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Peer-reviewedCC BY 4.0International Journal of Molecular Sciences

Plasma DR-CALUX Bioactivity and Concurrent Clinical Biochemistry: A Secondary Cross-Sectional Analysis of SPES

  • Pellegrino Cerino1
  • Elvira De Rosa1,2
  • Maria Triassi1,2
  • Paolo Montuori1
  • Carlo Buonerba1
  1. 1Department of Public Health, University of Naples “Federico II”, Via Sergio Pansini 5, 80131 Naples, Italy
  2. 2Department of Human Sciences and Quality of Life Promotion, San Raffaele University, 00166 Rome, Italy
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Abstract

Plasma bioassays provide functional measures of environmental mixtures whose relationship with clinical biochemistry warrants investigation. We examined dioxin-responsive chemically activated luciferase gene expression (DR-CALUX) bioactivity in the 600-participant intensive-biomonitoring subgroup of SPES in Campania, Italy. Quantifiable bioactivity was available for 594 participants, of whom 592 entered the principal analyses. Mixed models assessed the lipid-standardised DR-CALUX index in relation to two primary thyroid outcomes, nine secondary outcomes, and an exploratory panel of 54 clinical markers. Per index doubling, thyroid-stimulating hormone differed by −7.23% (95% CI −14.96 to 1.21; Holm-adjusted p = 0.182), and free thyroxine differed by −0.0047 ng/dL (−0.0233 to 0.0139; adjusted p = 0.621). Alanine and aspartate aminotransferase activities were lower by 9.33% (−14.22 to −4.17) and 8.01% (−12.31 to −3.50), respectively; both met the hepatic-family Benjamini–Hochberg criterion. Inverse estimates persisted with separate lipid adjustment and attenuated but remained inverse after restriction using common laboratory-report upper thresholds for both aminotransferases. All 54 global Benjamini–Yekutieli-adjusted p values exceeded 0.05; only the aminotransferases met the Benjamini–Hochberg comparison. These secondary aminotransferase associations merit further investigation of their biological basis and clinical significance. The primary thyroid findings remain inconclusive.

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Cite

BibTeXRIS
BibTeX
@article{cerino2026plasma,
  title = {{Plasma DR-CALUX Bioactivity and Concurrent Clinical Biochemistry: A Secondary Cross-Sectional Analysis of SPES}},
  author = {Pellegrino Cerino and Elvira De Rosa and Maria Triassi and Paolo Montuori and Carlo Buonerba},
  journal = {International Journal of Molecular Sciences},
  year = {2026},
  month = nov,
  doi = {10.3390/ijms272110017},
  url = {https://www.mdpi.com/1422-0067/27/21/10017},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Plasma DR-CALUX Bioactivity and Concurrent Clinical Biochemistry: A Secondary Cross-Sectional Analysis of SPES
AU  - Pellegrino Cerino
AU  - Elvira De Rosa
AU  - Maria Triassi
AU  - Paolo Montuori
AU  - Carlo Buonerba
PY  - 2026
DA  - 2026/11/08
JO  - International Journal of Molecular Sciences
DO  - 10.3390/ijms272110017
UR  - https://www.mdpi.com/1422-0067/27/21/10017
AB  - Plasma bioassays provide functional measures of environmental mixtures whose relationship with clinical biochemistry warrants investigation. We examined dioxin-responsive chemically activated luciferase gene expression (DR-CALUX) bioactivity in the 600-participant intensive-biomonitoring subgroup of SPES in Campania, Italy. Quantifiable bioactivity was available for 594 participants, of whom 592 entered the principal analyses. Mixed models assessed the lipid-standardised DR-CALUX index in relation to two primary thyroid outcomes, nine secondary outcomes, and an exploratory panel of 54 clinical markers. Per index doubling, thyroid-stimulating hormone differed by −7.23% (95% CI −14.96 to 1.21; Holm-adjusted p = 0.182), and free thyroxine differed by −0.0047 ng/dL (−0.0233 to 0.0139; adjusted p = 0.621). Alanine and aspartate aminotransferase activities were lower by 9.33% (−14.22 to −4.17) and 8.01% (−12.31 to −3.50), respectively; both met the hepatic-family Benjamini–Hochberg criterion. Inverse estimates persisted with separate lipid adjustment and attenuated but remained inverse after restriction using common laboratory-report upper thresholds for both aminotransferases. All 54 global Benjamini–Yekutieli-adjusted p values exceeded 0.05; only the aminotransferases met the Benjamini–Hochberg comparison. These secondary aminotransferase associations merit further investigation of their biological basis and clinical significance. The primary thyroid findings remain inconclusive.
SN  - 1422-0067
ER  -