and reservoirs of some zoonotic viruses, yet their comprehensive viral exposure history remains poorly understood. Here, we developed SwiVirScan, a T7 phage display-based serological platform containing 36,416 peptides derived from 140 known swine-associated viruses. Application of SwiVirScan to 127 field serum samples from adult breeding pigs revealed extensive and diverse antibody signatures: every sample recognized peptides assigned to multiple viruses, including viruses with reported zoonotic relevance or potential for cross-species transmission. Pairwise analysis identified enriched co-seropositivity patterns, including a robust association between porcine bufavirus (PBuV) and porcine circovirus 3 (PCV3), and uncovered distinct associations between seroreactivity to vaccine-targeted viruses and other viral exposures. These findings establish SwiVirScan as a high-throughput platform for mapping viral exposure histories in pig populations and prioritizing viral targets for focused surveillance and investigation.

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Cite

BibTeXRIS
BibTeX
@article{zhang2026swivirscan,
  title = {{SwiVirScan, a high-throughput platform for profiling antibodies to swine-associated viruses, reveals extensive viral exposure histories in pigs}},
  author = {Qian Zhang and Junhua Dong and Pengju Guo and Wenlong Wang and Xueting Zhang and Siyang Pei and Zhaoxu Dong and Ping Qian and Pan Tao},
  journal = {Virologica Sinica},
  year = {2026},
  month = sep,
  doi = {10.1016/j.virs.2026.09.013},
  url = {https://doi.org/10.1016/j.virs.2026.09.013},
  note = {CC BY-NC-ND 4.0},
}
RIS
TY  - JOUR
TI  - SwiVirScan, a high-throughput platform for profiling antibodies to swine-associated viruses, reveals extensive viral exposure histories in pigs
AU  - Qian Zhang
AU  - Junhua Dong
AU  - Pengju Guo
AU  - Wenlong Wang
AU  - Xueting Zhang
AU  - Siyang Pei
AU  - Zhaoxu Dong
AU  - Ping Qian
AU  - Pan Tao
PY  - 2026
DA  - 2026/09/30
JO  - Virologica Sinica
DO  - 10.1016/j.virs.2026.09.013
UR  - https://doi.org/10.1016/j.virs.2026.09.013
AB  - Pigs are crucial as food sources, biomedical models, and reservoirs of some zoonotic viruses, yet their comprehensive viral exposure history remains poorly understood. Here, we developed SwiVirScan, a T7 phage display-based serological platform containing 36,416 peptides derived from 140 known swine-associated viruses. Application of SwiVirScan to 127 field serum samples from adult breeding pigs revealed extensive and diverse antibody signatures: every sample recognized peptides assigned to multiple viruses, including viruses with reported zoonotic relevance or potential for cross-species transmission. Pairwise analysis identified enriched co-seropositivity patterns, including a robust association between porcine bufavirus (PBuV) and porcine circovirus 3 (PCV3), and uncovered distinct associations between seroreactivity to vaccine-targeted viruses and other viral exposures. These findings establish SwiVirScan as a high-throughput platform for mapping viral exposure histories in pig populations and prioritizing viral targets for focused surveillance and investigation.
SN  - 1674-0769
SN  - 1995-820X
ER  -