Novel recombinant bovine papular stomatitis virus expressing foot-and-mouth disease virus-like particles elicits neutralizing antibodies in cattle.
4 affiliations
- 1Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Urbana, IL, USA
- 2School of Veterinary Medicine and Biomedical Sciences and Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, USA
- 3Instituto de Virología e Innovaciones Tecnológicas, CICVyA, INTA-CONICET, Buenos Aires, Argentina
- 4WOAH FMD Reference Laboratory, SENASA, Buenos Aires, Argentina
Abstract
Foot-and-mouth disease (FMD) remains a major burden in endemic regions, where inactivated vaccines are constrained by cost, short duration of immunity, cold-chain dependence and high-containment manufacturing. Here, we engineered a recombinant bovine papular stomatitis virus (rBPSV) expressing the FMD virus (FMDV) A24 Cruzeiro capsid precursor P1-2A together with an attenuated 3C protease (3CproL127P). Infection of ovine foetal turbinate cells resulted in FMDV capsid protein expression and assembly of 25-30 nm icosahedral virus-like particles (VLPs). Intramuscular immunization of two BPSV-seropositive calves with rBPSV (10⁷ TCID₅₀ on days 0, 21 and 35) induced anti-FMDV humoral responses. By day 28, liquid-phase blocking ELISA titres exceeded laboratory-defined protective threshold (≥1.9 log₁₀), and homologous neutralizing titres against A24 Cruzeiro surpassed the threshold associated with protection (≥1.36 log₁₀). Intra-serotypic cross-neutralization was observed against A/Argentina/2001, whereas no neutralization was detected against heterologous serotype O₁ Campos. Pre-existing anti-BPSV antibodies did not prevent induction of neutralizing responses. These findings establish the first proof of concept that BPSV can serve as a cattle-adapted vector platform for delivery of FMDV VLPs.
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BibTeX
@article{chambaro2026novel,
title = {{Novel recombinant bovine papular stomatitis virus expressing foot-and-mouth disease virus-like particles elicits neutralizing antibodies in cattle.}},
author = {Herman M. Chambaro and Gustavo Delhon and María Cruz Miraglia and Sabrina Galdo-Novo and Ana Taffarel and Byung-Joon Seung and Sushil Khatiwada and Daniel Mariano Pérez-Filgueira and Daniel L. Rock},
journal = {The Journal of general virology},
year = {2026},
month = nov,
doi = {10.1099/jgv.0.002344},
url = {https://europepmc.org/article/MED/42970347},
note = {CC BY},
}
RIS
TY - JOUR
TI - Novel recombinant bovine papular stomatitis virus expressing foot-and-mouth disease virus-like particles elicits neutralizing antibodies in cattle.
AU - Herman M. Chambaro
AU - Gustavo Delhon
AU - María Cruz Miraglia
AU - Sabrina Galdo-Novo
AU - Ana Taffarel
AU - Byung-Joon Seung
AU - Sushil Khatiwada
AU - Daniel Mariano Pérez-Filgueira
AU - Daniel L. Rock
PY - 2026
DA - 2026/11/01
JO - The Journal of general virology
DO - 10.1099/jgv.0.002344
UR - https://europepmc.org/article/MED/42970347
AB - Foot-and-mouth disease (FMD) remains a major burden in endemic regions, where inactivated vaccines are constrained by cost, short duration of immunity, cold-chain dependence and high-containment manufacturing. Here, we engineered a recombinant bovine papular stomatitis virus (rBPSV) expressing the FMD virus (FMDV) A24 Cruzeiro capsid precursor P1-2A together with an attenuated 3C protease (3CproL127P). Infection of ovine foetal turbinate cells resulted in FMDV capsid protein expression and assembly of 25-30 nm icosahedral virus-like particles (VLPs). Intramuscular immunization of two BPSV-seropositive calves with rBPSV (10⁷ TCID₅₀ on days 0, 21 and 35) induced anti-FMDV humoral responses. By day 28, liquid-phase blocking ELISA titres exceeded laboratory-defined protective threshold (≥1.9 log₁₀), and homologous neutralizing titres against A24 Cruzeiro surpassed the threshold associated with protection (≥1.36 log₁₀). Intra-serotypic cross-neutralization was observed against A/Argentina/2001, whereas no neutralization was detected against heterologous serotype O₁ Campos. Pre-existing anti-BPSV antibodies did not prevent induction of neutralizing responses. These findings establish the first proof of concept that BPSV can serve as a cattle-adapted vector platform for delivery of FMDV VLPs.
SN - 0022-1317
SN - 1465-2099
ER -