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Peer-reviewedCC BY 4.0Advanced Science

Matrix Stiffness Enhances Odontogenic Differentiation of DPSCs through Membrane Curvature Protein Baiap2‐Modulated Exosome Release

  • Bilun Jin1,2,3
  • Shaojie Dong1,2,3
  • Yue Jia1,2,3
  • Zhao Xu4,5
  • Peiqi Liu1,2,3
  • Zhaojing Ding1,2,3
  • Yuxin Liao1,2,3
  • Rui Zou1,2,3
  • Jinsong Hu6
  • Bo Cheng4,5
  • Lin Niu1,2,3
6 affiliations
  1. 1Key Laboratory of Shaanxi Province For Craniofacial Precision Medicine Research, College of Stomatology Xi'an Jiaotong University Xi'an China
  2. 2Clinical Research Center of Shaanxi Province For Dental and Maxillofacial Diseases, College of Stomatology Xi'an Jiaotong University Xi'an China
  3. 3College of Stomatology Xi'an Jiaotong University Xi'an China
  4. 4School of Life Science and Technology The Key Laboratory of Biomedical Information Engineering of the Ministry of Education Xi'an Jiaotong University Xi'an China
  5. 5Bioinspired Engineering and Biomechanics Center (BEBC) Xi'an Jiaotong University Xi'an China
  6. 6Department of Cell Biology and Genetics School of Basic Medical Sciences Xi'an Jiaotong University Xi'an Shaanxi China
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Abstract

Altered extracellular biomechanical forces are increasingly implicated in a wide range of pathologies. However, the specific role of extracellular matrix stiffness in the pathogenesis of dental caries remains unclear. Although exosomes have been shown to regulate cell fate, it is still poorly understood how their biological journeys from biogenesis to secretion are affected by matrix stiffness. Here we find that matrix stiffness can enhance exosome release, thereby promoting odontogenic differentiation of dental pulp stem cells (DPSCs). Mechanistically, matrix stiffness promotes exosome release by modulating membrane curvature through Baiap2, which is tightly regulated by the stiffness‐activated PI3K‐AKT signaling pathway. Moreover, the release of exosomes was found to depend on mechanosensitive motor protein kinesin‐1 and its regulation of vesicular transport. Taken together, our results demonstrate that matrix stiffness plays an essential role in odontogenic differentiation of DPSCs by modulating exosome release. Our findings prove that the mechanosensitive process may play an underappreciated role in regulating exosome secretion and odontogenic differentiation in deep caries.

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Cite

BibTeXRIS
BibTeX
@article{jin2026matrix,
  title = {{Matrix Stiffness Enhances Odontogenic Differentiation of DPSCs through Membrane Curvature Protein Baiap2‐Modulated Exosome Release}},
  author = {Bilun Jin and Shaojie Dong and Yue Jia and Zhao Xu and Peiqi Liu and Zhaojing Ding and Yuxin Liao and Rui Zou and Jinsong Hu and Bo Cheng and Lin Niu},
  journal = {Advanced Science},
  year = {2026},
  month = aug,
  doi = {10.1002/advs.76908},
  url = {https://doi.org/10.1002/advs.76908},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Matrix Stiffness Enhances Odontogenic Differentiation of DPSCs through Membrane Curvature Protein Baiap2‐Modulated Exosome Release
AU  - Bilun Jin
AU  - Shaojie Dong
AU  - Yue Jia
AU  - Zhao Xu
AU  - Peiqi Liu
AU  - Zhaojing Ding
AU  - Yuxin Liao
AU  - Rui Zou
AU  - Jinsong Hu
AU  - Bo Cheng
AU  - Lin Niu
PY  - 2026
DA  - 2026/08/11
JO  - Advanced Science
DO  - 10.1002/advs.76908
UR  - https://doi.org/10.1002/advs.76908
AB  - Altered extracellular biomechanical forces are increasingly implicated in a wide range of pathologies. However, the specific role of extracellular matrix stiffness in the pathogenesis of dental caries remains unclear. Although exosomes have been shown to regulate cell fate, it is still poorly understood how their biological journeys from biogenesis to secretion are affected by matrix stiffness. Here we find that matrix stiffness can enhance exosome release, thereby promoting odontogenic differentiation of dental pulp stem cells (DPSCs). Mechanistically, matrix stiffness promotes exosome release by modulating membrane curvature through Baiap2, which is tightly regulated by the stiffness‐activated PI3K‐AKT signaling pathway. Moreover, the release of exosomes was found to depend on mechanosensitive motor protein kinesin‐1 and its regulation of vesicular transport. Taken together, our results demonstrate that matrix stiffness plays an essential role in odontogenic differentiation of DPSCs by modulating exosome release. Our findings prove that the mechanosensitive process may play an underappreciated role in regulating exosome secretion and odontogenic differentiation in deep caries.
SN  - 2198-3844
ER  -