Skip to content
DraconiticResearch
Search Sources API
203,952 studies31 sourcesUpdated 8 min ago
Peer-reviewedCC BY 4.0Nutrients

Human Intestinal Microbiota-Derived Short-Chain Fatty Acid and Lactate Profiles In Vivo and In Vitro: A Structured Review and Comparative Case Study

  • Agnieszka Rudzka1
  • Ondřej Patloka2
  • Marcin Kruk3
  • Magdalena Płecha4,5
  • Marek Zborowski6
  • Tomasz Królikowski7
  • Michał Oczkowski7
  • Danuta Kołożyn-Krajewska1,3
  • Marcelina Karbowiak3
  • Wioletta Mosiej3
  • Renata Barczyńska-Felusiak1
  • Dorota Zielińska3
7 affiliations
  1. 1Department of Dietetics and Food Studies, Faculty of Science and Technology, Jan Dlugosz University in Czestochowa, Al. Armii Krajowej 13/15, 42-200 Częstochowa, Poland
  2. 2Department of Food Technology, Faculty of AgriSciences, Mendel University in Brno, Zemědělská 1, 61300 Brno, Czech Republic
  3. 3Department of Gastronomy and Food Health Quality, Faculty of Health Sciences, Warsaw University of Life Sciences (WULS-SGGW), Nowoursynowska 159 C, 02-776 Warsaw, Poland
  4. 4Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland
  5. 5Institute of Evolutionary Biology, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089 Warsaw, Poland
  6. 6The Faculty of Medicine and Health Sciences, University of Applied Sciences in Nowy Sącz, Kościuszki 2G, 33-300 Nowy Sącz, Poland
  7. 7Department of Dietetics, Faculty of Health Sciences, Warsaw University of Life Sciences (WULS-SGGW) Nowoursynowska 159 C, 02-776 Warsaw, Poland
Open at sourceDOICite

Abstract

Background: The transfer of human intestinal microbiota from in vivo to in vitro conditions alters its structure. This transition may trigger functional changes in the microbial community, such as its ability to produce short-chain fatty acids (SCFAs) and lactate. Methods: This study evaluated SCFAs and lactate profiles in the dynamic simulator and in the feces of a human volunteer who donated a sample for the inoculation of the system. Changes in SCFA and lactate levels were monitored over a 28-day dietary intervention and a parallel 14-day probiotic supplementation. Results: Microbial and dietary variation were not significant predictors of variability in SCFA and lactate profiles. In contrast, culturing environment and probiotic intervention exerted significant effects on SCFAs and lactate. The probiotic intervention appeared to influence SCFA and lactate profiles under in vitro conditions, whereas no similar effect was observed in vivo. Furthermore, the abundance of individual microbial genera was mostly weakly correlated with SCFA and lactate levels, and the direction of these correlations was inconsistent across two microbiota culturing environments. Conclusion: Our findings suggest that structural changes in the microbiota during its transfer from in vivo to in vitro conditions are accompanied by alterations in microbial functions, such as SCFA and lactate levels or response to interventions. However, these responses align with data from other human studies, supporting the ability of dynamic in vitro models to simulate key functions of the intestinal microbiota. Further optimization is required to accurately model personalized microbiota responses.

