Programmable Nanozyme Gating: A Molecular Ejector Switch (MES) for High-Fidelity miRNA-21 Sensing
Abstract
Nucleotide-level variants in miRNA or pathogen genomes can critically reprogram gene-regulatory networks or enhance disease severity, highlighting the urgent need for sensing platforms capable of resolving subtle sequence discrepancies. In this article, we present a programmable molecular ejector switch (MES) that functions as a confinement-gating smart nanozyme for adaptive nucleic-acid sensing. By integrating the etb topology of mesoporous Co-IRMOF-74-II with single-stranded DNA (ssDNA) gatekeepers, the MES transforms passive MOF scaffolds into an active logical device. Driven by the balance between van der Waals attraction and electrostatic repulsion, the ssDNA gatekeepers can be reversibly accommodated within the pores to block mass transport through the MES. Upon target binding, hybridization displaces the ssDNA gatekeeper, while the resulting rigid duplex is less compatible with pore re-entry, thereby restoring the overall nanozyme activity. As a proof of concept, the miRNA-21 sensor furnishes accurate colorimetric readouts (LOD: 0.84 nM; log-linear range 1–300 nM), while concurrent fluorescent signals (LOD: 0.10 nM; linear range 0.2–50 nM) cross-validate the gating logic. The sensor outputs exhibit a monotonic dependence on the mismatch degree, enabling high-fidelity discrimination of multi-nucleotide variants. This modular confinement-gating strategy is readily adaptable to other nucleic acid biomarkers, offering a versatile scaffold for the development of intelligent diagnostic devices.
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BibTeX
@article{zhou2026programmable,
title = {{Programmable Nanozyme Gating: A Molecular Ejector Switch (MES) for High-Fidelity miRNA-21 Sensing}},
author = {Fei Zhou and Junjie He and Hangqi Wu and Li Tan and Yuji Zhang and Serge Cosnier and Junji Li and Dan Shan},
journal = {ACS Sensors},
year = {2026},
month = oct,
doi = {10.1021/acssensors.6c02388},
url = {https://hal.science/hal-05839346v1},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - Programmable Nanozyme Gating: A Molecular Ejector Switch (MES) for High-Fidelity miRNA-21 Sensing
AU - Fei Zhou
AU - Junjie He
AU - Hangqi Wu
AU - Li Tan
AU - Yuji Zhang
AU - Serge Cosnier
AU - Junji Li
AU - Dan Shan
PY - 2026
DA - 2026/10/17
JO - ACS Sensors
DO - 10.1021/acssensors.6c02388
UR - https://hal.science/hal-05839346v1
AB - Nucleotide-level variants in miRNA or pathogen genomes can critically reprogram gene-regulatory networks or enhance disease severity, highlighting the urgent need for sensing platforms capable of resolving subtle sequence discrepancies. In this article, we present a programmable molecular ejector switch (MES) that functions as a confinement-gating smart nanozyme for adaptive nucleic-acid sensing. By integrating the etb topology of mesoporous Co-IRMOF-74-II with single-stranded DNA (ssDNA) gatekeepers, the MES transforms passive MOF scaffolds into an active logical device. Driven by the balance between van der Waals attraction and electrostatic repulsion, the ssDNA gatekeepers can be reversibly accommodated within the pores to block mass transport through the MES. Upon target binding, hybridization displaces the ssDNA gatekeeper, while the resulting rigid duplex is less compatible with pore re-entry, thereby restoring the overall nanozyme activity. As a proof of concept, the miRNA-21 sensor furnishes accurate colorimetric readouts (LOD: 0.84 nM; log-linear range 1–300 nM), while concurrent fluorescent signals (LOD: 0.10 nM; linear range 0.2–50 nM) cross-validate the gating logic. The sensor outputs exhibit a monotonic dependence on the mismatch degree, enabling high-fidelity discrimination of multi-nucleotide variants. This modular confinement-gating strategy is readily adaptable to other nucleic acid biomarkers, offering a versatile scaffold for the development of intelligent diagnostic devices.
SN - 2379-3694
ER -