Mass spectrometry-guided isolation of naphthalenoid pulvinic acids from the Ectomycorrhizal Fungus Pisolithus arhizus
Abstract
Pisolithus arhizus is a worldwide distributed ectomycorrhizal fungus used in traditional medicine for wound healing and antimicrobial properties. It is also known for natural dye production, ecological significance, and ectomycorrhizal associations that detoxify polluted soils and support plant growth. In this study, an integrated dereplication strategy combining liquid chromatography-tandem mass spectrometry (LC-MS 2 ) with molecular networking was employed to investigate the phytochemical profile of the dichloromethane-methanol crude extract from the fruiting bodies of Pisolithus arhizus (Sclerodermataceae). The molecular networking analysis revealed distinct clusters of naphthalenoid pulvinic acid derivatives and enabled the rapid targeting, isolation, structure elucidation, and identification of a previously undescribed pisoquinone close analogue, named methylpisoquinone ( 1 ), alongside the known norbadione A. The isolated compounds were evaluated for their antimicrobial activities against Staphylococcus aureus , Escherichia coli , Candida albicans , and Mycobacterium bovis BCG . Both compounds were inactive at the tested concentrations.
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BibTeX
@article{tsamo2026mass,
title = {{Mass spectrometry-guided isolation of naphthalenoid pulvinic acids from the Ectomycorrhizal Fungus Pisolithus arhizus}},
author = {Armelle Tsamo and Pierre Le Pogam and André Njouonkou and Jérome Vanheuverzwijn and Panagiotis Tsampanis and Véronique Fontaine and Karine Leblanc and Somia Rharrabti and Pierre Mkounga and Augustin Nkengfack and Mehdi Beniddir},
journal = {Phytochemistry Letters},
year = {2026},
month = sep,
doi = {10.1016/j.phytol.2026.104123},
url = {https://hal.science/hal-05765489v1},
note = {CC BY},
}
RIS
TY - JOUR
TI - Mass spectrometry-guided isolation of naphthalenoid pulvinic acids from the Ectomycorrhizal Fungus Pisolithus arhizus
AU - Armelle Tsamo
AU - Pierre Le Pogam
AU - André Njouonkou
AU - Jérome Vanheuverzwijn
AU - Panagiotis Tsampanis
AU - Véronique Fontaine
AU - Karine Leblanc
AU - Somia Rharrabti
AU - Pierre Mkounga
AU - Augustin Nkengfack
AU - Mehdi Beniddir
PY - 2026
DA - 2026/09/26
JO - Phytochemistry Letters
DO - 10.1016/j.phytol.2026.104123
UR - https://hal.science/hal-05765489v1
AB - Pisolithus arhizus is a worldwide distributed ectomycorrhizal fungus used in traditional medicine for wound healing and antimicrobial properties. It is also known for natural dye production, ecological significance, and ectomycorrhizal associations that detoxify polluted soils and support plant growth. In this study, an integrated dereplication strategy combining liquid chromatography-tandem mass spectrometry (LC-MS 2 ) with molecular networking was employed to investigate the phytochemical profile of the dichloromethane-methanol crude extract from the fruiting bodies of Pisolithus arhizus (Sclerodermataceae). The molecular networking analysis revealed distinct clusters of naphthalenoid pulvinic acid derivatives and enabled the rapid targeting, isolation, structure elucidation, and identification of a previously undescribed pisoquinone close analogue, named methylpisoquinone ( 1 ), alongside the known norbadione A. The isolated compounds were evaluated for their antimicrobial activities against Staphylococcus aureus , Escherichia coli , Candida albicans , and Mycobacterium bovis BCG . Both compounds were inactive at the tested concentrations.
SN - 1874-3900
ER -