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Peer-reviewedCC BYBreeding science

QTL mapping for target leaf spot resistance in a Japanese-type cucumber.

  • Minh Thi Ho1
  • Ruikun Chen2
  • Sachiko Isobe3
  • Kenta Shirasawa3
  • Yosuke Yoshioka2
  1. 1Graduate School of Science and Technology, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.
  2. 2Institute of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.
  3. 3Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan.
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Abstract

Target leaf spot (TLS), caused by Corynespora cassiicola, is among the most economically important diseases of cucumber (Cucumis sativus L.). Because of the rising incidence of disease problems, much effort has been put toward resistance breeding. Here, we investigated the inheritance of high TLS resistance in a Japanese-type inbred line. QTL analyses were performed using F₂ and F2:3 families derived from a cross between the resistant and a susceptible (Beit Alpha-type) inbred line. We evaluated disease severity in F2:3 families in four independent assays and mapped a total of five TLS-resistance QTLs on chromosome (Chr.) 2, Chr. 5, and Chr. 6. The effect of the two major QTLs on Chr.5, Tls5.1 and Tls5.2, was confirmed through additional assays and verified in different genetic backgrounds. Fine mapping using recombinants in several generations narrowed down Tls5.2 to a 496.8-kb region with 69 genes, several of which are potential candidates involved in plant disease resistance. The newly identified resistance loci may confer more durable resistance when combined with previously characterized resistance genes or loci. Furthermore, molecular markers linked to TLS resistance derived from the Japanese-type inbred line could be effectively used to accelerate breeding for this trait.

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Cite

BibTeXRIS
BibTeX
@article{ho2026mapping,
  title = {{QTL mapping for target leaf spot resistance in a Japanese-type cucumber.}},
  author = {Minh Thi Ho and Ruikun Chen and Sachiko Isobe and Kenta Shirasawa and Yosuke Yoshioka},
  journal = {Breeding science},
  year = {2026},
  month = jun,
  doi = {10.1270/jsbbs.26005},
  url = {https://europepmc.org/article/MED/42936844},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - QTL mapping for target leaf spot resistance in a Japanese-type cucumber.
AU  - Minh Thi Ho
AU  - Ruikun Chen
AU  - Sachiko Isobe
AU  - Kenta Shirasawa
AU  - Yosuke Yoshioka
PY  - 2026
DA  - 2026/06/01
JO  - Breeding science
DO  - 10.1270/jsbbs.26005
UR  - https://europepmc.org/article/MED/42936844
AB  - Target leaf spot (TLS), caused by Corynespora cassiicola, is among the most economically important diseases of cucumber (Cucumis sativus L.). Because of the rising incidence of disease problems, much effort has been put toward resistance breeding. Here, we investigated the inheritance of high TLS resistance in a Japanese-type inbred line. QTL analyses were performed using F₂ and F2:3 families derived from a cross between the resistant and a susceptible (Beit Alpha-type) inbred line. We evaluated disease severity in F2:3 families in four independent assays and mapped a total of five TLS-resistance QTLs on chromosome (Chr.) 2, Chr. 5, and Chr. 6. The effect of the two major QTLs on Chr.5, Tls5.1 and Tls5.2, was confirmed through additional assays and verified in different genetic backgrounds. Fine mapping using recombinants in several generations narrowed down Tls5.2 to a 496.8-kb region with 69 genes, several of which are potential candidates involved in plant disease resistance. The newly identified resistance loci may confer more durable resistance when combined with previously characterized resistance genes or loci. Furthermore, molecular markers linked to TLS resistance derived from the Japanese-type inbred line could be effectively used to accelerate breeding for this trait.
SN  - 1344-7610
SN  - 1347-3735
ER  -