Growth Profiles and Temporal Accumulation of Aniline-Blue-Reactive β-(1→3)-Glucan-Associated Material in Alcaligenes faecalis A12C Batch Cultures
6 affiliations
- 1University Institute of Animal Health and Food Safety (IUSA-ONE HEALTH) 2-Sanidad Animal de la Acuicultura y Especies Silvestres, Enfermedades Infecciosas y Seguridad Alimentaria, University of Las Palmas de Gran Canaria, 35416 Arucas, Spain
- 2Instituto de Investigación Sanitaria de Canarias (IISC), Eukaryotic-Prokaryotic Synergy-Comparative and Translational Medicine Group (Cardiorespiratory Infectious Diseases and Epidemioogy Area), Barranco de la Ballena s/n, 35019 Las Palmas de Gran Canaria, Spain
- 3Fundación Canaria del Instituto de Investigación Sanitaria de Canarias (FIISC), 35010 Las Palmas de Gran Canaria, Spain
- 4Banco Español de Algas, Instituto de Oceanografía y Cambio Global (IOCAG), Universidad de Las Palmas de Gran Canaria, 35214 Telde, Spain
- 5Research Unit, Hospital Universitario de Gran Canaria Dr. Negrín, Fundación del Instituto de Investigación Sanitaria de Canarias (FIISC), 35019 Las Palmas de Gran Canaria, Spain
- 6CIBER de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, 28029 Madrid, Spain
Abstract
Microbial exopolysaccharides (EPSs) are functional biopolymers of growing scientific and industrial interest because of their structural diversity, biocompatibility, and rheological properties. Microbial β-(1→3)-glucans are of particular biological and technological interest, and their production depends strongly on strain characteristics, medium composition, and culture conditions. This study characterized the temporal relationship among bacterial growth, viability, and aniline-blue reactivity in Alcaligenes faecalis A12C under batch growth and fermentation conditions. Growth turbidity, viable bacterial concentration, and β-(1→3)-glucan-associated signals were monitored over 96 h using spectrophotometric, microbiological, and microscopic approaches. In growth culture medium (GCM), OD600 increased after 24–30 h and reached approximately 2.4 at 72 h, coinciding with the highest viable count (2.44 × 1021 CFU/mL). Aniline-blue coloration became clearly detectable after 30 h and persisted through 96 h, when microscopy showed blue staining associated with bacterial cells. In fermentation culture medium (FCM), OD600 increased progressively to approximately 2.8 at 96 h, whereas viable counts followed a distinct temporal profile. Aniline-blue reactivity changed during the first 24 h and remained close to an A610 of approximately 1.0 thereafter. These findings indicate distinct temporal patterns among culture turbidity, viability, and aniline-blue reactivity and provide a framework for subsequent structural characterization of the detected material.
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BibTeX
@article{martelbenitez2026growth,
title = {{Growth Profiles and Temporal Accumulation of Aniline-Blue-Reactive β-(1→3)-Glucan-Associated Material in Alcaligenes faecalis A12C Batch Cultures}},
author = {C. J. Martel-Benítez and R. Henríquez-Cabrera and R. Alayón-Afonso and D. Padilla and F. Real and A. Martel-Quintana and J. L. Gómez-Pinchetti and C. Almeida and P. Santiago-Díaz and N. Robles-Antón and N. González-Caballero and J. L. Martín-Barrasa},
journal = {Polymers},
year = {2026},
month = nov,
doi = {10.3390/polym18212749},
url = {https://www.mdpi.com/2073-4360/18/21/2749},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Growth Profiles and Temporal Accumulation of Aniline-Blue-Reactive β-(1→3)-Glucan-Associated Material in Alcaligenes faecalis A12C Batch Cultures
AU - C. J. Martel-Benítez
AU - R. Henríquez-Cabrera
AU - R. Alayón-Afonso
AU - D. Padilla
AU - F. Real
AU - A. Martel-Quintana
AU - J. L. Gómez-Pinchetti
AU - C. Almeida
AU - P. Santiago-Díaz
AU - N. Robles-Antón
AU - N. González-Caballero
AU - J. L. Martín-Barrasa
PY - 2026
DA - 2026/11/05
JO - Polymers
DO - 10.3390/polym18212749
UR - https://www.mdpi.com/2073-4360/18/21/2749
AB - Microbial exopolysaccharides (EPSs) are functional biopolymers of growing scientific and industrial interest because of their structural diversity, biocompatibility, and rheological properties. Microbial β-(1→3)-glucans are of particular biological and technological interest, and their production depends strongly on strain characteristics, medium composition, and culture conditions. This study characterized the temporal relationship among bacterial growth, viability, and aniline-blue reactivity in Alcaligenes faecalis A12C under batch growth and fermentation conditions. Growth turbidity, viable bacterial concentration, and β-(1→3)-glucan-associated signals were monitored over 96 h using spectrophotometric, microbiological, and microscopic approaches. In growth culture medium (GCM), OD600 increased after 24–30 h and reached approximately 2.4 at 72 h, coinciding with the highest viable count (2.44 × 1021 CFU/mL). Aniline-blue coloration became clearly detectable after 30 h and persisted through 96 h, when microscopy showed blue staining associated with bacterial cells. In fermentation culture medium (FCM), OD600 increased progressively to approximately 2.8 at 96 h, whereas viable counts followed a distinct temporal profile. Aniline-blue reactivity changed during the first 24 h and remained close to an A610 of approximately 1.0 thereafter. These findings indicate distinct temporal patterns among culture turbidity, viability, and aniline-blue reactivity and provide a framework for subsequent structural characterization of the detected material.
SN - 2073-4360
ER -