Isolation of blood-derived extracellular vesicles for disease diagnosis: Methods, challenges, and applications
- 1Laboratory for the Analysis of Medicines (LAM), Department of Pharmacy, CIRM, University of Liege, Liège, 4000, Belgium
- 2Corresponding author.
Abstract
Extracellular vesicles (EVs) are a family of cell-derived membrane vesicles found in almost all body fluids. Blood-derived EVs are particularly attractive for clinical diagnostics due to their accessibility and systemic representation. However, the intrinsic complexity of EVs and the abundance of non-EV particles in blood have hindered the establishment of a standardized isolation method. Meanwhile, research on EV isolation is rapidly evolving, and there is a clear need for reviews that synthesize the recent advances in this field, particularly those focusing on blood-derived EVs. In this review, we provide a comprehensive overview of both established and emerging isolation techniques, summarizing their advantages, limitations, and applications reported over the past years, to provide methodological insights and guidance for future isolation studies.
Related
- Identification of candidate markers for the isolation of neuron-specific extracellular vesicles
- Electrochemical immunosensor-enabled liquid biopsy of extracellular vesicles for diagnosis of metabolic dysfunction-associated steatohepatitis
- Tissue‐Specific Isolation of Hepatocyte Extracellular Vesicles From Human Plasma Enables Tissue‐Resolved Proteomic and miRNA Profiling
- Controllable Cascade Aggregation Ion Programmed Nanomachines and Simple Isolation Enable Tumor‐Derived Small Extracellular Vesicles Detection for Enhanced Breast Cancer Assessment
- Human salivary extracellular vesicles: A comparative study of isolation techniques
BibTeX
@article{song2026isolation,
title = {{Isolation of blood-derived extracellular vesicles for disease diagnosis: Methods, challenges, and applications}},
author = {Xi Song and Jacques Crommen and Marianne Fillet},
journal = {Journal of Pharmaceutical Analysis},
year = {2026},
month = oct,
doi = {10.1016/j.jpha.2026.101640},
url = {https://doi.org/10.1016/j.jpha.2026.101640},
note = {CC BY},
}
RIS
TY - JOUR
TI - Isolation of blood-derived extracellular vesicles for disease diagnosis: Methods, challenges, and applications
AU - Xi Song
AU - Jacques Crommen
AU - Marianne Fillet
PY - 2026
DA - 2026/10/01
JO - Journal of Pharmaceutical Analysis
DO - 10.1016/j.jpha.2026.101640
UR - https://doi.org/10.1016/j.jpha.2026.101640
AB - Extracellular vesicles (EVs) are a family of cell-derived membrane vesicles found in almost all body fluids. Blood-derived EVs are particularly attractive for clinical diagnostics due to their accessibility and systemic representation. However, the intrinsic complexity of EVs and the abundance of non-EV particles in blood have hindered the establishment of a standardized isolation method. Meanwhile, research on EV isolation is rapidly evolving, and there is a clear need for reviews that synthesize the recent advances in this field, particularly those focusing on blood-derived EVs. In this review, we provide a comprehensive overview of both established and emerging isolation techniques, summarizing their advantages, limitations, and applications reported over the past years, to provide methodological insights and guidance for future isolation studies.
SN - 2095-1779
SN - 2214-0883
ER -