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Peer-reviewedCC BYComputational and structural biotechnology journal

Host-Associated Binding Patterns in Predicted Structural Models of Circovirus Capsid-Importin Complexes.

  • Yue Wang1
  • Junping Wen1
  • Jipei Zhang1
  • Shujun Yang2
  • Cuicui Meng2
  • Bingyu Yu1
  • Yitong Ye1
  • Yinsheng Lin1
  • Wenchang Xue1
  • Jinling Huang1
  • Jidang Chen1,3
  • Tong Wang2
  1. 1School of Animal Science and Technology, Foshan University, Foshan 528225, Guangdong, China.
  2. 2Department of Emergency, The Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen 518033, Guangdong, China.
  3. 3Agricultural Teaching and Research Base, Foshan University, Foshan 528225, Guangdong, China.
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Abstract

The nuclear entry of the capsid protein is a critical step in the life cycle of circoviruses, but whether capsid proteins from different circoviruses differ in their importin interactions remained unclear. Here, we modeled 298 unique circovirus capsid protein-importin pairs using AlphaFold 3. After conformational clustering and ZDOCK concordance filtering, 603 representative interfaces were retained for analysis. We extracted ΔiG values, interface features, and nuclear localization signal (NLS)-specific interface properties by PDBePISA and analyzed them with statistical tests. Capsid protein (Cap)-importin-9 (IPO9) complexes showed the lowest mean ΔiG among all tested circoviruses, identifying IPO9 as a candidate broadly interacting importin among the viruses examined. Across all host classes, Cap-importin β complexes showed a lower mean ΔiG than Cap-importin α or Cap-transportin complexes. Predicted ΔiG patterns differed across host classes, with distinct profiles for avian, mammalian, and fish circoviruses. Predicted ΔiG patterns and NLS interface features, including basic residue ratio, interface occupancy, and net charge, differed across avian, mammalian, and fish circoviruses. Together, these results provide a set of testable predictions regarding circovirus Cap-importin interfaces and highlight the predicted NLS interface as a region for further investigation.

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Cite

BibTeXRIS
BibTeX
@article{wang2026hostassociated,
  title = {{Host-Associated Binding Patterns in Predicted Structural Models of Circovirus Capsid-Importin Complexes.}},
  author = {Yue Wang and Junping Wen and Jipei Zhang and Shujun Yang and Cuicui Meng and Bingyu Yu and Yitong Ye and Yinsheng Lin and Wenchang Xue and Jinling Huang and Jidang Chen and Tong Wang},
  journal = {Computational and structural biotechnology journal},
  year = {2026},
  month = nov,
  doi = {10.34133/csbj.0253},
  url = {https://europepmc.org/article/MED/42996474},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Host-Associated Binding Patterns in Predicted Structural Models of Circovirus Capsid-Importin Complexes.
AU  - Yue Wang
AU  - Junping Wen
AU  - Jipei Zhang
AU  - Shujun Yang
AU  - Cuicui Meng
AU  - Bingyu Yu
AU  - Yitong Ye
AU  - Yinsheng Lin
AU  - Wenchang Xue
AU  - Jinling Huang
AU  - Jidang Chen
AU  - Tong Wang
PY  - 2026
DA  - 2026/11/06
JO  - Computational and structural biotechnology journal
DO  - 10.34133/csbj.0253
UR  - https://europepmc.org/article/MED/42996474
AB  - The nuclear entry of the capsid protein is a critical step in the life cycle of circoviruses, but whether capsid proteins from different circoviruses differ in their importin interactions remained unclear. Here, we modeled 298 unique circovirus capsid protein-importin pairs using AlphaFold 3. After conformational clustering and ZDOCK concordance filtering, 603 representative interfaces were retained for analysis. We extracted ΔiG values, interface features, and nuclear localization signal (NLS)-specific interface properties by PDBePISA and analyzed them with statistical tests. Capsid protein (Cap)-importin-9 (IPO9) complexes showed the lowest mean ΔiG among all tested circoviruses, identifying IPO9 as a candidate broadly interacting importin among the viruses examined. Across all host classes, Cap-importin β complexes showed a lower mean ΔiG than Cap-importin α or Cap-transportin complexes. Predicted ΔiG patterns differed across host classes, with distinct profiles for avian, mammalian, and fish circoviruses. Predicted ΔiG patterns and NLS interface features, including basic residue ratio, interface occupancy, and net charge, differed across avian, mammalian, and fish circoviruses. Together, these results provide a set of testable predictions regarding circovirus Cap-importin interfaces and highlight the predicted NLS interface as a region for further investigation.
SN  - 2001-0370
ER  -