Related

  1. Gut microbiota-derived tryptophan metabolites and inflammatory factors enhance prediction of early neurological deterioration after intravenous thrombolysis in stroke patients: a comparative model study
    Wei Li, Zhiyan Feng, Chunrong Zeng +4
    Frontiers in NeurologyPeer-reviewedCC BY 4.0Full textEpidemiology & public healthCrossrefEurope PMCPMC
  2. Heat-Inactivated Lacticaseibacillus paracasei PC-01 Alleviates DSS-Induced Colitis in Mice by Modulating Gut Microbiota and Intestinal Barrier Function
    Xinxue Zhao, Chang Yang, Lu Gao +6
    NutrientsPeer-reviewedCC BYFull textNutritionEurope PMC
  3. Contributions of the gut microbiota and intestinal gluconeogenesis to metabolic adaptations in a mouse model of anorexia nervosa.
    S.-P. Hviid-Chen, Z. Boudra, N. Kassis +13
    bioRxivPreprintAll rights reservedFull textPhysiologybioRxiv
  4. Aspergillus species and gut microbiota-mediated biotransformation of epigallocatechin gallate: From dark tea fermentation to intestinal metabolism.
    Zexin An, Rongrong Ma, Xiangxiang Huang +5
    Current research in food sciencePeer-reviewedCC BYFull textGeneral medicineEurope PMCPMC
  5. Intestinal Responses to Short-Term Hexaflumuron Exposure in Grass Carp (Ctenopharyngodon idella): A Combined Physiological, Transcriptomic, and Gut Microbiota Perspective.
    Shun Zhou, Jiangtao Li, Qiuhong Yang +1
    Animals : an open access journal from MDPIPeer-reviewedCC BYFull textPharmacology & toxicologyEurope PMC

Cite

BibTeXRIS
BibTeX
@article{rudzka2026human,
  title = {{Human Intestinal Microbiota-Derived Short-Chain Fatty Acid and Lactate Profiles In Vivo and In Vitro: A Structured Review and Comparative Case Study}},
  author = {Agnieszka Rudzka and Ondřej Patloka and Marcin Kruk and Magdalena Płecha and Marek Zborowski and Tomasz Królikowski and Michał Oczkowski and Danuta Kołożyn-Krajewska and Marcelina Karbowiak and Wioletta Mosiej and Renata Barczyńska-Felusiak and Dorota Zielińska},
  journal = {Nutrients},
  year = {2026},
  month = nov,
  doi = {10.3390/nu18213701},
  url = {https://www.mdpi.com/2072-6643/18/21/3701},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Human Intestinal Microbiota-Derived Short-Chain Fatty Acid and Lactate Profiles In Vivo and In Vitro: A Structured Review and Comparative Case Study
AU  - Agnieszka Rudzka
AU  - Ondřej Patloka
AU  - Marcin Kruk
AU  - Magdalena Płecha
AU  - Marek Zborowski
AU  - Tomasz Królikowski
AU  - Michał Oczkowski
AU  - Danuta Kołożyn-Krajewska
AU  - Marcelina Karbowiak
AU  - Wioletta Mosiej
AU  - Renata Barczyńska-Felusiak
AU  - Dorota Zielińska
PY  - 2026
DA  - 2026/11/07
JO  - Nutrients
DO  - 10.3390/nu18213701
UR  - https://www.mdpi.com/2072-6643/18/21/3701
AB  - Background: The transfer of human intestinal microbiota from in vivo to in vitro conditions alters its structure. This transition may trigger functional changes in the microbial community, such as its ability to produce short-chain fatty acids (SCFAs) and lactate. Methods: This study evaluated SCFAs and lactate profiles in the dynamic simulator and in the feces of a human volunteer who donated a sample for the inoculation of the system. Changes in SCFA and lactate levels were monitored over a 28-day dietary intervention and a parallel 14-day probiotic supplementation. Results: Microbial and dietary variation were not significant predictors of variability in SCFA and lactate profiles. In contrast, culturing environment and probiotic intervention exerted significant effects on SCFAs and lactate. The probiotic intervention appeared to influence SCFA and lactate profiles under in vitro conditions, whereas no similar effect was observed in vivo. Furthermore, the abundance of individual microbial genera was mostly weakly correlated with SCFA and lactate levels, and the direction of these correlations was inconsistent across two microbiota culturing environments. Conclusion: Our findings suggest that structural changes in the microbiota during its transfer from in vivo to in vitro conditions are accompanied by alterations in microbial functions, such as SCFA and lactate levels or response to interventions. However, these responses align with data from other human studies, supporting the ability of dynamic in vitro models to simulate key functions of the intestinal microbiota. Further optimization is required to accurately model personalized microbiota responses.
SN  - 2072-6643
ER  -